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. 1979 Jun;138(3):783–787. doi: 10.1128/jb.138.3.783-787.1979

Genetic mapping of a mutation affecting pyridine nucleotide transhydrogenase in Escherichia coli.

R L Hanson, C Rose
PMCID: PMC218105  PMID: 378938

Abstract

A mutation, pnt-1, causing loss of pyridine nucleotide transhydrogenase activity in Escherichia coli, was mapped by assaying for the enzyme in extracts of recombinant strains produced by conjugation, F-duction, and P1 transduction. The site of this mutation was near min 35, counterclockwise from man, and it co-transduced 59% with man. The mutation was associated with loss from the cell membrane fraction of energy-independent and adenosine 5'-triphosphate-dependent transhydrogenase activities, but reduced nicotinamide adenine dinucleotide dehydrogenase activity was not affected. Strains were constructed which lack phosphoglucoisomerase (pgi-2) and which carry either pnt+ or pnt-1. Although such strains, when grown on glucose, are expected to produce a large excess of reduced nicotinamide adenine dinucleotide phosphate, the growth rate was not affected by the pnt-1 allele.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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