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. 1977 Aug 1;146(2):422–434. doi: 10.1084/jem.146.2.422

Expression of AKR murine leukemia virus gp71-like and BALB(X) gp-71- like antigens in normal mouse tissues in the absence of overt virus expression

PMCID: PMC2180780  PMID: 195002

Abstract

By competition radioimmune assays with antisera against AKR murine leukemia virus (MuLV) gp 71 or antisera against xenotropic virus, and iodinated AKR MuLV gp71 or BALB(X) gp71, antigens serologically indistinguishable from the viral antigens can be detected in tissues of normal mice in the absence of overt virus expression. An antigen serologically indistinguishable from AKR MuLV gp71 can be readily detected in normal bone marrow cells of the common strains of mice including NIH Swiss, 129/J, and SWR/J, as well as in Mus cervicolor and Mus musculus casteneus. In contrast, this antigen is not detected in normal spleen, thymus, lymph nodes, or serum. Similarly, an antigen serologically indistinguishable from BALB(X) gp71 was found in all normal mouse sera examined. This antigen was not present in fetal liver, perfused adult liver, thymus, spleen, lymph nodes, or bone marrow of the mice examined. An equivalent antigen was detected in sera from Mus musculus casteneus but not in sera from Mus cervicolor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaronson S. A., Stephenson J. R. Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2055–2058. doi: 10.1073/pnas.70.7.2055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Callahan R., Benveniste R. E., Lieber M. M., Todaro G. J. Nucleic acid homology of murine type-C viral genes. J Virol. 1974 Dec;14(6):1394–1403. doi: 10.1128/jvi.14.6.1394-1403.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. Evidence that the AKR murine-leukemia-virus genome is complete in DNA of the high-virus AKR mouse and incomplete in the DNA of the "virus-negative" NIH mouse. Proc Natl Acad Sci U S A. 1974 Jan;71(1):167–171. doi: 10.1073/pnas.71.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Del Vellano B. C., Nave B., Croker B. P., Lerner R. A., Dixon F. J. The oncornavirus glycoprotein gp69/71: a constituent of the surface of normal and malignant thymocytes. J Exp Med. 1975 Jan 1;141(1):172–187. doi: 10.1084/jem.141.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Del Villano B. C., Lerner R. A. Relationship between the oncornavirus gene product gp70 and a major protein secretion of the mouse genital tract. Nature. 1976 Feb 12;259(5543):497–499. doi: 10.1038/259497a0. [DOI] [PubMed] [Google Scholar]
  6. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hino S., Stephenson J. R., Aaronson S. A. Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. J Virol. 1976 Jun;18(3):933–941. doi: 10.1128/jvi.18.3.933-941.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huebner R. J., Kelloff G. J., Sarma P. S., Lane W. T., Turner H. C., Gilden R. V., Oroszlan S., Meier H., Myers D. D., Peters R. L. Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis. Proc Natl Acad Sci U S A. 1970 Sep;67(1):366–376. doi: 10.1073/pnas.67.1.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Huebner R. J., Todaro G. J. Oncogenes of RNA tumor viruses as determinants of cancer. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1087–1094. doi: 10.1073/pnas.64.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ihle J. N., Denny T. P., Bolognesi D. P. Purification and serological characterization of the major envelope glycoprotein from AKR murine leukemia virus and its reactivity with autogenous immune sera from mice. J Virol. 1976 Mar;17(3):727–736. doi: 10.1128/jvi.17.3.727-736.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ihle J. N., Domotor J. J., Jr, Bengali K. M. Strain-dependent development of an autogenous immune response in mice to endogenous C type viruses. Bibl Haematol. 1975 Oct;(43):177–179. doi: 10.1159/000399122. [DOI] [PubMed] [Google Scholar]
  12. Ihle J. N., Hanna M. G., Jr, Roberson L. E., Kenney F. T. Autogenous immunity to endogenous RNA tumor virus. Identification of antibody reactivity to select viral antigens. J Exp Med. 1974 Jun 1;139(6):1568–1581. doi: 10.1084/jem.139.6.1568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ihle J. N., Joseph D. R., Pazmino N. H. Radiation leukemia in C57BL/6 mice. II. Lack of ecotropic virus expression in the majority of lymphomas. J Exp Med. 1976 Dec 1;144(6):1406–1423. doi: 10.1084/jem.144.6.1406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ihle J. N., McEwan R., Bengali K. Radiation leukemia in C57BL/6 mice. I. Lack of serological evidence for the role of endogenous ecotropic viruses in pathogenesis. J Exp Med. 1976 Dec 1;144(6):1391–1405. doi: 10.1084/jem.144.6.1391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kennel S. J. Purification of a glycoprotein from mouse ascites fluid by immunoaffinity chromatography which is related to the major glycoprotein of murine leukemia viruses. Immunologic and structural comparison with purified viral glycoproteins. J Biol Chem. 1976 Oct 25;251(20):6197–6204. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lee J. C., Ihle J. N. Inhibition of normal mouse lymphocyte mitogen responses by xenogeneic or allogeneic antibodies to the MuLV glycoprotein gp71. J Immunol. 1976 Sep;117(3):1033–1038. [PubMed] [Google Scholar]
  18. Lerner R. A., Wilson C. B., Villano B. C., McConahey P. J., Dixon F. J. Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycorprotein, gp70. J Exp Med. 1976 Jan 1;143(1):151–166. doi: 10.1084/jem.143.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parks W. P., Livingston D. M., Todaro G. J., Benveniste R. E., Scolnick E. M. Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues. J Exp Med. 1973 Mar 1;137(3):622–635. doi: 10.1084/jem.137.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Strand M., Lilly F., August J. T. Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3682–3686. doi: 10.1073/pnas.71.9.3682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tung J. S., Vitetta E. S., Fleissner E., Boyse E. A. Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus. J Exp Med. 1975 Jan 1;141(1):198–205. doi: 10.1084/jem.141.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]

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