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. 1979 Feb;137(2):869–877. doi: 10.1128/jb.137.2.869-877.1979

Precursor-product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium.

R E Kessler, G D Shockman
PMCID: PMC218369  PMID: 106043

Abstract

Exponential biosynthesis and excretion of lipoteichoic acid (LTA) during the exponential phase of growth, and continued synthesis and excretion during valine starvation of Streptococcus faecium (S. faecalis ATCC 9790), were shown. During exponential growth, extracellular LTA (LTAx) accounted for approximately 13% of the total LTA in cultures, whereas during valine starvation, this percentage increased to approximately 60% within 4 h. LTAx was present in a low-molecular-weight, apparently deacylated form, whereas intracellular (LTAi) was present primarily in an apparently high-molecular-weight, acylated and micellar form. Experiments utilizing chases of either fully equilibrated or short pulses of [14C]- or [3H]glycerol were used to demonstrate that LTAx was derived directly from LTAi.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baddiley J. Teichoic acids in cell walls and membranes of bacteria. Essays Biochem. 1972;8:35–77. [PubMed] [Google Scholar]
  2. Beachey E. H., Ofek I. Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein. J Exp Med. 1976 Apr 1;143(4):759–771. doi: 10.1084/jem.143.4.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bleiweis A. S., Young F. E., Krause R. M. Cell walls of group D streptococci. II. Chemical studies on the type 1 antigen purified from the autolytic digest of cell walls. J Bacteriol. 1967 Nov;94(5):1381–1387. doi: 10.1128/jb.94.5.1381-1387.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boothby D., Daneo-Moore L., Higgins M. L., Coyette J., Shockman G. D. Turnover of bacterial cell wall peptidoglycans. J Biol Chem. 1973 Mar 25;248(6):2161–2169. [PubMed] [Google Scholar]
  5. Button D., Hemmings N. L. Lipoteichoic acid from Bacillus licheniformis 6346 MH-1. Comparative studies on the lipid portion of the lipoteichoic acid and the membrane glycolipid. Biochemistry. 1976 Mar 9;15(5):989–995. doi: 10.1021/bi00650a007. [DOI] [PubMed] [Google Scholar]
  6. Carson D., Daneo-Moore L. Effect of cerulenin on Streptococcus faecalis macromolecular synthesis and cell division. J Bacteriol. 1978 Feb;133(2):472–476. doi: 10.1128/jb.133.2.472-476.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cleveland R. F., Daneo-Moore L., Wicken A. J., Shockman G. D. Effect of lipoteichoic acid and lipids on lysis of intact cells of Streptococcus faecalis. J Bacteriol. 1976 Sep;127(3):1582–1584. doi: 10.1128/jb.127.3.1582-1584.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cleveland R. F., Holtje J. V., Wicken A. J., Tomasz A., Daneo-Moore L., Shockman G. D. Inhibition of bacterial wall lysins by lipoteichoic acids and related compounds. Biochem Biophys Res Commun. 1975 Dec 1;67(3):1128–1135. doi: 10.1016/0006-291x(75)90791-3. [DOI] [PubMed] [Google Scholar]
  9. Dezélée P., Shockman G. D. Studies of the formation of peptide cross-links in the cell wall peptidoglycan of Streptococcus faecalis. J Biol Chem. 1975 Sep 10;250(17):6806–6816. [PubMed] [Google Scholar]
  10. Emdur L. I., Chiu T. H. Turnover of phosphatidylglycerol in Streptococcus sanguis. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1137–1144. doi: 10.1016/s0006-291x(74)80097-5. [DOI] [PubMed] [Google Scholar]
  11. Ganfield M. C., Pieringer R. A. Phosphatidylkojibiosyl diglyceride. The covalently linked lipid constituent of the membrane lipoteichoic acid from Streptococcus faecalis (faecium) ATCC 9790. J Biol Chem. 1975 Jan 25;250(2):702–709. [PubMed] [Google Scholar]
  12. Glaser L., Lindsay B. The synthesis of lipoteichoic acid carrier. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1131–1136. doi: 10.1016/s0006-291x(74)80096-3. [DOI] [PubMed] [Google Scholar]
