Abstract
B lymphocytes capable of generating primary IgM and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do primary IgM PFC. IgG memroy cells arising after one injection of antigen are divided into two groups, one of which expresses surface IgD while the other has no detectable membrane IgD. PFC generated from the IgG memory cells lacking surface IgD show a higher average avidity than those arising from IgD-positive IgG memory cells, indicating that mature IgG memory cells do not have surface IgD. After more than one injection of antigen, few, if any, IgG memory cells have surface IgD. IgG PFC arising in primary or secondary immune response lack membrane- bound IgD. These data provide the outlines for a B-cell maturation pathway in which IgD marks unprimed and early memory B cells and is lost in mature memory cells. Studies presented here were conducted by isolating IgD+ and IgD- cells with the fluorescence-activated cell sorter and functional testing of the isolated populations in adoptive transfer experiments.
Full Text
The Full Text of this article is available as a PDF (881.2 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Andersson B. Studies on the regulation of avidity at the level of the single antibody-forming cell. The effect of antigen dose and time after immunization. J Exp Med. 1970 Jul 1;132(1):77–88. doi: 10.1084/jem.132.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bonner W. A., Hulett H. R., Sweet R. G., Herzenberg L. A. Fluorescence activated cell sorting. Rev Sci Instrum. 1972 Mar;43(3):404–409. doi: 10.1063/1.1685647. [DOI] [PubMed] [Google Scholar]
- Cambier J. C., Vitetta E. S., Uhr J. W., Kettman J. R. B-cell tolerance. II. Trinitrophenyl human gamma globulin-induced tolerance in adult and neonatal murine B cells responsive to thymus-dependent and independent forms of the same hapten. J Exp Med. 1977 Mar 1;145(3):778–783. doi: 10.1084/jem.145.3.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cebra J. J., Goldstein G. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. J Immunol. 1965 Aug;95(2):230–245. [PubMed] [Google Scholar]
- Coffman R. L., Cohn M. The class of surface immunoglobulin on virgin and memory B lymphocytes. J Immunol. 1977 May;118(5):1806–1815. [PubMed] [Google Scholar]
- Cooper M. D., Kearney J. F., Lawton A. R., Abney E. R., Parkhouse R. M., Preud'homme J. L., Seligmann M. Generation of immunoglobulin class diversity in b cells: a discussion with emphasis on idg development. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):573–581. [PubMed] [Google Scholar]
- Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
- Herzenberg L. A., Herzenberg L. A., Black S. J., Loken M. R., Okumura K., van der Loo W., Osborne B. A., Hewgill D., Goding J. W., Gutman G. Surface markers and functional relationships of cells involved in murine B-lymphocyte differentiation. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):33–45. doi: 10.1101/sqb.1977.041.01.007. [DOI] [PubMed] [Google Scholar]
- Hood L., Campbell J. H., Elgin S. C. The organization, expression, and evolution of antibody genes and other multigene families. Annu Rev Genet. 1975;9:305–353. doi: 10.1146/annurev.ge.09.120175.001513. [DOI] [PubMed] [Google Scholar]
- Jones P. P., Cebra J. J., Herzenberg L. A. Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype. J Exp Med. 1974 Mar 1;139(3):581–599. doi: 10.1084/jem.139.3.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- Lawton A. R., 3rd, Cooper M. D. Modification of B lymphocyte differentiation by anti-immunoglobulins. Contemp Top Immunobiol. 1974;3:193–225. doi: 10.1007/978-1-4684-3045-5_8. [DOI] [PubMed] [Google Scholar]
- Mason D. W. The class of surface immunoglobulin on cells carrying IgG memory in rat thoracic duct lymph: the size of the subpopulation mediating IgG memory. J Exp Med. 1976 May 1;143(5):1122–1130. doi: 10.1084/jem.143.5.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okumura K., Julius M. H., Tsu T., Herzenberg L. A., Herzenberg L. A. Demonstration that IgG memory is carried by IgG-bearing cells. Eur J Immunol. 1976 Jul;6(7):467–472. doi: 10.1002/eji.1830060704. [DOI] [PubMed] [Google Scholar]
- Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zan-Bar I., Strober S., Vitetta E. S. The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes. I. Surface immunoglobulin isotypes on primed B cells in the spleen. J Exp Med. 1977 May 1;145(5):1188–1205. doi: 10.1084/jem.145.5.1188. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zan-Bar I., Vitetta E. S., Strober S. The relationship between surface immunoglobulin isotype and immune function of murine B lymphocytes II. Surface immunoglobulin isotopes on unprimed B cells in the spleen. J Exp Med. 1977 May 1;145(5):1206–1215. doi: 10.1084/jem.145.5.1206. [DOI] [PMC free article] [PubMed] [Google Scholar]