Abstract
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARM W. Mutants of phage T4 with increased sensitivity to ultraviolet. Virology. 1963 Jan;19:66–71. doi: 10.1016/0042-6822(63)90025-4. [DOI] [PubMed] [Google Scholar]
- HOWARD-FLANDERS P., THERIOT L. A method for selecting radiation-sensitive mutants of Escherichia coli. Genetics. 1962 Sep;47:1219–1224. doi: 10.1093/genetics/47.9.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HURWITZ C., REINER J. M., LANDAU J. V. Studies in the physiology and biochemistry of penicillin-induced spheroplasts of Escherichia coli. J Bacteriol. 1958 Dec;76(6):612–617. doi: 10.1128/jb.76.6.612-617.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamkalo B. A., Swenson P. A. Effects of ultraviolet radiation on respiration and growth in radiation-resistant and radiation-sensitive strains of Escherichia coli B. J Bacteriol. 1969 Sep;99(3):815–823. doi: 10.1128/jb.99.3.815-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JAGGER J. A small and inexpensive ultraviolet dose-rate meter useful in biological experiements. Radiat Res. 1961 Apr;14:394–403. [PubMed] [Google Scholar]
- KELNER A. CORRELATION BETWEEN GENETIC TRANSFORMABILITY AND NON-PHOTOREACTIVABILITY IN BACILLUS SUBTILIS. J Bacteriol. 1964 Jun;87:1295–1303. doi: 10.1128/jb.87.6.1295-1303.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELNER A. Growth, respiration, and nucleic acid synthesis in ultraviolet-irradiated and in photoreactivated Escherichia coli. J Bacteriol. 1953 Mar;65(3):252–262. doi: 10.1128/jb.65.3.252-262.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kontomichalou P., Mitani M., Clowes R. C. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. J Bacteriol. 1970 Oct;104(1):34–44. doi: 10.1128/jb.104.1.34-44.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEDERBERG J. Mechanism of action of penicillin. J Bacteriol. 1957 Jan;73(1):144–144. doi: 10.1128/jb.73.1.144-144.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
- Schwarz U., Asmus A., Frank H. Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969 May 14;41(3):419–429. doi: 10.1016/0022-2836(69)90285-x. [DOI] [PubMed] [Google Scholar]
- Takebe H., Jagger J. Action spectrum for growth delay induced in Escherichia coli B-r by far-ultraviolet radiation. J Bacteriol. 1969 May;98(2):677–682. doi: 10.1128/jb.98.2.677-682.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]