Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1978 May 1;147(5):1395–1404. doi: 10.1084/jem.147.5.1395

T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells

PMCID: PMC2184279  PMID: 148487

Abstract

We have determined the Ly phenotype of the T lymphocytes which proliferate in response to mutant H-2K and H-2D alloantigens in primary mixed lymphocyte culture. Responder T cells proliferating in reciprocal cultures of H-2d(KdDd) and H-2da(KdDda) lymphocytes were typed Ly 2+ through selective depletion with specific alloantiserum plus complement. Further, B6-Ly 1a lymphocytes proliferating in response to B6-H-2ba and B6-H-2bf stimulators were typed as Ly 1+2+ through similar analysis. These results are discussed with regard to their impact on views of lymphocyte differentiation and factors determining the identity of alloreactive lymphocytes.

Full Text

The Full Text of this article is available as a PDF (645.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alter B. J., Schendel D. J., Bach M. L., Bach F. H., Klein J., Stimpfling J. H. Cell-mediated lympholysis. Importance of serologically defined H-2 regions. J Exp Med. 1973 May 1;137(5):1303–1309. doi: 10.1084/jem.137.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bach F. H., Widmer M. B., Bach M. L., Klein J. Serologically defined and lymphocyte-defined components of the major histocompatibility complex in the mouse. J Exp Med. 1972 Dec 1;136(6):1430–1444. doi: 10.1084/jem.136.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bailey D. W., Kohn H. I. Inherited histocompatibility changes in progeny of irradiated and unirradiated inbred mice. Genet Res. 1965 Nov;6(3):330–340. doi: 10.1017/s0016672300004225. [DOI] [PubMed] [Google Scholar]
  4. Boyse E. A., Itakura K., Stockert E., Iritani C. A., Miura M. Ly-C: a third locus specifying alloantigens expressed only on thymocytes and lymphocytes. Transplantation. 1971 Mar;11(3):351–353. [PubMed] [Google Scholar]
  5. Boyse E. A., Miyazawa M., Aoki T., Old L. J. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc Lond B Biol Sci. 1968 Jun 11;170(1019):175–193. doi: 10.1098/rspb.1968.0032. [DOI] [PubMed] [Google Scholar]
  6. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Click R. E., Benck L., Alter B. J. Immune responses in vitro. I. Culture conditions for antibody synthesis. Cell Immunol. 1972 Feb;3(2):264–276. doi: 10.1016/0008-8749(72)90165-7. [DOI] [PubMed] [Google Scholar]
  9. David C. S., Shreffler D. C., Frelinger J. A. New lymphocyte antigen system (Lna) controlled by the Ir region of the mouse H-2 complex. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2509–2514. doi: 10.1073/pnas.70.9.2509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Flaherty L., Bennett D. Histoincompatibilities found between congenic strains which differ at loci determing differentiation antigens. Transplantation. 1973 Nov;16(5):505–514. doi: 10.1097/00007890-197311000-00016. [DOI] [PubMed] [Google Scholar]
  11. Forman J., Klein J. Immunogeneic analysis of H-2 mutations. II. Cellular immunity to the H-2DA mutation. J Immunol. 1975 Sep;115(3):711–715. [PubMed] [Google Scholar]
  12. Jandinski J., Cantor H., Tadakuma T., Peavy D. L., Pierce C. W. Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1382–1390. doi: 10.1084/jem.143.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  14. McKenzie I. F., Snell G. D. Ly-4.2: a cell membrane alloantigen of murine B lymphocytes. I. Population studies. J Immunol. 1975 Feb;114(2 Pt 2):848–855. [PubMed] [Google Scholar]
  15. Niederhuber J. E., Frelinger J. A., Dugan E., Coutinho A., Shreffler D. C. Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera. J Immunol. 1975 Dec;115(6):1672–1676. [PubMed] [Google Scholar]
  16. Peck A. B., Bach F. H. A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J Immunol Methods. 1973 Oct;3(2):147–163. doi: 10.1016/0022-1759(73)90030-6. [DOI] [PubMed] [Google Scholar]
  17. Rychlíková M., Démant P., Egorov I. K. Mixed lymphocyte reaction caused by an H-2D mutation (congenic strains-B10.D2 new-B10.504). Folia Biol (Praha) 1972;18(5):360–363. [PubMed] [Google Scholar]
  18. Shiku H., Takahashi T., Bean M. A., Old L. J., Oettgen H. F. Ly phenotype of cytotoxic T cells for syngeneic tumor. J Exp Med. 1976 Oct 1;144(4):1116–1120. doi: 10.1084/jem.144.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Snell G. D., Cherry M., McKenzie I. F., Bailey D. W. Ly-4, a new locus determining a lymphocyte cell-surface alloantigen in mice. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1108–1111. doi: 10.1073/pnas.70.4.1108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stutman O., Shen F. W., Boyse E. A. Ly phenotype of T cells cytotoxic for syngeneic mouse mammary tumors: evidence for T cell interactions. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5667–5671. doi: 10.1073/pnas.74.12.5667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wettstein P. J., Frelinger J. A. H-2 effects on cell-cell interactions in the response to single non-H-2 alloantigens. II. H-2D region control of H-7.1-specific stimulator function in mixed lymphocyte culture and susceptibility to lysis by H-7.1-specific cytotoxic cells. J Exp Med. 1977 Nov 1;146(5):1356–1366. doi: 10.1084/jem.146.5.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Widmer M. B., Alter B. J., Bach F. H., Bach M. L. Lymphocyte reactivity to serologically undetected components of the major histocompatibility complex. Nat New Biol. 1973 Apr 25;242(121):239–241. doi: 10.1038/newbio242239a0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES