Abstract
Replicating molecules of the min-ColE1 plasmid pVH51 have been examined by electron microscopy after cleavage with the restriction endonuclease EcoRI. Replication apparently starts at a unique site indistinguishable from the origin of replication used by the parental plasmid ColE1. In contrast to ColE1, the structure of the majority of the replication intermediates was consistent with a bidirectional mode of replication. A minor portion of the molecules appeared to replicate unidirectionally in either direction from the same origin.
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Selected References
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- Cabello F., Timmis K., Cohen S. N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976 Jan 29;259(5541):285–290. doi: 10.1038/259285a0. [DOI] [PubMed] [Google Scholar]
- Crosa J. H., Luttropp L. K., Heffron F., Falkow S. Two replication initiation sites on R-plasmid DNA. Mol Gen Genet. 1975 Sep 15;140(1):39–50. doi: 10.1007/BF00268987. [DOI] [PubMed] [Google Scholar]
- Edlund T., Gustafsson P., Wolf-Watz H. Effect of thymine concentration on the mode of chromosomal replication in Escherichia coli K-12. J Mol Biol. 1976 Dec;108(2):295–303. doi: 10.1016/s0022-2836(76)80122-2. [DOI] [PubMed] [Google Scholar]
- Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hershfield V., Boyer H. W., Chow L., Helinski D. R. Characterization of a mini-ColC1 plasmid. J Bacteriol. 1976 Apr;126(1):447–453. doi: 10.1128/jb.126.1.447-453.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lovett M. A., Katz L., Helinski D. R. Unidirectional replication of plasmid ColE1 DNA. Nature. 1974 Sep 27;251(5473):337–340. doi: 10.1038/251337a0. [DOI] [PubMed] [Google Scholar]
- Lovett M. A., Sparks R. B., Helinski D. R. Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex. Proc Natl Acad Sci U S A. 1975 Aug;72(8):2905–2909. doi: 10.1073/pnas.72.8.2905. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer R. J., Helinski D. R. Unidirectional replication of the P-group plasmid RK2. Biochim Biophys Acta. 1977 Sep 6;478(1):109–113. doi: 10.1016/0005-2787(77)90249-0. [DOI] [PubMed] [Google Scholar]
- Schnös M., Inman R. B. Position of branch points in replicating lambda DNA. J Mol Biol. 1970 Jul 14;51(1):61–73. doi: 10.1016/0022-2836(70)90270-6. [DOI] [PubMed] [Google Scholar]
- Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]