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. 1977 Oct 1;146(4):1019–1032. doi: 10.1084/jem.146.4.1019

In vitro analysis of allogeneic lymphocyte interaction. I. Characterization and cellular origin of an Ia-positive helper factor- allogeneic effect factor

TL Delovitch, HO McDevitt
PMCID: PMC2180825  PMID: 302311

Abstract

A soluble allogeneic effect factor (AEF) was produced by using H-2 congenic mouse strains and a serum.free cell culture medium. An AEF derived from untreated activated responder cells and irradiated stimulator cells provided helper cell function in a primary and secondary antibody response for both T-cell-depleted responder B cells and stimulator B cells. This interaction may be determined by genes situated in the I-A and I-B regions: additional K-region control was not excluded. Ia antigens, but neither H-2 nor Ig determinants are molecular constituents of AEF. The active components of this AEF consist, in part, of Ia antigens derived from both the activated responder cell population and irradiated stimulator cell population. An AEF derived from Ia negative responder cells and irradiated T-cell- depleted stimulator cells helps a secondary antibody response of T-cell- depleted stimulator B cells but not responder B cells. This genetically restricted AEF contains Ia antigens determined by the stimulator haplotype but not the responder haplotype. The priming antigen, DNP- keyhole limpet hemocyanin, is not a component of restricted AEF. The data suggest that restricted AEF may be a product of a stimulator B cell and/or macrophage. They support the hypothesis that the recognition by allogeneic T cells of Ia antigens on B cells activates the B cell to IgG antibody production.

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Selected References

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  1. Armerding D., Katz D. H. Activation of T and B lymphocytes in vitro. II. Biological and biochemical properties of an allogeneic effect factor (AEF) active in triggering specific B lymphocytes. J Exp Med. 1974 Jul 1;140(1):19–37. doi: 10.1084/jem.140.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armerding D., Kubo R. T., Grey H. M., Katz D. H. Activation of T and B lymphocytes in vitro: presence of beta2-microblobulin determinants on allogeneic effect factor. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4577–4581. doi: 10.1073/pnas.72.11.4577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Armerding D., Sachs D. H., Katz D. H. Activation of T and B lymphocytes in vitro. III. Presence of Ia determinants on allogeneic effect factor. J Exp Med. 1974 Dec 1;140(6):1717–1722. doi: 10.1084/jem.140.6.1717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bechtol K. B., McDevitt H. O. Antibody response of C3H in equilibrium (CKB X CWB)F1 tetraparental mice to poly-L(Tyr,Glu)-poly-D,L-Ala-poly-L-Lys immunization. J Exp Med. 1976 Jul 1;144(1):123–144. doi: 10.1084/jem.144.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
  6. Click R. E., Benck L., Alter B. J. Immune responses in vitro. I. Culture conditions for antibody synthesis. Cell Immunol. 1972 Feb;3(2):264–276. doi: 10.1016/0008-8749(72)90165-7. [DOI] [PubMed] [Google Scholar]
  7. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  8. Erb P., Feldmann M. The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. J Exp Med. 1975 Aug 1;142(2):460–472. doi: 10.1084/jem.142.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Frelinger J. A., Neiderhuber J. E., David C. S., Shreffler D. C. Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes. J Exp Med. 1974 Nov 1;140(5):1273–1284. doi: 10.1084/jem.140.5.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grumet F. C. Genetic control of the immune response. A selective defect in immunologic (IgG) memory in nonresponder mice. J Exp Med. 1972 Jan;135(1):110–125. doi: 10.1084/jem.135.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Heber-Katz E., Wilson D. B. Collaboration of allogeneic T and B lymphocytes in the primary antibody response to sheep erythrocytes in vitro. J Exp Med. 1975 Oct 1;142(4):928–935. doi: 10.1084/jem.142.4.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lonai P., McDevitt H. O. I-region genes are expressed on T and B lymphocytes. Studies of the mixed lymphocyte reaction (MLR). J Exp Med. 1974 Nov 1;140(5):1317–1323. doi: 10.1084/jem.140.5.1317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McDevitt H. O., Delovitch T. L., Press J. L. Functional and genetic analysis of Ia antigens. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):489–496. doi: 10.1101/sqb.1977.041.01.056. [DOI] [PubMed] [Google Scholar]
  14. McDevitt H. O., Delovitch T. L., Press J. L., Murphy D. B. Genetic and functional analysis of the Ia antigens: their possible role in regulating the immune response. Transplant Rev. 1976;30:197–235. doi: 10.1111/j.1600-065x.1976.tb00221.x. [DOI] [PubMed] [Google Scholar]
  15. Nathenson S. G., Cullen S. E. Biochemical properties and immunochemical-genetic relationships of mouse H-2 alloantigens. Biochim Biophys Acta. 1974 Apr 8;344(1):1–25. doi: 10.1016/0304-4157(74)90006-9. [DOI] [PubMed] [Google Scholar]
  16. Okumura K., Herzenberg L. A., Murphy D. B., McDevitt H. O., Herzenberg L. A. Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):685–698. doi: 10.1084/jem.144.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ordal J. C., Grumet F. C. Genetic control of the immune response. The effect of graft-versus-host reaction on the antibody response to poly-L(Tyr, Glu)-poly-D,L-Ala--poly-L-Lys in nonresponder mice. J Exp Med. 1972 Nov 1;136(5):1195–1206. doi: 10.1084/jem.136.5.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pierce S. K., Klinman N. R. The allogeneic bisection of carrier-specific enhancement of monoclonal B-cell responses. J Exp Med. 1975 Nov 1;142(5):1165–1179. doi: 10.1084/jem.142.5.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Press J. L., Klinman N. R., McDevitt H. O. Expression of Ia antigens on hapten-specific B cells. I. Delineation of B-cell subpopulations. J Exp Med. 1976 Aug 1;144(2):414–427. doi: 10.1084/jem.144.2.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rich S. S., Rich R. R. Regulatory mechanisms in cell-mediated immune responses. III. I-region control of suppressor cell interaction with responder cells in mixed lymphocyte reactions. J Exp Med. 1976 Mar 1;143(3):672–677. doi: 10.1084/jem.143.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  22. Tada T., Taniguchi M., David C. S. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. doi: 10.1084/jem.144.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. von Boehmer H., Hudson L., Sprent J. Collaboration of histoincompatible T and B lymphocytes using cells from tetraparental bone marrow chimeras. J Exp Med. 1975 Oct 1;142(4):989–997. doi: 10.1084/jem.142.4.989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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