Abstract
The state in which macrophages (Mphi) from regressing Moloney sarcomas could kill tumor target cells was a highly labile one which decayed rapidly in vitro. Thereafter, regressor Mphi were noncytolytic. Mphi from several different progressing sarcomas failed to kill, even when challenged with target cells immediately after explantation. Similarly, thioglycollate-induced peritoneal Mphi (TG-Mphi) did not kill. Noncytolygic Mphi derived either from progressing sarcomas or from long- term (up to 96 h) cultures of regressor Mphi were exquisitely sensitive to stimulation by bacterial lipopolysaccharide (LPS); picogram/milliliter amounts induced killing. Similar concentrations of LPS had no demonstrable effect on TG-Mphi. Thus, tumor Mphi generally appeared to have been primed in vivo, with those in regressing sarcomas having additionally acquired cytolytic activity. Inability of progressor Mphi to kill apparently stemmed from lack of, or failure to respond to, the signal needed in vivo to trigger cytolytic activity, rather than the total absence of activation.
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Selected References
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- Alexander P., Evans R. Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971 Jul 21;232(29):76–78. doi: 10.1038/newbio232076a0. [DOI] [PubMed] [Google Scholar]
- CYNKIN M. A., ASHWELL G. Estimation of 3-deoxy sugars by means of the malonaldehyde-thiobarbituric acid reaction. Nature. 1960 Apr 9;186:155–156. doi: 10.1038/186155a0. [DOI] [PubMed] [Google Scholar]
- Chapman H. A., Jr, Hibbs J. B., Jr Modulation of macrophage tumoricidal capability by components of normal serum: a central role for lipid. Science. 1977 Jul 15;197(4300):282–285. doi: 10.1126/science.195338. [DOI] [PubMed] [Google Scholar]
- Evans R. Macrophages and the tumour bearing host. Br J Cancer Suppl. 1973 Aug;1:19–25. [PMC free article] [PubMed] [Google Scholar]
- Gillespie G. Y., Hansen C. B., Hoskins R. G., Russell S. W. Inflammatory cells in solid murine neoplasms. IV. Cytolytic T lymphocytes isolated from regressing or progressing Moloney sarcomas. J Immunol. 1977 Aug;119(2):564–570. [PubMed] [Google Scholar]
- Hibbs J. B., Jr, Taintor R. R., Chapman H. A., Jr, Weinberg J. B. Macrophage tumor killing: influence of the local environment. Science. 1977 Jul 15;197(4300):279–282. doi: 10.1126/science.327547. [DOI] [PubMed] [Google Scholar]
- Massicot J. G., Woods W. A., Chirigos M. A. Cell line derived from a murine sarcoma virus (Moloney Pseudotype)-induced tumor: cultural, antigenic, and virological properties. Appl Microbiol. 1971 Dec;22(6):1119–1122. doi: 10.1128/am.22.6.1119-1122.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrison D. C., Leive L. Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures. J Biol Chem. 1975 Apr 25;250(8):2911–2919. [PubMed] [Google Scholar]
- Piessens W. F., Churchill W. H., Jr, David Macrophages activated in vitro with lymphocyte mediators kill neoplastic but not normal cells. J Immunol. 1975 Jan;114(1 Pt 2):293–299. [PubMed] [Google Scholar]
- Russell S. W., Doe W. F., Hoskins R. G., Cochrane C. G. Inflammatory cells in solid murine neoplasms. I. Tumor disaggregation and identification of constituent inflammatory cells. Int J Cancer. 1976 Sep 15;18(3):322–330. doi: 10.1002/ijc.2910180309. [DOI] [PubMed] [Google Scholar]
- Russell S. W., Gillespie G. Y., Hansen C. B., Cochrane C. G. Inflammatory cells in solid murine neoplasms. II. Cell types found throughout the course of Moloney sarcoma regression or progression. Int J Cancer. 1976 Sep 15;18(3):331–338. doi: 10.1002/ijc.2910180310. [DOI] [PubMed] [Google Scholar]
- Russell S. W., Gillespie G. Y., McIntosh A. T. Inflammatory cells in solid murine neoplasms. III. Cytotoxicity mediated in vitro by macrophages recovered from disaggregated regressing Moloney sarcomas. J Immunol. 1977 May;118(5):1574–1579. [PubMed] [Google Scholar]
- Todaro G. J., Baron S. The role of interferon in the inhibition of SV40 transformation of mouse cell line 3T3. Proc Natl Acad Sci U S A. 1965 Sep;54(3):752–756. doi: 10.1073/pnas.54.3.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Loveren H., Den Otter W. Macrophages in solid tumors. I. Immunologically specific effector cells. J Natl Cancer Inst. 1974 Oct;53(4):1057–1060. doi: 10.1093/jnci/53.4.1057. [DOI] [PubMed] [Google Scholar]