Abstract
The development of IgG L-glutamic Acid60-L-alanine30-L-tyrosine10 (GAT)- specific plaque-forming cell responses in vitro by virgin and immune (responder X nonresponder)F1 spleen cells after stimulation with responder and nonresponder parental GAT-macrophages (Mphi) was investigated. Virgin F1 spleen cells developed comparable primary responses to both parental GAT-Mphi. By contrast, F1 spleen cells from mice immunized with GAT or responder parental GAT-Mphi developed secondary responses after stimulation with only responder parental GAT- Mphi. Spleen cells from F1 mice immunized with nonresponder parental GAT-Mphi developed secondary responses to these GAT-Mphi, but failed to respond to responder parental GAT-Mphi. These results are discussed in the context of genetic restrictions regulating Mphi-T-cell interactions in secondary antibody responses and the possible expression of Ir-gene function in Mphi.
Full Text
The Full Text of this article is available as a PDF (444.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Erb P., Feldmann M. The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens. J Exp Med. 1975 Aug 1;142(2):460–472. doi: 10.1084/jem.142.2.460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapp J. A., Pierce C. W., Benacerraf B. Genetic control of immune responses in vitro. I. Development of primary and secondary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro. J Exp Med. 1973 Nov 1;138(5):1107–1120. doi: 10.1084/jem.138.5.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapp J. A., Pierce C. W., Benacerraf B. Genetic control of immune responses in vitro. II. Cellular requirements for the development of primary plaque-forming cell responses to the random terpolymer 1-glutamic acid 60-1-alanine30-1-tyrosine10 (GAT) by mouse spleen cells in vitro. J Exp Med. 1973 Nov 1;138(5):1121–1132. doi: 10.1084/jem.138.5.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapp J. A., Pierce C. W., Benacerraf B. Genetic control of immune responses in vitro. VI. Experimental conditions for the development of helper T-cell activity specific for the terpolymer L-glutamic aicd60-L-alanine30-L-tyrosine10 (GAT) in nonresponder mice. J Exp Med. 1975 Jul 1;142(1):50–60. doi: 10.1084/jem.142.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kapp J. A., Pierce C. W., Schlossman S., Benacerraf B. Genetic control of immune responses in vitro. V. Stimulation of suppressor T cells in nonresponder mice by the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine 10 (GAT). J Exp Med. 1974 Sep 1;140(3):648–659. doi: 10.1084/jem.140.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Vadas M. A., Whitelaw A., Gamble J., Bernard C. Histocompatibility linked immune responsiveness and restrictions imposed on sensitized lymphocytes. J Exp Med. 1977 Jun 1;145(6):1623–1628. doi: 10.1084/jem.145.6.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Vadas M. A., Whitelaw A., Gamble J. Role of major histocompatibility complex gene products in delayed-type hypersensitivity. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2486–2490. doi: 10.1073/pnas.73.7.2486. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paul W. E., Shevach E. M., Pickeral S., Thomas D. W., Rosenthal A. S. Independent populations of primed F1 guinea pig T lymphocytes respond to antigen-pulsed parental peritoneal exudate cells. J Exp Med. 1977 Mar 1;145(3):618–630. doi: 10.1084/jem.145.3.618. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce C. W., Kapp J. A., Benacerraf B. Genetic restriction of macrophage-lymphocyte interactions in secondary antibody responses in vitro. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):563–570. doi: 10.1101/sqb.1977.041.01.065. [DOI] [PubMed] [Google Scholar]
- Pierce C. W., Kapp J. A., Benacerraf B. Regulation by the H-2 gene complex of macrophage-lymphoid cell interactions in secondary antibody responses in vitro. J Exp Med. 1976 Aug 1;144(2):371–381. doi: 10.1084/jem.144.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce C. W., Kapp J. A. Regulation of immune responses by suppressor T cells. Tohoku J Exp Med. 1976;5:91–143. [PubMed] [Google Scholar]
- Rosenthal A. S., Shevach E. M. Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes. J Exp Med. 1973 Nov 1;138(5):1194–1212. doi: 10.1084/jem.138.5.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shevach E. M., Rosenthal A. S. Function of macrophages in antigen recognition by guinea pig T lymphocytes. II. Role of the macrophage in the regulation of genetic control of the immune response. J Exp Med. 1973 Nov 1;138(5):1213–1229. doi: 10.1084/jem.138.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas D. W., Shevach E. M. Nature of the antigenic complex recognized by T lymphocytes: specific sensitization by antigens associated with allogeneic macrophages. Proc Natl Acad Sci U S A. 1977 May;74(5):2104–2108. doi: 10.1073/pnas.74.5.2104. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972;15:95–165. doi: 10.1016/s0065-2776(08)60684-7. [DOI] [PubMed] [Google Scholar]
- Yano A., Schwartz R. H., Paul W. E. Antigen presentation in the murine T-lymphocyte proliferative response. I. Requirement for genetic identity at the major histocompatibility complex. J Exp Med. 1977 Sep 1;146(3):828–843. doi: 10.1084/jem.146.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]