Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Jan;137(1):69–72. doi: 10.1128/jb.137.1.69-72.1979

Phosphate transport in arsenate-resistant mutants of Micrococcus lysodeikticus.

H Alfasi, D Friedberg, I Froedberg
PMCID: PMC218419  PMID: 762027

Abstract

Two types of arsenate-resistant mutants of Micrococcus lysodeikticus were found: (i) mutants that grow in the presence of 10 mM but not 1 mM phosphate (Pi) with low uptake rate for Pi and arsenate, and (ii) mutants able to grow in the presence of 10 mM and 1 mM Pi, with a near-normal uptake rate for Pi but a low one for arsenate. The Km values for Pi transport and the Ki values for its competitive inhibition by arsenate were similar for the mutants and the wild type. Similar to the wild type, the mutants also accumulated Pi to high concentrations. In all strains, the transport of Pi was subject to repression by Pi. Mutant types showed lower Vmax but unaltered Km values for arsenate as compared to the wild type, and they accumulated arsenate to markedly lower levels. The results suggest a two-component transport system common to Pi and arsenate.

Full text

PDF
69

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett R. L., Malamy M. H. Arsenate resistant mutants of Escherichia coli and phosphate transport. Biochem Biophys Res Commun. 1970 Jul 27;40(2):496–503. doi: 10.1016/0006-291x(70)91036-3. [DOI] [PubMed] [Google Scholar]
  2. Friedberg I., Avigad G. Fine structure of polyphosphate granules in Micrococcus lysodeikticus. Isr J Med Sci. 1970 Jul-Aug;6(4):511–518. [PubMed] [Google Scholar]
  3. Friedberg I., Avigad G. Structures containing polyphosphate in Micrococcus lysodeikticus. J Bacteriol. 1968 Aug;96(2):544–553. doi: 10.1128/jb.96.2.544-553.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Friedberg I. Phosphate transport in Micrococcus lysodeikticus. Biochim Biophys Acta. 1977 May 2;466(3):451–460. doi: 10.1016/0005-2736(77)90338-8. [DOI] [PubMed] [Google Scholar]
  5. Friedberg I. The effect of ionophores on phosphate and arsenate transport in Micrococcus lysodeikticus. FEBS Lett. 1977 Sep 15;81(2):264–266. doi: 10.1016/0014-5793(77)80531-0. [DOI] [PubMed] [Google Scholar]
  6. HAROLD F. M., HAROLD R. L., ABRAMS A. A MUTANT OF STREPTOCOCCUS FAECALIS DEFECTIVE IN PHOSPHATE UPTAKE. J Biol Chem. 1965 Jul;240:3145–3153. [PubMed] [Google Scholar]
  7. Harold F. M., Baarda J. R. Interaction of arsenate with phosphate-transport systems in wild- type and mutant Streptococcus faecalis. J Bacteriol. 1966 Jun;91(6):2257–2262. doi: 10.1128/jb.91.6.2257-2262.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harold F. M., Spitz E. Accumulation of arsenate, phosphate, and aspartate by Sreptococcus faecalis. J Bacteriol. 1975 Apr;122(1):266–277. doi: 10.1128/jb.122.1.266-277.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MITCHELL P. Transport of phosphate across the osmotic barrier of Micrococcus pyogenes; specificity and kinetics. J Gen Microbiol. 1954 Aug;11(1):73–82. doi: 10.1099/00221287-11-1-73. [DOI] [PubMed] [Google Scholar]
  10. Medveczky N., Rosenberg H. Phosphate transport in Escherichia coli. Biochim Biophys Acta. 1971 Aug 13;241(2):494–506. doi: 10.1016/0005-2736(71)90048-4. [DOI] [PubMed] [Google Scholar]
  11. Rosenberg H., Gerdes R. G., Chegwidden K. Two systems for the uptake of phosphate in Escherichia coli. J Bacteriol. 1977 Aug;131(2):505–511. doi: 10.1128/jb.131.2.505-511.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberg H., La Nauze J. M. The isolation of a mutant of Bacillus cereus deficient in phosphate uptake. Biochim Biophys Acta. 1968 Mar 11;156(2):381–388. doi: 10.1016/0304-4165(68)90268-7. [DOI] [PubMed] [Google Scholar]
  13. Rosenberg H., Medveczky N., La Nauze J. M. Phosphate transport in Bacillus cereus. Biochim Biophys Acta. 1969 Oct 14;193(1):159–167. doi: 10.1016/0005-2736(69)90069-8. [DOI] [PubMed] [Google Scholar]
  14. Willsky G. R., Bennett R. L., Malamy M. H. Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J Bacteriol. 1973 Feb;113(2):529–539. doi: 10.1128/jb.113.2.529-539.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Willsky G. R., Malamy M. H. The loss of the phoS periplasmic protein leads to a change in the specificity of a constitutive inorganic phosphate transport system in Escherichia coli. Biochem Biophys Res Commun. 1974 Sep 9;60(1):226–233. doi: 10.1016/0006-291x(74)90195-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES