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. 1979 Jun;138(3):691–698. doi: 10.1128/jb.138.3.691-698.1979

Lactose metabolism involving phospho-beta-galactosidase in Klebsiella.

B G Hall
PMCID: PMC218092  PMID: 110764

Abstract

Klebsiella strain RE1755A is a Lac- Gal- mutant which has lost both of its lac operons, but possesses a gene specifying beta-galactosidase III, an enzyme which hydrolyzes o-nitrophenyl-beta-D-galactopyranoside but does not hydrolyze lactose. Selective pressure was applied to isolate mutants able to utilize lactose. The lactose-utilizing mutants obtained were shown to possess an unaltered beta-galactosidase III. Lactose utilization was shown to result from a pleiotropic mutation which also (i) permits galactose utilization and (ii) prevents induction of beta-galactosidase III synthesis by lactose. Evidence is presented suggesting that a phospho-beta-galactosidase enzyme is involved in lactose metabolism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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