Abstract
The mechanism by which L-proline triggers germination in Bacillus megaterium QM B1551 spores was investigated. First, brief exposure of spores to L-proline, followed by dilution, was sufficient to trigger germination. Once germination was triggered, the spores continued initiation of germination and did not require high concentrations of L-proline. Triggering of germination was pH and temperature dependent. Second, enzymes for L-proline catabolism were absent in spores, and several non-metabolizable analogs of L-proline were effective trigger compounds. Third, triggering of germination occurred in the presence of inhibitors of proton motive force production, oxygen uptake, and metabolism. Fourth, uptake of L-proline occurred after the triggering of germination. These results argue that neither uptake nor metabolism of L-proline was necessary to trigger germination. Instead, L-proline probably causes a biophysical alteration in the spores that triggers the biochemical changes in germination.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. doi: 10.1146/annurev.bi.44.070175.002013. [DOI] [PubMed] [Google Scholar]
- Costilow R. N., Cooper D. Identity of proline dehydrogenase and delta1-pyrroline-5-carboxylic acid reductase in Clostridium sporogenes. J Bacteriol. 1978 Apr;134(1):139–146. doi: 10.1128/jb.134.1.139-146.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dendinger S., Brill W. J. Effect of the proline analogue baikiain on proline metabolism in Salmonella typhimurium. J Bacteriol. 1972 Dec;112(3):1134–1141. doi: 10.1128/jb.112.3.1134-1141.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dendinger S., Brill W. J. Regulation of proline degradation in Salmonella typhimurium. J Bacteriol. 1970 Jul;103(1):144–152. doi: 10.1128/jb.103.1.144-152.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dills S. S., Vary J. C. An evaluation of respiration chain-associated functions during initiation of germination of Bacillus megaterium spores. Biochim Biophys Acta. 1978 Jul 3;541(3):301–311. doi: 10.1016/0304-4165(78)90190-3. [DOI] [PubMed] [Google Scholar]
- FRANK L., RANHAND B. PROLINE METABOLISM IN ESCHERICHIA COLI. 3. THE PROLINE CATABOLIC PATHWAY. Arch Biochem Biophys. 1964 Aug;107:325–331. doi: 10.1016/0003-9861(64)90338-8. [DOI] [PubMed] [Google Scholar]
- HALMANN M., KEYNAN A. Stages in germination of spores of Bacillus licheniformis. J Bacteriol. 1962 Dec;84:1187–1193. doi: 10.1128/jb.84.6.1187-1193.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HARRELL W. K., HALVORSON H. Studies on the role of L-alanine in the germination of spores of Bacillus terminalis. J Bacteriol. 1955 Mar;69(3):275–279. doi: 10.1128/jb.69.3.275-279.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HYATT M. T., LEVINSON H. S. EFFECT OF SUGARS AND OTHER CARBON COMPOUNDS ON GERMINATION AND POSTGERMINATIVE DEVELOPMENT OF BACILLUS MEGATERIUM SPORES. J Bacteriol. 1964 Nov;88:1403–1415. doi: 10.1128/jb.88.5.1403-1415.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansford R. G., Sacktor B. The control of the oxidation of proline by isolated flight muscle mitochondria. J Biol Chem. 1970 Mar 10;245(5):991–994. [PubMed] [Google Scholar]
- Hazelbauer G. L., Adler J. Role of the galactose binding protein in chemotaxis of Escherichia coli toward galactose. Nat New Biol. 1971 Mar 24;230(12):101–104. doi: 10.1038/newbio230101a0. [DOI] [PubMed] [Google Scholar]
- Hsieh L. K., Vary J. C. Germination and peptidoglycan solubilization in Bacillus megaterium spores. J Bacteriol. 1975 Aug;123(2):463–470. doi: 10.1128/jb.123.2.463-470.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINSON H. S., HYATT M. T. Nitrogenous compounds in germination and postgerminative development of Bacillus megaterium spores. J Bacteriol. 1962 Jun;83:1224–1230. doi: 10.1128/jb.83.6.1224-1230.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laishley E. J., Bernlohr R. W. Regulation of arginine and proline catabolism in Bacillus licheniformis. J Bacteriol. 1968 Aug;96(2):322–329. doi: 10.1128/jb.96.2.322-329.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levinson H. S., Hyatt M. T. Sequence of events during Bacillus megaterim spore germination. J Bacteriol. 1966 May;91(5):1811–1818. doi: 10.1128/jb.91.5.1811-1818.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ordal G. W., Villani D. P., Nicholas R. A., Hamel F. G. Independence of proline chemotaxis and transport in Bacillus subtilis. J Biol Chem. 1978 Jul 25;253(14):4916–4919. [PubMed] [Google Scholar]
- Prasad C., Diesterhaft M., Freese E. Initiation of spore germination in glycolytic mutants of Bacillus subtilis. J Bacteriol. 1972 Apr;110(1):321–328. doi: 10.1128/jb.110.1.321-328.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rossignol D. P., Vary J. C. A unique method for studying the initiation of Bacillus megaterium spore germination. Biochem Biophys Res Commun. 1977 Dec 21;79(4):1098–1103. doi: 10.1016/0006-291x(77)91118-4. [DOI] [PubMed] [Google Scholar]
- Scott I. R., Ellar D. J. Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate. J Bacteriol. 1978 Jul;135(1):133–137. doi: 10.1128/jb.135.1.133-137.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow B., Setlow P. Levels of oxidized and reduced pyridine nucleotides in dormant spores and during growth, sporulation, and spore germination of Bacillus megaterium. J Bacteriol. 1977 Feb;129(2):857–865. doi: 10.1128/jb.129.2.857-865.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow P., Kornberg A. Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination. J Biol Chem. 1970 Jul 25;245(14):3645–3652. [PubMed] [Google Scholar]
- Setlow P., Primus G. Protein metabolism during germination of Bacillus megaterium spores. I. Protein synthesis and amino acid metabolism. J Biol Chem. 1975 Jan 25;250(2):623–630. [PubMed] [Google Scholar]
- Setlow P. Protein metabolism during germination of Bacillus megaterium spores. II. Degradation of pre-existing and newly synthesized protein. J Biol Chem. 1975 Jan 25;250(2):631–637. [PubMed] [Google Scholar]
- Seto B., Stadtman T. C. Purification and properties of proline reductase from Clostridium sticklandii. J Biol Chem. 1976 Apr 25;251(8):2435–2439. [PubMed] [Google Scholar]
- Shay L. K., Vary J. C. Biochemical studies on glucose initiated germination in Bacillus megaterium. Biochim Biophys Acta. 1978 Jan 18;538(2):284–292. doi: 10.1016/0304-4165(78)90356-2. [DOI] [PubMed] [Google Scholar]
- Van der Werf P., Orlowski M., Meister A. Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2982–2985. doi: 10.1073/pnas.68.12.2982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vary J. C. Spore germination of Bacillus megaterium QM B1551 mutants. J Bacteriol. 1972 Oct;112(1):640–642. doi: 10.1128/jb.112.1.640-642.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkinson B. J., Ellar D. J., Scott I. R., Koncewicz M. A. Rapid, chloramphenicol-resistant, activation of membrane electron transport on germination of Bacillus spores. Nature. 1977 Mar 10;266(5598):174–176. doi: 10.1038/266174a0. [DOI] [PubMed] [Google Scholar]
- Woese C. R., Vary J. C., Halvorson H. O. A kinetic model for bacterial spore germination. Proc Natl Acad Sci U S A. 1968 Mar;59(3):869–875. doi: 10.1073/pnas.59.3.869. [DOI] [PMC free article] [PubMed] [Google Scholar]