Abstract
Abelson murine leukemia virus (A-MuLV)-transformed fibroblast nonproducer cells were used to prepare A-MuLV stocks containing a number of different helper viruses. The oncogenicity of the A-MuLV stocks was tested by animal inoculation and their ability to transform normal mouse bone marrow cells was measured in vitro. All of the A-MuLV stocks transformed fibroblast cells efficiently. However, only A-MuLV stocks prepared with helper viruses that are highly oncogenic were efficient in vivo and in vitro in hematopoietic cell transformation. In addition, inefficient helpers did not establish a stable infection in lymphoid nonproducer cells. Thus, helper virus has a more central role in lymphoid cell transformation than in fibroblast cell transformation.
Full Text
The Full Text of this article is available as a PDF (1,017.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AXELRAD A. A., STEEVES R. A. ASSAY FOR FRIEND LEUKEMIA VIRUS: RAPID QUANTITATIVE METHOD BASED ON ENUMERATION OF MACROSCOPIC SPLEEN FOCI IN MICE. Virology. 1964 Nov;24:513–518. doi: 10.1016/0042-6822(64)90199-0. [DOI] [PubMed] [Google Scholar]
- Aaronson S. A., Jainchill J. L., Todaro G. J. Murine sarcoma virus transformation of BALB-3T3 cells: lack of dependence on murine leukemia virus. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1236–1243. doi: 10.1073/pnas.66.4.1236. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Aaronson S. A., Rowe S. P. Nonproducer clones of murine sarcoma virus transformed BALB-3T3 cells. Virology. 1970 Sep;42(1):9–19. doi: 10.1016/0042-6822(70)90233-3. [DOI] [PubMed] [Google Scholar]
- Abelson H. T., Rabstein L. S. Lymphosarcoma: virus-induced thymic-independent disease in mice. Cancer Res. 1970 Aug;30(8):2213–2222. [PubMed] [Google Scholar]
- Arnstein P., Riggs J. L., Oshiro L. S., Huebner R. J., Lennette E. H. Induction of lymphoma and associated xenotropic type C virus in C57L mice by whole-body irradiation. J Natl Cancer Inst. 1976 Nov;57(5):1085–1090. doi: 10.1093/jnci/57.5.1085. [DOI] [PubMed] [Google Scholar]
- Baltimore D., Silverstone A. E., Kung P. C., Harrison T. A., McCaffrey R. P. Specialized DNA polymerases in lymphoid cells. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):63–72. doi: 10.1101/sqb.1977.041.01.010. [DOI] [PubMed] [Google Scholar]
- Bassin R. H., Tuttle N., Fischinger P. J. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int J Cancer. 1970 Jul 15;6(1):95–107. doi: 10.1002/ijc.2910060114. [DOI] [PubMed] [Google Scholar]
- Bassin R. H., Tuttle N., Fischinger P. J. Rapid cell culture assay technic for murine leukaemia viruses. Nature. 1971 Feb 19;229(5286):564–566. doi: 10.1038/229564b0. [DOI] [PubMed] [Google Scholar]
- Datta S. K., Melief C. J., Schwartz R. S. Lymphocytes and leukemia viruses: tropism and transtropism of murine leukemia virus. J Natl Cancer Inst. 1975 Aug;55(2):425–432. [PubMed] [Google Scholar]
- Declève A., Lieberman M., Ihle J. N., Kaplan H. S. Biological and serological characterization of radiation leukemia virus. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4675–4679. doi: 10.1073/pnas.73.12.4675. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J Virol. 1969 Feb;3(2):126–132. doi: 10.1128/jvi.3.2.126-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huebner R. J., Hartley J. W., Rowe W. P., Lane W. T., Capps W. I. Rescue of the defective genome of Moloney sarcoma virus from a noninfectious hamster tumor and the production of pseudotype sarcoma viruses with various murine leukemia viruses. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1164–1169. doi: 10.1073/pnas.56.4.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jolicoeur P., Baltimore D. Effect of the Fv-1 locus on the titration of murine leukemia viruses. J Virol. 1975 Dec;16(6):1593–1598. doi: 10.1128/jvi.16.6.1593-1598.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Martin G. S. Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature. 1970 Sep 5;227(5262):1021–1023. doi: 10.1038/2271021a0. [DOI] [PubMed] [Google Scholar]
- Parks W. P., Howk R. S., Anisowicz A., Scolnick E. M. Deletion mapping of moloney type C virus: polypeptide and nucleic acid expression in different transforming virus isolates. J Virol. 1976 May;18(2):491–503. doi: 10.1128/jvi.18.2.491-503.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peebles P. T., Bassin R. H., Haapala D. K., Phillips L. A., Nomura S., Fischinger P. J. Rescue of murine sarcoma virus from a sarcoma-positive leukemia-negative cell line: requirement for replicating leukemia virus. J Virol. 1971 Nov;8(5):690–694. doi: 10.1128/jvi.8.5.690-694.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peebles P. T., Gerwin B. I., Scolnick E. M. Murine sarcoma virus defectiveness: serological detection of only helper virus reverse transcriptase in sarcoma virus rescued from nonmurine S + L-cells. Virology. 1976 Apr;70(2):313–323. doi: 10.1016/0042-6822(76)90274-9. [DOI] [PubMed] [Google Scholar]
- Peeples P. T., Gerwin B. I., Papageorge A. G., Smith S. G. Murine sarcoma virus defectiveness. Viral polymerase expression murine and nonmurine host cells transformed by S+L-type murine sarcoma virus. Virology. 1975 Oct;67(2):344–355. doi: 10.1016/0042-6822(75)90436-5. [DOI] [PubMed] [Google Scholar]
- Rosenberg N., Baltimore D. A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J Exp Med. 1976 Jun 1;143(6):1453–1463. doi: 10.1084/jem.143.6.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberg N., Baltimore D., Scher C. D. In vitro transformation of lymphoid cells by Abelson murine leukemia virus. Proc Natl Acad Sci U S A. 1975 May;72(5):1932–1936. doi: 10.1073/pnas.72.5.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
- Scher C. D. Effect of pseudotype on Abelson virus and Kirsten sarcoma virus-induced leukemia. J Exp Med. 1978 Apr 1;147(4):1044–1053. doi: 10.1084/jem.147.4.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scher C. D., Siegler R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature. 1975 Feb 27;253(5494):729–731. doi: 10.1038/253729a0. [DOI] [PubMed] [Google Scholar]
- Scolnick E. M., Howk R. S., Anisowicz A., Peebles P. T., Scher C. D., Parks W. P. Separation of sarcoma virus-specific and leukemia virus-specific genetic sequences of Moloney sarcoma virus. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4650–4654. doi: 10.1073/pnas.72.11.4650. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vogt P. K. Spontaneous segregation of nontransforming viruses from cloned sarcoma viruses. Virology. 1971 Dec;46(3):939–946. doi: 10.1016/0042-6822(71)90092-4. [DOI] [PubMed] [Google Scholar]
- Wright B. S., O'Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967 Sep;27(9):1672–1677. [PubMed] [Google Scholar]