  13. Höltje J. V., Tomasz A. Lipoteichoic acid: a specific inhibitor of autolysin activity in Pneumococcus. Proc Natl Acad Sci U S A. 1975 May;72(5):1690–1694. doi: 10.1073/pnas.72.5.1690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Joseph R., Shockman G. D. Synthesis and excretion of glycerol teichoic acid during growth of two streptococcal species. Infect Immun. 1975 Aug;12(2):333–338. doi: 10.1128/iai.12.2.333-338.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Knox K. W., Wicken A. J. Immunological properties of teichoic acids. Bacteriol Rev. 1973 Jun;37(2):215–257. doi: 10.1128/br.37.2.215-257.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lambert P. A., Hancock I. C., Baddiley J. Occurrence and function of membrane teichoic acids. Biochim Biophys Acta. 1977 May 31;472(1):1–12. doi: 10.1016/0304-4157(77)90012-0. [DOI] [PubMed] [Google Scholar]
  17. Markham J. L., Knox K. W., Wicken A. J., Hewett M. J. Formation of extracellular lipoteichoic acid by oral streptococci and lactobacilli. Infect Immun. 1975 Aug;12(2):378–386. doi: 10.1128/iai.12.2.378-386.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roth G. S., Shockman G. D., Daneo-Moore L. Balanced macromolecular biosynthesis in "protoplasts" of Streptococcus faecalis. J Bacteriol. 1971 Mar;105(3):710–717. doi: 10.1128/jb.105.3.710-717.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SHOCKMAN G. D., KOLB J. J., TOENNIES G. Relations between bacterial cell wall synthesis, growth phase, and autolysis. J Biol Chem. 1958 Feb;230(2):961–977. [PubMed] [Google Scholar]
  20. Shockman G. D., Cheney M. C. Autolytic enzyme system of Streptococcus faecalis. V. Nature of the autolysin-cell wall complex and its relationship to properties of the autolytic enzyme of Streptococcus faecalis. J Bacteriol. 1969 Jun;98(3):1199–1207. doi: 10.1128/jb.98.3.1199-1207.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shockman G. D., Kessler R., Corentt J. B., Mychajlonka M. Turnover and excretion of streptococcal surface components. Adv Exp Med Biol. 1978;107:803–814. doi: 10.1007/978-1-4684-3369-2_90. [DOI] [PubMed] [Google Scholar]
  22. TOENNIES G., GALLANT D. L. The relation between photometric turbidity and bacterial concentration. Growth. 1949 Mar;13(1):7–20. [PubMed] [Google Scholar]
  23. Toennies G., Das D. N., Feng F. New observations on the determination of bacterial lipid phosphorus. Can J Microbiol. 1968 Apr;14(4):484–485. doi: 10.1139/m68-079. [DOI] [PubMed] [Google Scholar]
  24. Toon P., Brown P. E., Baddiley J. The lipid-teichoic acid complex in the cytoplasmic membrane of Streptococcus faecalis N.C.I.B. 8191. Biochem J. 1972 Apr;127(2):399–409. doi: 10.1042/bj1270399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WICKEN A. J., BADDILEY J. Structure of intracellular teichoic acids from group D streptococci. Biochem J. 1963 Apr;87:54–62. doi: 10.1042/bj0870054. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wicken A. J., Knox K. W. A serological comparison of the membrane teichoic acids from lactobacilli of different serological groups. J Gen Microbiol. 1971 Aug;67(2):251–254. doi: 10.1099/00221287-67-2-251. [DOI] [PubMed] [Google Scholar]
  27. Wicken A. J., Knox K. W. Lipoteichoic acids: a new class of bacterial antigen. Science. 1975 Mar 28;187(4182):1161–1167. doi: 10.1126/science.46620. [DOI] [PubMed] [Google Scholar]
  28. Wicken A. J., Knox K. W. Studies on the group F antigen of lactobacilli: isolation of a teichoic acid-lipid complex from Lactobacillus fermenti NCTC 6991. J Gen Microbiol. 1970 Mar;60(3):293–301. doi: 10.1099/00221287-60-3-293. [DOI] [PubMed] [Google Scholar]
  29. Wilkinson S. G. Glycosyl diglycerides from Pseudomonas rubescens. Biochim Biophys Acta. 1968 Oct 22;164(2):148–156. doi: 10.1016/0005-2760(68)90141-0. [DOI] [PubMed] [Google Scholar]
  30. Ziegler R. J., Daneo-Moore L. Effects of essential amino acid starvation in Streptococcus faecalis: structural change in the 50S ribosomal subunit. J Bacteriol. 1971 Jan;105(1):190–199. doi: 10.1128/jb.105.1.190-199.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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