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. 1979 Feb;137(2):1059–1062. doi: 10.1128/jb.137.2.1059-1062.1979

The relA locus specifies a positive effector in branched-chain amino acid transport regulation.

S C Quay, D L Oxender
PMCID: PMC218402  PMID: 370095

Abstract

The regulation of branched-chain amino acid transport and periplasmic binding proteins was studied in Escherichia coli strains which were isogenic except for the relA locus, the gene for the "stringent factor," which is responsible for guanosine tetraphosphate synthesis. The strain containing the relA mutation could not be derepressed for the synthesis of leucine transport or binding proteins when shifted from a medium containing all 20 amino acids in excess to one in which leucine was limiting. The relA+ strain showed normal derepression under these conditions.

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Selected References

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  1. Allaudeen H. S., Yang S. K., Söll D. Leucine tRNA(1) from HisT mutant of Salmonella typhimurium lacks two pseudouridines. FEBS Lett. 1972 Dec 1;28(2):205–208. doi: 10.1016/0014-5793(72)80713-0. [DOI] [PubMed] [Google Scholar]
  2. Anderson J. J., Quay S. C., Oxender D. L. Mapping of two loci affecting the regulation of branched-chain amino acid transport in Escherichia coli K-12. J Bacteriol. 1976 Apr;126(1):80–90. doi: 10.1128/jb.126.1.80-90.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bresalier R. S., Rizzino A. A., Freundlich M. Reduced maximal levels of derepression of the isoleucine-valine and leucine enzymes in hisT mutants of Salmonella typhimurium. Nature. 1975 Jan 24;253(5489):279–280. doi: 10.1038/253279a0. [DOI] [PubMed] [Google Scholar]
  4. Cashel M., Gallant J. Control of RNA synthesis in Escherichia coli. I. Amino acid dependence of the synthesis of the substrates of RNA polymerase. J Mol Biol. 1968 Jul 14;34(2):317–330. doi: 10.1016/0022-2836(68)90256-8. [DOI] [PubMed] [Google Scholar]
  5. Edlin G., Neuhard J. Regulation of nucleoside triphosphate pools in Escherichia coli. J Mol Biol. 1967 Mar 14;24(2):225–230. doi: 10.1016/0022-2836(67)90328-2. [DOI] [PubMed] [Google Scholar]
  6. Fraser J., Newman E. B. Derivation of glycine from threonine in Escherichia coli K-12 mutants. J Bacteriol. 1975 Jun;122(3):810–817. doi: 10.1128/jb.122.3.810-817.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Haseltine W. A., Block R. Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes. Proc Natl Acad Sci U S A. 1973 May;70(5):1564–1568. doi: 10.1073/pnas.70.5.1564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hölttä E., Jänne J., Pispa J. The regulation of polyamine synthesis during the stringent control in Escherichia coli. Biochem Biophys Res Commun. 1974 Aug 5;59(3):1104–1111. doi: 10.1016/s0006-291x(74)80092-6. [DOI] [PubMed] [Google Scholar]
  9. Kitchingman G. R., Fournier M. J. Modification-deficient transfer ribonucleic acids from relaxed control Escherichia coli: structures of the major undermodified phenylalanine and leucine transfer RNAs produced during leucine starvation. Biochemistry. 1977 May 17;16(10):2213–2220. doi: 10.1021/bi00629a027. [DOI] [PubMed] [Google Scholar]
  10. Kitchingman G. R., Webb E., Fournier M. J. Unique phenylalanine transfer ribonucleic acids in relaxed control Escherichia coli: genetic origin and some functional properties. Biochemistry. 1976 May 4;15(9):1848–1857. doi: 10.1021/bi00654a010. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lawther R. P., Hatfield W. Biochemical characterization of an Escherichia coli hisT strain. J Bacteriol. 1977 Apr;130(1):552–557. doi: 10.1128/jb.130.1.552-557.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morse D. E., Morse A. N. Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor. J Mol Biol. 1976 May 15;103(2):209–226. doi: 10.1016/0022-2836(76)90310-7. [DOI] [PubMed] [Google Scholar]
  14. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  15. Nierlich D. P. Amino acid control over RNA synthesis: a re-evaluation. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1345–1352. doi: 10.1073/pnas.60.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oxender D. L., Anderson J. J., Mayo M. M., Quay S. C. Leucine binding protein and regulation of transport in E. coli. J Supramol Struct. 1977;6(3):419–431. doi: 10.1002/jss.400060315. [DOI] [PubMed] [Google Scholar]
