Abstract
A set of lambda dilv phage have been used in a deletion mapping procedure to determine the location of two previously characterized ilvO alleles. In contrast to earlier conclusions derived from three-factor crosses and episome-shortening techniques with phage P1, the order found is ilvG-ilvO-ilvEDA. A three-factor cross with phage P1 is described that is not consistent with this location for an ilvO allele. Further analysis of this particular three-factor cross revealed than an artifact attributable to a mutual syntrophism had skewed the apparent frequency of inheritance of the ilvO locus. The role of mutual syntrophism is discussed as a source of mapping errors for the ilvO locus. The value of this set of lambda dilv phage and this mapping procedure for obtaining comparatively unambiguous data on the locations of the ilv structural and regulatory genes is demonstrated.
Full text
PDF








Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cohen B. M., Jones E. W. New Map Location of ilvO in ESCHERICHIA COLI. Genetics. 1976 Jun;83(2):201–225. doi: 10.1093/genetics/83.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Favre R., Wiater A., Puppo S., Iaccarino M. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12. Mol Gen Genet. 1976 Feb 2;143(3):243–252. doi: 10.1007/BF00269400. [DOI] [PubMed] [Google Scholar]
- Hahn J. E., Calhoun D. H. Suppressors of a genetic regulatory mutation affecting isoleucine-valine biosynthesis in Escherichia coli K-12. J Bacteriol. 1978 Oct;136(1):117–124. doi: 10.1128/jb.136.1.117-124.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kline E. L., Brown C. S., Coleman W. G., Jr, Umbarger H. E. Regulation of isoleucine-valine biosynthesis in an ilvDAC deletion strain of Escherichia coli K-12. Biochem Biophys Res Commun. 1974 Apr 23;57(4):1144–1151. doi: 10.1016/0006-291x(74)90816-x. [DOI] [PubMed] [Google Scholar]
- Levinthal M., Levinthal M., Williams L. S. The regulation of the ilv ADGE operon: evidence for positive control by threonine deaminase. J Mol Biol. 1976 Apr 15;102(3):453–465. doi: 10.1016/0022-2836(76)90327-2. [DOI] [PubMed] [Google Scholar]
- McCorkle G. M., Leathers T. D., Umbarger H. E. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc Natl Acad Sci U S A. 1978 Jan;75(1):89–93. doi: 10.1073/pnas.75.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGilvray D., Umbarger H. E. Regulation of transaminase C synthesis in Escherichia coli: conditional leucine auxotrophy. J Bacteriol. 1974 Nov;120(2):715–723. doi: 10.1128/jb.120.2.715-723.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pledger W. J., Umbarger H. E. Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity. J Bacteriol. 1973 Apr;114(1):195–207. doi: 10.1128/jb.114.1.195-207.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES. J Bacteriol. 1965 Mar;89:661–664. doi: 10.1128/jb.89.3.661-664.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION. J Bacteriol. 1964 Mar;87:566–573. doi: 10.1128/jb.87.3.566-573.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES. J Bacteriol. 1965 Mar;89:654–660. doi: 10.1128/jb.89.3.654-660.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith J. M., Smolin D. E., Umbarger H. E. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12. Mol Gen Genet. 1976 Oct 18;148(2):111–124. doi: 10.1007/BF00268374. [DOI] [PubMed] [Google Scholar]