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. 1979 Feb;137(2):795–801. doi: 10.1128/jb.137.2.795-801.1979

Analysis of corynomycolic acids and other fatty acids produced by Corynebacterium lepus grown on kerosene.

D G Cooper, J E Zajic, D E Gracey
PMCID: PMC218359  PMID: 422512

Abstract

The saponifiable carboxylic acids of the extracellular product of Corynebacterium lepus grown on kerosene have been isolated and characterized. About 25% of these acids were a mixture of simple, saturated fatty acids ranging from C13 to C24 and including both even and odd homologues. The distribution of these acids was bimodal, with maxima at C15 and C21. The other 75% of the acids was a mixture of corynomycolic acids [R1--CH(OH)--CH(R2)--COOH] ranging from C28 to C43. The R1 alkyl fragments varied from C16 to C25, and R2 fragments varied from C6 to C14. Both even and odd corynomycolic acid homologues were observed, and the distribution had a single pronounced maximum at C32 and C33. Bacterial utilization of the carboxylic oxidation products of the kerosene substrate is suggested to account for the wide distribution in chain length of these saturated fatty acids and for the observation of both even and odd homologues.

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Selected References

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  1. BENNET P., ASSELINEAU J. NATURE DES ACIDES GRAS DES GRAISSES DE LA SOUCHE BOVINE B.C.G. DE MYCOBACTERIUM TUBERCULOSIS. Bull Soc Chim Biol (Paris) 1963;45:1379–1393. [PubMed] [Google Scholar]
  2. Banis R. J., Peterson D. O., Bloch K. Mycobacterium smegmatis fatty acid synthetase. Polysaccharide stimulation of the rate-limiting step. J Biol Chem. 1977 Aug 25;252(16):5740–5744. [PubMed] [Google Scholar]
  3. Calderon M., Baumann W. J. Fractionation of neutral lipids on a lipophilic dextran gel. Biochim Biophys Acta. 1970 Jun 9;210(1):7–14. doi: 10.1016/0005-2760(70)90056-1. [DOI] [PubMed] [Google Scholar]
  4. Ingram L. O., Chevalier L. S., Gabba E. J., Ley K. D., Winters K. Propionate-induced synthesis of odd-chain-length fatty acids by Escherichia coli. J Bacteriol. 1977 Sep;131(3):1023–1025. doi: 10.1128/jb.131.3.1023-1025.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lynen F. Enzyme systems for fatty acid synthesis. Biochem Soc Symp. 1972;(35):5–26. [PubMed] [Google Scholar]
  6. Makula R. A., Finnerty W. R. Microbial assimilation of hydrocarbons: cellular distribution of fatty acids. J Bacteriol. 1972 Oct;112(1):398–407. doi: 10.1128/jb.112.1.398-407.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Makula R. A., Lockwood P. J., Finnerty W. R. Comparative analysis of the lipids of Acinetobacter species grown on hexadecane. J Bacteriol. 1975 Jan;121(1):250–258. doi: 10.1128/jb.121.1.250-258.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Makula R., Finnerty W. R. Microbial assimilation of hydrocarbons. I. Fatty acids derived from normal alkanes. J Bacteriol. 1968 Jun;95(6):2102–2107. doi: 10.1128/jb.95.6.2102-2107.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Makula R., Finnerty W. R. Microbial assimilation of hydrocarbons. II. Fatty acids derived from 1-alkenes. J Bacteriol. 1968 Jun;95(6):2108–2111. doi: 10.1128/jb.95.6.2108-2111.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Raymond R. L., Jamison V. W. Biochemical activities of Nocardia. Adv Appl Microbiol. 1971;14:93–122. doi: 10.1016/s0065-2164(08)70541-0. [DOI] [PubMed] [Google Scholar]
  11. STEWART J. E., KALLIO R. E. Bacterial hydrocarbon oxidation. II. Ester formation from alkanes. J Bacteriol. 1959 Nov;78:726–730. doi: 10.1128/jb.78.5.726-730.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STEWART J. E., KALLIO R. E., STEVENSON D. P., JONES A. C., SCHISSLER D. O. Bacterial hydrocarbon oxidation. I. Oxidation of n-hexadecane by a gram-negative coccus. J Bacteriol. 1959 Sep;78:441–448. doi: 10.1128/jb.78.3.441-448.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saito Y., Silvius J. R., McElhaney N. Membrane lipid biosynthesis in Acholeplasma laidlawii B: de novo biosynthesis of saturated fatty acids by growing cells. J Bacteriol. 1977 Nov;132(2):497–504. doi: 10.1128/jb.132.2.497-504.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Welby-Gieusse M., Lanéelle M. A., Asselineau J. Structure des acides corynomycoliqes de Corynebacterium hofmanii et leur implication biogénétique. Eur J Biochem. 1970 Mar 1;13(1):164–167. doi: 10.1111/j.1432-1033.1970.tb00913.x. [DOI] [PubMed] [Google Scholar]
  15. Zajic J. E., Guignard H., Gerson D. F. Emulsifying and surface active agents from Corynebacterium hydrocarboclastus. Biotechnol Bioeng. 1977 Sep;19(9):1285–1301. doi: 10.1002/bit.260190904. [DOI] [PubMed] [Google Scholar]

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