  17. Oxender D. L., Quay S. Binding proteins and membrane transport. Ann N Y Acad Sci. 1975 Dec 30;264:358–372. doi: 10.1111/j.1749-6632.1975.tb31496.x. [DOI] [PubMed] [Google Scholar]
  18. Quay S. C., Dick T. E., Oxender D. L. Role of transport systems in amino acid metabolism: leucine toxicity and the branched-chain amino acid transport systems. J Bacteriol. 1977 Mar;129(3):1257–1265. doi: 10.1128/jb.129.3.1257-1265.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Quay S. C., Kline E. L., Oxender D. L. Role of leucyl-tRNA synthetase in regulation of branched-chain amino-acid transport. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3921–3924. doi: 10.1073/pnas.72.10.3921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Quay S. C., Lawther R. P., Hatfield G. W., Oxender D. L. Branched-chain amino acid transport regulation in mutants blocked in tRNA maturation and transcriptional termination. J Bacteriol. 1978 May;134(2):683–686. doi: 10.1128/jb.134.2.683-686.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Quay S. C., Oxender D. L. Regulation of amino acid transport in Escherichia coli by transcription termination factor rho. J Bacteriol. 1977 Jun;130(3):1024–1029. doi: 10.1128/jb.130.3.1024-1029.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Quay S. C., Oxender D. L. Regulation of branched-chain amino acid transport in Escherichia coli. J Bacteriol. 1976 Sep;127(3):1225–1238. doi: 10.1128/jb.127.3.1225-1238.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Quay S. C., Oxender D. L., Tsuyumu S., Umbarger H. E. Separate regulation of transport and biosynthesis of leucine, isoleucine, and valine in bacteria. J Bacteriol. 1975 Jun;122(3):994–1000. doi: 10.1128/jb.122.3.994-1000.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Quay S., Christensen H. N. Basis of transport discrimination of arginine from other basic amino acids in Salmonella typhimurium. J Biol Chem. 1974 Nov 10;249(21):7011–7017. [PubMed] [Google Scholar]
  25. Rahmanian M., Claus D. R., Oxender D. L. Multiplicity of leucine transport systems in Escherichia coli K-12. J Bacteriol. 1973 Dec;116(3):1258–1266. doi: 10.1128/jb.116.3.1258-1266.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  27. Singer C. E., Smith G. R., Cortese R., Ames B. N. [Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications]. Nat New Biol. 1972 Jul 19;238(81):72–74. doi: 10.1038/newbio238072a0. [DOI] [PubMed] [Google Scholar]
  28. Smolin D. E., Umbarger H. E. Specificity of the stimulation of in vitro ribonucleic acid synthesis by guanosine 5'-diphosphate 3'-diphosphate. Mol Gen Genet. 1975 Dec 9;141(4):277–284. doi: 10.1007/BF00331449. [DOI] [PubMed] [Google Scholar]
  29. Sokawa Y., Nakao E., Kaziro Y. On the nature of the control by RC gene in e. coli: amino acid-dependent control of lipid synthesis. Biochem Biophys Res Commun. 1968 Oct 10;33(1):108–112. doi: 10.1016/0006-291x(68)90263-5. [DOI] [PubMed] [Google Scholar]
  30. Somerville C. R., Ahmed A. rel-dependent methionine requirement in revertants of a methionyl-transfer RNA synthetase mutant of Escherichia coli. J Mol Biol. 1977 Mar 25;111(1):77–81. doi: 10.1016/s0022-2836(77)80133-2. [DOI] [PubMed] [Google Scholar]
  31. Stephens J. C., Artz S. W., Ames B. N. Guanosine 5'-diphosphate 3'-diphosphate (ppGpp): positive effector for histidine operon transcription and general signal for amino-acid deficiency. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4389–4393. doi: 10.1073/pnas.72.11.4389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Travers A. RNA polymerase specificity and the control of growth. Nature. 1976 Oct 21;263(5579):641–646. doi: 10.1038/263641a0. [DOI] [PubMed] [Google Scholar]
  33. Travers A. Template selection by E. coli RNA polymerase holoenzyme. FEBS Lett. 1976 Oct 15;69(1):195–198. doi: 10.1016/0014-5793(76)80685-0. [DOI] [PubMed] [Google Scholar]

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