Abstract
A lambdacI857 prophage inserted into one of the genes of the rha locus was used to select deletions unambiguously ordering the markers polA-glnA-rha-pfkA-tpi-metBJF. Transduction with phage P1 indicates at least 70% linkage between glnA and polA. The order of the pfk and tpi markers is reversed from that previously published. Despite the relatively large distance separating the glnA and rha loci, deletions removing this entire region have no obvious phenotype. The isolation of Tn10 transposons integrated at different sites between rha and glnA greatly facilitated this work.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson A., Cooper R. A. Genetic mapping of a locus for triosephosphate isomerase on the genome of Escherichia coli K12. J Gen Microbiol. 1970 Aug;62(3):329–334. doi: 10.1099/00221287-62-3-329. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bloom F. R., Levin M. S., Foor F., Tyler B. Regulation of glutamine synthetase formation in Escherichia coli: characterization of mutants lacking the uridylyltransferase. J Bacteriol. 1978 May;134(2):569–577. doi: 10.1128/jb.134.2.569-577.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G., Kotlarz D., Buc H. Two fructose 6-phosphate kinase activities in Escherichia coli. J Biol Chem. 1973 Jul 10;248(13):4865–4866. [PubMed] [Google Scholar]
- Kleckner N., Roth J., Botstein D. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977 Oct 15;116(1):125–159. doi: 10.1016/0022-2836(77)90123-1. [DOI] [PubMed] [Google Scholar]
- Lehman I. R., Uyemura D. G. DNA polymerase I: essential replication enzyme. Science. 1976 Sep 10;193(4257):963–969. doi: 10.1126/science.781842. [DOI] [PubMed] [Google Scholar]
- Morimyo M., Shimazu Y. Evidence that the gene uvrB is indispensable for a polymerase I deficient strain of Escherichia coli K-12. Mol Gen Genet. 1976 Sep 23;147(3):243–250. doi: 10.1007/BF00582875. [DOI] [PubMed] [Google Scholar]
- Nakamura Y., Yura T. Evidence for a positive regulation of RNA polymerase synthesis in Escherichia coli. J Mol Biol. 1975 Oct 5;97(4):621–642. doi: 10.1016/s0022-2836(75)80063-5. [DOI] [PubMed] [Google Scholar]
- Pahel G., Zelenetz A. D., Tyler B. M. gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J Bacteriol. 1978 Jan;133(1):139–148. doi: 10.1128/jb.133.1.139-148.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Power J. The L-rhamnose genetic system in Escherichia coli K-12. Genetics. 1967 Mar;55(3):557–568. doi: 10.1093/genetics/55.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
- Streicher S. L., Bender R. A., Magasanik B. Genetic control of glutamine synthetase in Klebiella aerogenes. J Bacteriol. 1975 Jan;121(1):320–331. doi: 10.1128/jb.121.1.320-331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vinopal R. T., Fraenkel D. G. PfkB and pfkC loci of Escherichia coli. J Bacteriol. 1975 Jun;122(3):1153–1161. doi: 10.1128/jb.122.3.1153-1161.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]