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. 1979 May;138(2):567–574. doi: 10.1128/jb.138.2.567-574.1979

Biochemical and topographical studies on Escherichia coli cell surface. IV. Giant spheroplast formation from a filamentous cell.

M O Onitsuka, Y Rikihisa, H B Maruyama
PMCID: PMC218214  PMID: 374397

Abstract

Long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from Escherichia coli surface mutant ONT-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. As distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the action of lysozyme in the presence of bovine serum albumin via the gradual fusion of distinct spheroplasting bulbs.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bhatti A. R., DeVoe I. W., Ingram J. M. Cell division in Pseudomonas aeruginosa: participation of alkaline phosphatase. J Bacteriol. 1976 Apr;126(1):400–409. doi: 10.1128/jb.126.1.400-409.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hirota Y., Mordoh J., Jacob F. On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol. 1970 Nov 14;53(3):369–387. doi: 10.1016/0022-2836(70)90072-0. [DOI] [PubMed] [Google Scholar]
  4. Hitchins A. D., Sadoff H. L. Properties of a thermosensitive asporogenous filamentous mutant of Bacillus megaterium. J Bacteriol. 1974 Jun;118(3):1167–1175. doi: 10.1128/jb.118.3.1167-1175.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hitchins A. D., Slepecky R. A. Antibiotic inhibition of the septation stage in sporulation of Bacillus megaterium. J Bacteriol. 1969 Mar;97(3):1513–1515. doi: 10.1128/jb.97.3.1513-1515.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kamiryo T., Strominger J. L. Penicillin-resistant temperature-sensitive mutants of Escherichia coli which synthesize hypo- or hyper-cross-linked peptidoglycan. J Bacteriol. 1974 Feb;117(2):568–577. doi: 10.1128/jb.117.2.568-577.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Maruyama H. B., Arisawa M., Ono-Onitsuka M. Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants. Infect Immun. 1975 Jun;11(6):1320–1324. doi: 10.1128/iai.11.6.1320-1324.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mendelson N. H., Haag S. M., Cole R. M. Cellular organization of Bacillus subtilis: sodium dodecyl sulfate-induced cell partitioning into zebra structures. J Bacteriol. 1976 Jun;126(3):1285–1296. doi: 10.1128/jb.126.3.1285-1296.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  11. OGG J. E., ZELLE M. R. Isolation and characterization of a large cell possibly polyploid strain of Escherichia coli. J Bacteriol. 1957 Oct;74(4):477–484. doi: 10.1128/jb.74.4.477-484.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ono-Onitsuka M., Maruyama H. B. Biochemical and topographical studies on the cell surface of Escherichia coli. I. Isolation and characterization of detergent-sensitive mutants. J Biochem. 1974 Sep;76(3):583–591. doi: 10.1093/oxfordjournals.jbchem.a130602. [DOI] [PubMed] [Google Scholar]
  13. Otsuji N., Iyehara H., Hideshima Y. Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation. J Bacteriol. 1974 Feb;117(2):337–344. doi: 10.1128/jb.117.2.337-344.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ron E. Z., Davis B. D. Growth rate of Escherichia coli at elevated temperatures: limitation by methionine. J Bacteriol. 1971 Aug;107(2):391–396. doi: 10.1128/jb.107.2.391-396.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saleh F., White P. J. Use of auxotrophic mutants to isolate LL- or DD-isomers of 2,6-diaminopimelic acid. J Gen Microbiol. 1976 Oct;96(2):253–261. doi: 10.1099/00221287-96-2-253. [DOI] [PubMed] [Google Scholar]
  16. Strominger J. L., Blumberg P. M., Suginaka H., Umbreit J., Wickus G. G. How penicillin kills bacteria: progress and problems. Proc R Soc Lond B Biol Sci. 1971 Dec 31;179(1057):369–383. doi: 10.1098/rspb.1971.0103. [DOI] [PubMed] [Google Scholar]
  17. ZELLE M. R., OGG J. E. Radiation resistance and genetic segregation in a large cell possibly polyploid strain of Escherichia coli. J Bacteriol. 1957 Oct;74(4):485–493. doi: 10.1128/jb.74.4.485-493.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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