Abstract
Mouse alveolar macrophages (AM) cultured in the absence of serum secrete an elastolytic enzyme. The elastase from AM resembles the previously described elastase from peritoneal macrophages (PM) in pH optimum and inhibition profile. The macrophage enzymes do not appear to be stored, and with periodic changes in the culture medium, accumulate extracellularly for up to 10 days. Resident PM produce barely detectable levels of extracellular elastase unless given a phagocytic load. Thioglycollate-stimulated peritoneal exudative macrophages (PEM), however, secret easily detectable levels of elastase, which can be further increased with a phagocytic load. Without any additional stimulation, AM secret an elastolytic activity comparable to that of the PEM receiving a phagocytic load, but unlike PM they do not increase elastase secretion after phagocytosis.
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Selected References
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- Brain J. D., Frank R. Alveolar macrophage adhesion: wash electrolyte composition and free cell yield. J Appl Physiol. 1973 Jan;34(1):75–80. doi: 10.1152/jappl.1973.34.1.75. [DOI] [PubMed] [Google Scholar]
- Gordon S., Todd J., Cohn Z. A. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. J Exp Med. 1974 May 1;139(5):1228–1248. doi: 10.1084/jem.139.5.1228. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris J. O., Olsen G. N., Castle J. R., Maloney A. S. Comparison of proteolytic enzyme activity in pulmonary alveolar macrophages and blood leukocytes in smokers and nonsmokers. Am Rev Respir Dis. 1975 May;111(5):579–586. doi: 10.1164/arrd.1975.111.5.579. [DOI] [PubMed] [Google Scholar]
- Harris J. O., Swenson E. W., Johnson J. E., 3rd Human alveolar macrophages: comparison of phagocytic ability, glucose utilization, and ultrastructure in smokers and nonsmokers. J Clin Invest. 1970 Nov;49(11):2086–2096. doi: 10.1172/JCI106426. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayes J. A., Korthy A., Snider G. L. The pathology of elastase-induced panacinar emphysema in hamsters. J Pathol. 1975 Sep;117(1):1–14. doi: 10.1002/path.1711170102. [DOI] [PubMed] [Google Scholar]
- Kaplan P. D., Kuhn C., Pierce J. A. The induction of emphysema with elastase. I. The evolution of the lesion and the influence of serum. J Lab Clin Med. 1973 Sep;82(3):349–356. [PubMed] [Google Scholar]
- Niewoehner D. E., Kleinerman J., Rice D. B. Pathologic changes in the peripheral airways of young cigarette smokers. N Engl J Med. 1974 Oct 10;291(15):755–758. doi: 10.1056/NEJM197410102911503. [DOI] [PubMed] [Google Scholar]
- Powers J. C., Tuhy P. M. Active-site specific inhibitors of elastase. Biochemistry. 1973 Nov 6;12(23):4767–4774. doi: 10.1021/bi00747a032. [DOI] [PubMed] [Google Scholar]
- Stewart C. C., Lin H. S., Adles C. Proliferation and colony-forming ability of peritoneal exudate cells in liquid culture. J Exp Med. 1975 May 1;141(5):1114–1132. doi: 10.1084/jem.141.5.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unkeless J. C., Gordon S., Reich E. Secretion of plasminogen activator by stimulated macrophages. J Exp Med. 1974 Apr 1;139(4):834–850. doi: 10.1084/jem.139.4.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weinbaum G., Takamoto M., Sloan B., Kimbel P. Lung antiproteinase: a potential defense against emphysema development. Am Rev Respir Dis. 1976 Feb;113(2):245–248. doi: 10.1164/arrd.1976.113.2.245. [DOI] [PubMed] [Google Scholar]
- Werb Z., Gordon S. Elastase secretion by stimulated macrophages. Characterization and regulation. J Exp Med. 1975 Aug 1;142(2):361–377. doi: 10.1084/jem.142.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Werb Z., Gordon S. Secretion of a specific collagenase by stimulated macrophages. J Exp Med. 1975 Aug 1;142(2):346–360. doi: 10.1084/jem.142.2.346. [DOI] [PMC free article] [PubMed] [Google Scholar]