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. 1979 May;138(2):320–323. doi: 10.1128/jb.138.2.320-323.1979

Metabolism of pyrimidine bases and nucleosides in Neisseria meningitidis.

S Jyssum, K Jyssum
PMCID: PMC218180  PMID: 108255

Abstract

In Neisseria meningitidis, uridine, deoxyuridine, cytosine, cytidine, or deoxycytidine could not be used by uracil-requiring mutants as pyrimidine sources. Consistent with these findings, only 5-fluorouracil of the different fluoropyrimidine bases and nucleosides showed any inhibitory effect on the growth of four prototrophic strains of N. meningitidis. Likewise, only radioactive uracil was readily incorporated into nucleic acids, whereas uptake of radioactive uridine, cytosine, or cytidine could not be demonstrated. Uracil was converted to uridine 5'-monophosphate by uracil phosphoribosyltransferase, whereas enzyme activities for conversion of cytosine or any of the nucleosides were not detectable in meningococcal extracts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRESNICK E., SINGER S., HITCHINGS G. H. Mechanism of action of 6-azacytosine in bacteria. Biochim Biophys Acta. 1960 Jan 15;37:251–257. doi: 10.1016/0006-3002(60)90231-6. [DOI] [PubMed] [Google Scholar]
  2. Beck C. F., Ingraham J. L., Neuhard J., Thomassen E. Metabolism of pyrimidines and pyrimidine nucleosides by Salmonella typhimurium. J Bacteriol. 1972 Apr;110(1):219–228. doi: 10.1128/jb.110.1.219-228.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hammer-Jespersen K., Munch-Petersen A., Schwartz M., Nygaard P. Induction of enzymes involed in the catabolism of deoxyribonucleosides and ribonucleosides in Escherichia coli K 12. Eur J Biochem. 1971 Apr 30;19(4):533–538. doi: 10.1111/j.1432-1033.1971.tb01345.x. [DOI] [PubMed] [Google Scholar]
  4. Holten E., Vaage L., Neess C., Midtvedt T., Jyssum K. Sulphonamide-resistant meningococci after sulphonamide prophylaxis among vaval recruits in Norway. Scand J Infect Dis. 1969;1(3):185–189. [PubMed] [Google Scholar]
  5. JYSSUM K. Assimilation of nitrogen in meningococci grown with the ammonium ion as sole nitrogen source. Acta Pathol Microbiol Scand. 1959;46:320–332. [PubMed] [Google Scholar]
  6. Jyssum K. Origin and sequence of chromosome replication in Neisseria meningitidis: influence of a genetic factor determining competence. J Bacteriol. 1969 Sep;99(3):757–763. doi: 10.1128/jb.99.3.757-763.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jyssum S., Bovre K. Search for thymidine phosphorylase, nucleoside deoxyribosyltransferase and thymidine kinase in Moraxella, Acinetobacter, and allied bacteria. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Feb;82(1):57–66. doi: 10.1111/j.1699-0463.1974.tb02293.x. [DOI] [PubMed] [Google Scholar]
  8. Jyssum S. Labelling of DNA and RNA from thymine and thymidine in Neisseria meningitidis. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(2):325–334. doi: 10.1111/j.1699-0463.1972.tb00166.x. [DOI] [PubMed] [Google Scholar]
  9. Jyssum S. Purine metabolism in Neisseria meningitidis. 1. Utilization of exogenous adenine. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):508–520. doi: 10.1111/j.1699-0463.1974.tb02359.x. [DOI] [PubMed] [Google Scholar]
  10. Jyssum S. Purine metabolism in Neisseria meningitidis. 2. Utilization of exogenous adenosine, guanosine and inosine. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):885–894. [PubMed] [Google Scholar]
  11. Jyssum S. Utilization of thymine, thymidine and TMP by Neisseria meningitidis. 2. Lack of enzymes for specific incorporation of exogenous thymine, thymidine and TMP into DNA. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(6):778–788. doi: 10.1111/j.1699-0463.1971.tb00111.x. [DOI] [PubMed] [Google Scholar]
  12. Karlström H. O. Inability of Escherichia coli B to incorporate added deoxycytidine, deoxyandenosine, and deoxyguanosine into DNA. Eur J Biochem. 1970 Nov;17(1):68–71. doi: 10.1111/j.1432-1033.1970.tb01135.x. [DOI] [PubMed] [Google Scholar]
  13. Kingsbury D. T., Duncan J. F. Use of exogenous adenine to label the nucleic acids of wild-type Neisseria meningitidis. J Bacteriol. 1967 Oct;94(4):1262–1263. doi: 10.1128/jb.94.4.1262-1263.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LIE S. PRODUCTION OF AUXOTROPHIC MUTANTS OF NEISSERIA MENINGITIDIS BY NITROUS ACID. Acta Pathol Microbiol Scand. 1965;63:615–622. doi: 10.1111/apm.1965.63.4.615. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Mitchell A., Finch L. R. Pathways of nucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1977 Jun;130(3):1047–1054. doi: 10.1128/jb.130.3.1047-1054.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Molloy A., Finch L. R. Uridine-5'-monophosphate pyrophosphorylase activity from Escherichia coli. FEBS Lett. 1969 Nov 12;5(3):211–213. doi: 10.1016/0014-5793(69)80334-0. [DOI] [PubMed] [Google Scholar]
  19. Munch-Petersen A., Mygind B. Nucleoside transport systems in Escherichia coli K12: specificity and regulation. J Cell Physiol. 1976 Dec;89(4):551–559. doi: 10.1002/jcp.1040890410. [DOI] [PubMed] [Google Scholar]
  20. O'Donovan G. A., Neuhard J. Pyrimidine metabolism in microorganisms. Bacteriol Rev. 1970 Sep;34(3):278–343. doi: 10.1128/br.34.3.278-343.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OKAZAKI R., KORNBERG A. DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME. J Biol Chem. 1964 Jan;239:269–274. [PubMed] [Google Scholar]
  22. Ovrebo S., Kleppe K. Pyrimidine metabolism in Acinetobacter calcoaceticus. J Bacteriol. 1973 Oct;116(1):331–336. doi: 10.1128/jb.116.1.331-336.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rima B. K., Takahashi I. Metabolism of pyrimidine bases and nucleosides in Bacillus subtilis. J Bacteriol. 1977 Feb;129(2):574–579. doi: 10.1128/jb.129.2.574-579.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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