Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1978 Jan 1;147(1):87–97. doi: 10.1084/jem.147.1.87

Changes in suppressor mechanisms during postnatal development in mice

PMCID: PMC2184094  PMID: 304880

Abstract

The activity of suppressor cells from spleens of mice of varying ages was assessed by their addition to cultures of normal or SRBC immune spleen cells together with a challenge of SRBC. 1-wk and adult spleen cells were highly suppressive of the secondary in vitro antibody response to SRBC. 3-wk spleen cells were less active in suppressing this response. The nature of the suppression and the character of the suppressor cells changed in this period. Whereas adult spleen cells demonstrated specificity, 1-wk cells nonspecifically suppressed all responses tested. Further, unlike adult suppressor cells (which are Thy.1.2 positive), 1-wk suppressor cells are insensitive to anti- Thy.1.2 treatment in this system. Both cells are nonadherent to glass beads and nylon wool and are undetectable in the normal thymus.

Full Text

The Full Text of this article is available as a PDF (831.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker P. J., Reed N. D., Stashak P. W., Amsbaugh D. F., Prescott B. Regulation of the antibody response to type 3 pneumococcal polysaccharide. I. Nature of regulatory cells. J Exp Med. 1973 Jun 1;137(6):1431–1441. doi: 10.1084/jem.137.6.1431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beer A. E., Billingham R. E. Immunobiology of mammalian reproduction. Adv Immunol. 1971;14:1–84. doi: 10.1016/s0065-2776(08)60283-7. [DOI] [PubMed] [Google Scholar]
  3. Calkins C. E., Carboni J. M., Waksman B. H. B cells in the appendix and other lymphoid organs of the rabbit: complement receptor lymphocytes. J Immunol. 1975 Nov;115(5):1339–1345. [PubMed] [Google Scholar]
  4. Calkins C. E., Orbach-Arbouys S., Stutman O., Gershon R. K. Cell interactions in the suppression of in vitro antibody responses. J Exp Med. 1976 Jun 1;143(6):1421–1428. doi: 10.1084/jem.143.6.1421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Calkins C. E., Ozer H., Waksman B. H. B Cells in the appendix and other lymphoid organs of the rabbit: stimulation of DNA synthesis by anti-immunoglobulin. Cell Immunol. 1975 Jul;18(1):187–198. doi: 10.1016/0008-8749(75)90047-7. [DOI] [PubMed] [Google Scholar]
  6. Celada F. Quantitative studies of the adoptive immunological memory in mice. I. An age-dependent barrier to syngeneic transplantation. J Exp Med. 1966 Jul 1;124(1):1–14. doi: 10.1084/jem.124.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Durkin H. G., Bash J. A., Waksman B. H. Separation of T cell subpopulations capable of DNA synthesis, lymphotoxin release, and regulation of antigen and phytohemagglutinin responses on the basis of density and adherence properties. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5090–5094. doi: 10.1073/pnas.72.12.5090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dutton R. W. Suppressor T cells. Transplant Rev. 1975;26:39–55. doi: 10.1111/j.1600-065x.1975.tb00174.x. [DOI] [PubMed] [Google Scholar]
  9. Eardley D. D., Gershon R. K. Induction of specific suppressor T cells in vitro. J Immunol. 1976 Jul;117(1):313–318. [PubMed] [Google Scholar]
  10. Elson C. J., Taylor R. B. The suppressive effect of carrier priming on the response to a hapten-carrier conjugate. Eur J Immunol. 1974 Oct;4(10):682–687. doi: 10.1002/eji.1830041009. [DOI] [PubMed] [Google Scholar]
  11. Feldmann M., Beverley P. C., Dunkley M., Kontiainen S. Different Ly antigen phenotypes of in vitro induced helper and suppressor cells. Nature. 1975 Dec 18;258(5536):614–616. doi: 10.1038/258614a0. [DOI] [PubMed] [Google Scholar]
  12. Folch H., Yoshinaga M., Waksman B. H. Regulation of lymphocyte responses in vitro. 3. Inhibition by adherent cells of the T-lymphocyte response to phytohemagglutinin. J Immunol. 1973 Mar;110(3):835–839. [PubMed] [Google Scholar]
  13. Gershon R. K. T cell control of antibody production. Contemp Top Immunobiol. 1974;3:1–40. doi: 10.1007/978-1-4684-3045-5_1. [DOI] [PubMed] [Google Scholar]
  14. Hartmann K., Dutton R. W., McCarthy M. M., Mishell R. I. Cell components in the immune response. II. Cell attachment separation of immune cells. Cell Immunol. 1970 Jul;1(2):182–189. doi: 10.1016/0008-8749(70)90005-5. [DOI] [PubMed] [Google Scholar]
  15. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  16. Kettman J., Dutton R. W. An in vitro primary immune response to 2,4,6-trinitrophenyl substituted erythrocytes: response against carrier and hapten. J Immunol. 1970 Jun;104(6):1558–1561. [PubMed] [Google Scholar]
  17. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  18. Morse H. C., 3rd, Prescott B., Cross S. S., Stashak P. W., Baker P. J. Regulation of the antibody response to type III pneumococcal polysaccharide. V. Ontogeny of factors influencing the magnitude of the plaque-forming cell response. J Immunol. 1976 Feb;116(2):279–287. [PubMed] [Google Scholar]
  19. Mosier D. E., Johnson B. M. Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes. J Exp Med. 1975 Jan 1;141(1):216–226. doi: 10.1084/jem.141.1.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mosier D. E., Mathieson B. J., Campbell P. S. Ly phenotype and mechanism of action of mouse neonatal suppressor T cells. J Exp Med. 1977 Jul 1;146(1):59–73. doi: 10.1084/jem.146.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olding L. B., Oldstone M. B. Lymphocytes from human newborns abrogate mitosis of their mother's lymphocytes. Nature. 1974 May 10;249(453):161–162. doi: 10.1038/249161a0. [DOI] [PubMed] [Google Scholar]
  22. Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. II. Generation of a population of thymus-derived suppressor lymphocytes. J Exp Med. 1973 Mar 1;137(3):649–659. doi: 10.1084/jem.137.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rich S. S., Rich R. R. Regulatory mechanisms in cell-mediated immune responses. I. Regulation of mixed lymphocyte reactions by alloantigen-activated thymus-derived lymphocytes. J Exp Med. 1974 Dec 1;140(6):1588–1603. doi: 10.1084/jem.140.6.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SIMONSEN M. Graft versus host reactions. Their natural history, and applicability as tools of research. Prog Allergy. 1962;6:349–467. [PubMed] [Google Scholar]
  25. Schrader J. W., Nossal G. J. Effector cell blockade. A new mechanism of immune hyporeactivity induced by multivalent antigens. J Exp Med. 1974 Jun 1;139(6):1582–1598. doi: 10.1084/jem.139.6.1582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Thomas D. W., Roberts W. K., Talmage D. W. Regulation of the immune response: production of a soluble suppressor by immune spleen cells in vitro. J Immunol. 1975 May;114(5):1616–1622. [PubMed] [Google Scholar]
  27. Watanabe N., Kojima S., Shen F. W., Ovary Z. Suppression of IgE antibody production in SJL mice. II. Expression of Ly-1 antigen on helper and nonspecific suppressor T cells. J Immunol. 1977 Feb;118(2):485–488. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES