Abstract
Membrane-bound antigens responsible for induction of a secondary allogeneic murine cytolytic T-cell (CTL) response have been obtained in a soluble, biologically active form by deoxycholate solubilization of tumor cell plasma membranes. The active proteins are soluble by the criteria of both ultracentrifugation and gel filtration. The immunological specificity of the induced CTL and removal of the activity from solution by treatment with B6 anti-P815 (anti-H-2d) antiserum and Protein A-Sepharose demonstrate that the CTL-inducing activity is dependent upon solubilized major histocompatibility complex antigens.
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- Burakoff S. J., Germain R. N., Dorf M. E., Benacerrah B. Inhibition of cell-mediated cytolysis of trinitrophenyl-derivatized target cells by alloantisera directed to the products of the K and D loci of the H-2 complex. Proc Natl Acad Sci U S A. 1976 Feb;73(2):625–629. doi: 10.1073/pnas.73.2.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crumpton M. J., Snary D. Preparation and properties of lymphocyte plasma membrane. Contemp Top Mol Immunol. 1974;3:27–56. doi: 10.1007/978-1-4684-2838-4_2. [DOI] [PubMed] [Google Scholar]
- Engers H. D., Thomas K., Cerottini J. C., Brunner K. T. Generation of cytotoxic T lymphocytes in vitro. V. Response of normal and immune spleen cells to subcellular alloantigens. J Immunol. 1975 Aug;115(2):356–360. [PubMed] [Google Scholar]
- Henning R., Milner R. J., Reske K., Cunningham B. A., Edelman G. M. Subunit structure, cell surface orientation, and partial amino-acid sequences of murine histocompatibility antigens. Proc Natl Acad Sci U S A. 1976 Jan;73(1):118–122. doi: 10.1073/pnas.73.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Häyry P., Anderson L. C. Generation of T memory cells in one-way mixed lymphocyte culture. IV. Primary and secondary responses to soluble and insoluble membrane preparations and to ultraviolet-light-inactivated stimulator cells. Scand J Immunol. 1976;5(4):391–399. doi: 10.1111/j.1365-3083.1976.tb00293.x. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Schwartz B. D., Kato K., Cullen S. E., Nathenson S. G. H-2 histocompatibility alloantigens. Some biochemical properties of the molecules solubilized by NP-40 detergent. Biochemistry. 1973 May 22;12(11):2157–2164. doi: 10.1021/bi00735a023. [DOI] [PubMed] [Google Scholar]
- Schwartz B. D., Nathenson S. G. Isolation of H-2 alloantigens solubilized by the detergent NP-40. J Immunol. 1971 Nov;107(5):1363–1367. [PubMed] [Google Scholar]
- Springer T. A., Strominger J. L., Mann D. Partial purification of detergent-soluble HL-A antigen and its cleavage by papain. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1539–1543. doi: 10.1073/pnas.71.4.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strominger J. L., Humphreys R. E., McCune J. M., Parham P., Robb R., Springer T., Terhorst C. The immunoglobulin-like structure of human histocompatibility antigens. Fed Proc. 1976 Apr;35(5):1177–1182. [PubMed] [Google Scholar]
- Wagner H., Hess M., Feldmann M., Röllinghoff M. Secondary cytotoxic allograft responses in vitro. III. The immunogenicity of allogeneic membrane fragments. Transplantation. 1976 Apr;21(4):282–288. doi: 10.1097/00007890-197604000-00002. [DOI] [PubMed] [Google Scholar]