Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1977 Dec 1;146(6):1500–1510. doi: 10.1084/jem.146.6.1500

The interaction of Herpes Simplex Virus with murine lymphocytes. I. Mitogenic properties of herpes simplex virus

PMCID: PMC2181902  PMID: 200698

Abstract

Herpes simplex virus (HSV) stimulates DNA synthesis in mouse spleen cultures prepared from normal, macrophage-depleted, and T-cell-depleted spleen cells, but not from thymocytes. In addition, a polyclonal antibody response is observed in HSV-infected spleen cultures. These findings indicate that the cells stimulated to undergo DNA synthesis after HSV infection appear to be the bone marrow-derived lymphocytes. The newly synthesized DNA is host cell and not of viral origin. Heat treatment and ultraviolet irradiation of HSV before addition to spleen cultures prevents the induction of DNA synthesis. We consider the use of this system as assay for the study of cell transformation by HSV and also for the study of host cell control of the expression of the viral genome.

Full Text

The Full Text of this article is available as a PDF (651.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyd A. L., Orme T. W. Transformation of mouse cells after infection with ultraviolet irradiation-inactivated herpes simplex virus type 2. Int J Cancer. 1975 Oct 15;16(4):526–538. doi: 10.1002/ijc.2910160403. [DOI] [PubMed] [Google Scholar]
  2. Darai G., Munk K. Human embryonic lung cells abortively infected with Herpes virus hominis type 2 show some properties of cell transformation. Nat New Biol. 1973 Feb 28;241(113):268–269. doi: 10.1038/newbio241268a0. [DOI] [PubMed] [Google Scholar]
  3. Davis D. B., Kingsbury D. T. Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus. J Virol. 1976 Mar;17(3):788–793. doi: 10.1128/jvi.17.3.788-793.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dowdle W. R., Nahmias A. J., Harwell R. W., Pauls F. P. Association of antigenic type of Herpesvirus hominis with site of viral recovery. J Immunol. 1967 Nov;99(5):974–980. [PubMed] [Google Scholar]
  5. Duff R., Rapp F. Oncogenic transformation of hamster cells after exposure to herpes simplex virus type 2. Nat New Biol. 1971 Sep 8;233(36):48–50. doi: 10.1038/newbio233048a0. [DOI] [PubMed] [Google Scholar]
  6. Duff R., Rapp F. Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. J Virol. 1973 Aug;12(2):209–217. doi: 10.1128/jvi.12.2.209-217.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duff R., Rapp F. Quantitative assay for transformation of 3T3 cells by herpes simplex virus type 2. J Virol. 1975 Mar;15(3):490–496. doi: 10.1128/jvi.15.3.490-496.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Epstein M. A. Aspects of the EB virus. Adv Cancer Res. 1970;13:383–411. doi: 10.1016/s0065-230x(08)60169-4. [DOI] [PubMed] [Google Scholar]
  9. Kimura S., Flannery V. L., Levy B., Schaffer P. A. Oncogenic transformation of primary hamster cells by herpes simplex virus type 2 (hsv-2) and an hsv-2 temperature-sensitive mutant. Int J Cancer. 1975 May 15;15(5):786–798. doi: 10.1002/ijc.2910150510. [DOI] [PubMed] [Google Scholar]
  10. Kirchner H., Hirt H. M., Kleinicke C., Munk K. Replication of herpes simplex virus in mouse spleen cell cultures stimulated by lipopolysaccharide. J Immunol. 1976 Nov;117(5 PT2):1753–1756. [PubMed] [Google Scholar]
  11. Kraiselburd E., Gage L. P., Weissbach A. Presence of a herpes simplex virus DNA fragment in an L cell clone obtained after infection with irradiated herpes simplex virus I. J Mol Biol. 1975 Oct 5;97(4):533–542. doi: 10.1016/s0022-2836(75)80057-x. [DOI] [PubMed] [Google Scholar]
  12. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  13. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nahmias A. J., Naib Z. M., Josey W. E., Murphy F. A., Luce C. F. Sarcomas after inoculation of newborn hamsters with Herpes virus hominis type 2 strains. Proc Soc Exp Biol Med. 1970 Sep;134(4):1065–1069. doi: 10.3181/00379727-134-34945. [DOI] [PubMed] [Google Scholar]
  15. Oldstone M. B., Haspel M. V., Pellegrino M. A., Kingsbury D. T., Olding L. Histocompatibility complex and virus infection latency and activation. Transplant Rev. 1976;31:225–239. doi: 10.1111/j.1600-065x.1976.tb01455.x. [DOI] [PubMed] [Google Scholar]
  16. Plummer G., Goodheart C. R., Miyagi M., Skinner G. R., Thouless M. E., Wildy P. Herpes simplex viruses: discrimination of types and correlation between different characteristics. Virology. 1974 Jul;60(1):206–216. doi: 10.1016/0042-6822(74)90378-x. [DOI] [PubMed] [Google Scholar]
  17. SVOBODA J. Presence of chicken tumour virus in the sarcoma of the adult rat inoculated after birth with Rous sarcoma tissue. Nature. 1960 Jun 18;186:980–981. doi: 10.1038/186980b0. [DOI] [PubMed] [Google Scholar]
  18. Sugino W. M., Kingsbury D. T. DNA homologies between strains of herpes simplex virus. Virology. 1976 Jun;71(2):605–608. doi: 10.1016/0042-6822(76)90387-1. [DOI] [PubMed] [Google Scholar]
  19. Watson J., Epstein R., Nakoinz I., Ralph P. The role of humoral factors in the initiation of in vitro primary immune responses. II. Effects of lymphocyte mitogens. J Immunol. 1973 Jan;110(1):43–52. [PubMed] [Google Scholar]
  20. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic respones to lipopolysaccharides. J Exp Med. 1974 Nov 1;140(5):1147–1161. doi: 10.1084/jem.140.5.1147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Watson J., Riblet R. Genetic control of responses to bacterial lipopolysaccharides in mice. II. A gene that influences a membrane component involved in the activation of bone marrow-derived lymphocytes by lipipolysaccharides. J Immunol. 1975 May;114(5):1462–1468. [PubMed] [Google Scholar]
  22. zur Hausen H. Oncogenic Herpes viruses. Biochim Biophys Acta. 1975 Mar 20;417(1):25–53. doi: 10.1016/0304-419x(75)90007-4. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES