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. 1979 Jun;138(3):853–860. doi: 10.1128/jb.138.3.853-860.1979

Yeast cytochrome c-specific protein-lysine methyltransferase: coordinate regulation with cytochrome c and activities in cyc mutants.

H N Liao, F Sherman
PMCID: PMC218114  PMID: 222727

Abstract

The cytochromes c of fungi and higher plants contain one or two residues of epsilon-N-trimethyllysine, whose biological role is unknown. A cytochrome c-specific S-adenosylmethionine:protein-sysine methyltransferase (methylase) activity was shown to be present in extracts of the bakers' yeast Saccharomyces cerevisiae, and basic kinetic properties of this enzyme are described. The specific activity of the methylase was lower in extracts of cells grown under conditions of catabolite (glucose) repression or anaerobiosis where cytochrome c levels were low, compared with cells grown under derepressed conditions where cytochrome c levels were high. During anaerobic-to-aerobic adaptation, the methylase was induced in parallel with cytochrome c, thus suggesting that the syntheses of cytochrome c and cytochrome c methylase are coordinately regulated. None of the cyc strains surveyed (cyc1, cyc2, cyc3, cyc4, cyc5, and cyc6) had diminished levels of methylase, although some of them were completely or almost completely deficient in cytochrome c.

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Selected References

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  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. Cantoni G. L. Biological methylation: selected aspects. Annu Rev Biochem. 1975;44:435–451. doi: 10.1146/annurev.bi.44.070175.002251. [DOI] [PubMed] [Google Scholar]
  3. Criddle R. S., Schatz G. Promitochondria of anaerobically grown yeast. I. Isolation and biochemical properties. Biochemistry. 1969 Jan;8(1):322–334. doi: 10.1021/bi00829a045. [DOI] [PubMed] [Google Scholar]
  4. DeLange R. J., Glazer A. N., Smith E. L. Identification and location of episilon-N-trimethyllysine in yeast cytochromes c. J Biol Chem. 1970 Jul 10;245(13):3325–3327. [PubMed] [Google Scholar]
  5. DeLange R. J., Glazer A. N., Smith E. L. Presence and location of an unusual amino acid, epsilon-N-trimethyllysine, in cytochrome c of wheat germ and Neurospora. J Biol Chem. 1969 Mar 10;244(5):1385–1388. [PubMed] [Google Scholar]
  6. Downie J. A., Stewart J. W., Sherman F. Yeast mutants defective in iso-2-cytochrome c. J Mol Biol. 1977 Dec 5;117(2):369–386. doi: 10.1016/0022-2836(77)90133-4. [DOI] [PubMed] [Google Scholar]
  7. Durban E., Nochumson S., Kim S., Paik W. K., Chan S. K. Cytochrome c-specific protein-lysine methyltransferase from Neurospora crassa. Purification, characterization, and substrate requirements. J Biol Chem. 1978 Mar 10;253(5):1427–1435. [PubMed] [Google Scholar]
  8. EPHRUSSI B., SLONIMSKI P. P., PERRODIN G. La synthèse adaptative des cytochromes chez la levure de boulangerie. Biochim Biophys Acta. 1950 Nov;6(2):256–267. doi: 10.1016/0006-3002(50)90098-9. [DOI] [PubMed] [Google Scholar]
  9. Foucher M., Verdière J., Lederer F., Slonimski P. P. On the presence of a non-trimethylated iso-1 cytochrome c in a wild-type strain of Saccharomyces cerevisiae. Eur J Biochem. 1972 Nov 21;31(1):139–143. doi: 10.1111/j.1432-1033.1972.tb02511.x. [DOI] [PubMed] [Google Scholar]
  10. Looze Y., Polastro E., Gielens C., Léonis J. Iso-cytochrome c species from baker's yeast. Analysis of their circular-dichroism spectra. Biochem J. 1976 Sep 1;157(3):773–775. doi: 10.1042/bj1570773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nochumson S., Durban E., Kim S., Paik W. K. Cytochrome c-specific protein methylase III from Neurospora crassa. Biochem J. 1977 Jul 1;165(1):11–18. doi: 10.1042/bj1650011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Paik W. K., Kim S. Protein methylation: chemical, enzymological, and biological significance. Adv Enzymol Relat Areas Mol Biol. 1975;42:227–286. doi: 10.1002/9780470122877.ch5. [DOI] [PubMed] [Google Scholar]
  13. Polastro E. T., Deconinck M. M., Devogel M. R., Mailier E. L., Looze Y. R., Schnek A. G., Leonis J. Evidence that trimethylation of iso-I-cytochrome c from Saccharomyces cerevisiae affects interaction with the mitochondrion. FEBS Lett. 1978 Feb 1;86(1):17–20. doi: 10.1016/0014-5793(78)80088-x. [DOI] [PubMed] [Google Scholar]
  14. Polastro E., Looze Y., Léonis J. Study of the biological significance of cytochrome methylation. I. Thermal, acid and guanidinium hydrochloride denaturations of baker's yeast ferricytochromes c. Biochim Biophys Acta. 1976 Sep 28;446(1):310–320. doi: 10.1016/0005-2795(76)90121-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rouslin W. Oxygen dependence of promitochondrial and cytoplasmic protein synthesis in the formation of electron transfer complexes III and IV in adapting Bakers' yeast. Arch Biochem Biophys. 1975 Jun;168(2):685–692. doi: 10.1016/0003-9861(75)90302-1. [DOI] [PubMed] [Google Scholar]
  16. SHERMAN F. MUTANTS OF YEAST DEFICIENT IN CYTOCHROME C. Genetics. 1964 Jan;49:39–48. doi: 10.1093/genetics/49.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  18. Sanders H. K., Mied P. A., Briquet M., Hernandez-Rodriguez J., Gottal R. F., Mattoon J. R. Regulation of mitochondrial biogenesis: yeast mutants deficient in synthesis of delta-aminolevulinic acid. J Mol Biol. 1973 Oct 15;80(1):17–39. doi: 10.1016/0022-2836(73)90231-3. [DOI] [PubMed] [Google Scholar]
  19. Scott W. A., Mitchell H. K. Secondary modification of cytochrome c by Neurospora crassa. Biochemistry. 1969 Nov;8(11):4282–4289. doi: 10.1021/bi00839a009. [DOI] [PubMed] [Google Scholar]
  20. Sherman F., Stewart J. W., Jackson M., Gilmore R. A., Parker J. H. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. doi: 10.1093/genetics/77.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sherman F., Stewart J. W., Parker J. H., Inhaber E., Shipman N. A., Putterman G. J., Gardisky R. L., Margoliash E. The mutational alteration of the primary structure of yeast iso-1-cytochrome c. J Biol Chem. 1968 Oct 25;243(20):5446–5456. [PubMed] [Google Scholar]
  22. Sherman F., Taber H., Campbell W. Genetic determination of iso-cytochromes c in yeast. J Mol Biol. 1965 Aug;13(1):21–39. doi: 10.1016/s0022-2836(65)80077-8. [DOI] [PubMed] [Google Scholar]
  23. Woods R. A., Sanders H. K., Briquet M., Foury F., Drysdale B. E., Mattoon J. R. Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring delta-aminolevulinic acid. J Biol Chem. 1975 Dec 10;250(23):9090–9098. [PubMed] [Google Scholar]

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