Abstract
Monocyte tumor cell line PU5-1.8 does not normally produce colony- stimulating activity (CSA) required by granulocyte and macrophage progenitors to proliferate and mature in agar. However, CSA is induced in the culture line by as little as 10 ng/ml endotoxic lipopolysaccharide (LPS), with maximum CSA production and release to the medium between 2 and 3 days of incubation. Derived lipid A, but not alkali-treated LPS, is also active. Induction requires RNA and protein synthesis, but is not blocked by mitomycin C or Colcemid. Other inducers of CSA include Mycobacterium Bacillus Calmette-Guerin, tuberculin protein preparation purified protein derivative, zymosan, and phorbol myristate. All inducing agents are specific inhibitors of the monocyte tumor cell proliferation in vitro. Latex beads, another macrophage-activating agent, are rapidly phagocytosed by PU5-1.8 cells, but neither inhibit growth nor induce CSA.
Full Text
The Full Text of this article is available as a PDF (457.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Apte R. N., Galanos C., Pluznik D. H. Lipid A, the active part of bacterial endotoxins in inducing serum colony stimulating activity and proliferation of splenic granulocyte/macrophage progenitor cells. J Cell Physiol. 1976 Jan;87(1):71–78. doi: 10.1002/jcp.1040870110. [DOI] [PubMed] [Google Scholar]
- Chervenick P. A., LoBuglio A. F. Human blood monocytes: stimulators of granulocyte and mononuclear colony formation in vitro. Science. 1972 Oct 13;178(4057):164–166. doi: 10.1126/science.178.4057.164. [DOI] [PubMed] [Google Scholar]
- Cline M. J., Rothman B., Golde D. W. Effect of endotoxin on the production of colony-stimulating factor by human monocytes and macrophages. J Cell Physiol. 1974 Oct;84(2):193–196. doi: 10.1002/jcp.1040840205. [DOI] [PubMed] [Google Scholar]
- Eaves A. C., Bruce W. R. In vitro production of colony-stimulating activity. I. Exposure of mouse peritoneal cells to endotoxin. Cell Tissue Kinet. 1974 Jan;7(1):19–30. doi: 10.1111/j.1365-2184.1974.tb00395.x. [DOI] [PubMed] [Google Scholar]
- Friend C., Scher W. Stimulation by dimethyl sulfoxide of erythroid differentiation and hemoglobin synthesis in murine virus-induced leukemic cells. Ann N Y Acad Sci. 1975 Jan 27;243:155–163. doi: 10.1111/j.1749-6632.1975.tb25354.x. [DOI] [PubMed] [Google Scholar]
- Gronowicz E., Biberfeld P., Wahren B., Coutinho A. Characterization of dextran-sulfate-sensitive cells. Scand J Immunol. 1976;5(5):573–582. doi: 10.1111/j.1365-3083.1976.tb00312.x. [DOI] [PubMed] [Google Scholar]
- Moore M. A., Williams N., Metcalf D. In vitro colony formation by normal and leukemic human hematopoietic cells: interaction between colony-forming and colony-stimulating cells. J Natl Cancer Inst. 1973 Mar;50(3):591–602. doi: 10.1093/jnci/50.3.591. [DOI] [PubMed] [Google Scholar]
- Ralph P., Nakoinz I. Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 1977 Feb;37(2):546–550. [PubMed] [Google Scholar]
- Ralph P., Nakoinz I., Raschke W. C. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1268–1275. doi: 10.1016/s0006-291x(74)80421-3. [DOI] [PubMed] [Google Scholar]
- Reed W. P., Lucas Z. J. Cytotoxic activity of lymphocytes. V. Role of soluble toxin in macrophage-inhibited cultures of tumor cells. J Immunol. 1975 Aug;115(2):395–404. [PubMed] [Google Scholar]
- Ruscetti F. W., Chervenick P. A. Release of colony-stimulating factor from monocytes by endotoxin and polyinosinic-polycytidylic acid. J Lab Clin Med. 1974 Jan;83(1):64–72. [PubMed] [Google Scholar]