Abstract
The family of glycoproteins called gp70 includes molecules that are the main constituent of murine C-type viral envelopes, and some that are expressed as mendelian constituents of thymocyte plasma membranes in the absence of virions. To investigate further the relation of viral gp70s to plasma- membrane gp70s we compared peptide maps of gp70s derived by immunoprecipitation from cells infected with chosen viruses and from various thymocytes and leukemiacells known to express one or more of three immunogenetically defined gp70 types: Glx-gp70, X-gp70, and O-gp70. Maps of gp70 from cultured cells infected with ecotropic and xenotropic viruses were distinguishable from one another, and in general resembled gp70 maps prepared directly from ecotropic and xenotropic virions respectively. Maps of gp70s immunoprecipitated from thymocytes of five mouse strains and from two A strain T-cell leukemias also fell into two distinguishable and generally corresponding patterns. Thus peptide-mapping substantiates earlier conclusions that viral gp70s and plasma-membrane gp70s inherited independently of virus-production are highly related or identical molecules. The gp70 maps of thymocytes from B6, B6-G(+IX), 129, and A mice formed a group resembling the map from cultured cells infected with xenotropic virus. Thymocytes from AKR mice, and the two A strain leukemias, gave gp70 maps conforming more to the second pattern, that of cultured cells infected with ecotropic virus. This second pattern probably comprises at least two gp70 types, one of which is X-gp70. Our data indicate that the G(IX)-gp70 and O-gp70 sub-species of gp70 expressed in the cell populations we have studied are coded by xenotropic viral genomes, and X-gp70 by ecotropic viral genomes.
Full Text
The Full Text of this article is available as a PDF (681.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
- Del Vellano B. C., Nave B., Croker B. P., Lerner R. A., Dixon F. J. The oncornavirus glycoprotein gp69/71: a constituent of the surface of normal and malignant thymocytes. J Exp Med. 1975 Jan 1;141(1):172–187. doi: 10.1084/jem.141.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lerner R. A., Wilson C. B., Villano B. C., McConahey P. J., Dixon F. J. Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycorprotein, gp70. J Exp Med. 1976 Jan 1;143(1):151–166. doi: 10.1084/jem.143.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McClintock P. R., Ihle J. N., Joseph D. R. Expression of AKR murine leukemia virus gp71-like and BALB(X) gp-71-like antigens in normal mouse tissues in the absence of overt virus expression. J Exp Med. 1977 Aug 1;146(2):422–434. doi: 10.1084/jem.146.2.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Donnell P. V., Stockert E. Induction of GIX antigen and gross cell surface antigen after infection by ecotropic and xenotropic murine leukemia viruses in vitro. J Virol. 1976 Dec;20(3):545–554. doi: 10.1128/jvi.20.3.545-554.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Obata Y., Ikeda H., Stockert E., Boyse E. A. Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus. J Exp Med. 1975 Jan 1;141(1):188–197. doi: 10.1084/jem.141.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strand M., Lilly F., August J. T. Genetic control of the expression of murine leukemia virus proteins in tissues of normal, young adult mice. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1117–1122. doi: 10.1101/sqb.1974.039.01.127. [DOI] [PubMed] [Google Scholar]
- Tung J. S., Fleissner E., Vitetta E. S., Boyse E. A. Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs. absence of GIX antigen. J Exp Med. 1975 Aug 1;142(2):518–523. doi: 10.1084/jem.142.2.518. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tung J. S., Shen F. W., Fleissner E., Boyse E. A. X-gp70: a third molecular species of the envelope protein gp70 of murine leukemia virus, expressed on mouse lymphoid cells. J Exp Med. 1976 Apr 1;143(4):969–974. doi: 10.1084/jem.143.4.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tung J. S., Vitetta E. S., Fleissner E., Boyse E. A. Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus. J Exp Med. 1975 Jan 1;141(1):198–205. doi: 10.1084/jem.141.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]