Abstract
Marrow stem cell lines from old donors and those from young controls gave equally rapid rates of colony growth on spleens of irradiated mice. Old and young stem cell lines competed equally well with chromosomally marked marrow stem cells from a young donor in producing cell types that are stimulated by bleeding; old cells competed 70% as well as young in producing cell types stimulated by phytohemagglutinin (PHA) in vitro. After a single serial transplantation, the rates of colony growth declined 1.5- to 2.5-fold, and the ability to compete declined 2- to 4-fold for bleeding-stimulated and 4- to 10-fold for PHA- stimulated cells. Thus, immediate stem cell proliferative capacities decline much more after one serial transplantation than after a lifetime of normal function.
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Selected References
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- Albright J. W., Makinodan T. Decline in the growth potential of spleen-colonizing bone marrow stem cells of long-lived aging mice. J Exp Med. 1976 Nov 2;144(5):1204–1213. doi: 10.1084/jem.144.5.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boggs D. R., Marsh J. C., Chervenick P. A., Cartwright G. E., Wintrobe M. M. Factors influencing hematopoietic spleen colony formation in irradiated mice. 3. The effect of repetitive irradiation upon proliferative ability of colony-forming cells. J Exp Med. 1967 Nov 1;126(5):871–880. doi: 10.1084/jem.126.5.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CUDKOWICZ G., UPTON A. C., SHEARER G. M., HUGHES W. L. LYMPHOCYTE CONTENT AND PROLIFERATIVE CAPACITY OF SERIALLY TRANSPLANTED MOUSE BONE MARROW. Nature. 1964 Jan 11;201:165–167. doi: 10.1038/201165a0. [DOI] [PubMed] [Google Scholar]
- Farrar J. J., Loughman B. E., Nordin A. A. Lymphopoietic potential of bone marrow cells from aged mice: comparison of the cellular constituents of bone marrow from young and aged mice. J Immunol. 1974 Mar;112(3):1244–1249. [PubMed] [Google Scholar]
- Harrison D. E., Astle C. M., Doubleday J. W. Cell lines from old immunodeficient donors give normal responses in young recipients. J Immunol. 1977 Apr;118(4):1223–1227. [PubMed] [Google Scholar]
- Harrison D. E. Normal function of transplanted marrow cell lines from aged mice. J Gerontol. 1975 May;30(3):279–285. doi: 10.1093/geronj/30.3.279. [DOI] [PubMed] [Google Scholar]
- Harrison D. E. Normal production of erythrocytes by mouse marrow continuous for 73 months. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3184–3188. doi: 10.1073/pnas.70.11.3184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaliss N. Jerne plaque assay for antibody-forming spleen cells: some technical modifications. Transplantation. 1971 Aug;12(2):146–147. doi: 10.1097/00007890-197108000-00009. [DOI] [PubMed] [Google Scholar]
- Ogden D. A., Mickliem H. S. The fate of serially transplanted bone marrow cell populations from young and old donors. Transplantation. 1976 Sep;22(3):287–293. doi: 10.1097/00007890-197609000-00010. [DOI] [PubMed] [Google Scholar]
- Price G. B., Makinodan T. Immunologic deficiencies in senescence. II. Characterization of extrinsic deficiencies. J Immunol. 1972 Feb;108(2):413–417. [PubMed] [Google Scholar]
- SIMINOVITCH L., TILL J. E., MCCULLOCH E. A. DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE. J Cell Physiol. 1964 Aug;64:23–31. doi: 10.1002/jcp.1030640104. [DOI] [PubMed] [Google Scholar]
- TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]