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. 1978 Jun 1;147(6):1535–1550. doi: 10.1084/jem.147.6.1535

IgG2a-producing variants of an IgG2b-producing mouse myeloma cell line

PMCID: PMC2184309  PMID: 79633

Abstract

12 variant cell lines producing an IgG2a (kappa) immunoglobulin derived via different routes from the IgG2b (kappa) synthesizing MPC 11 were studied. These variants all have the parental MPC 11 idiotype as shown by a radioimmunoassay. A comparison of the variants by charge, peptide maps, and assembly patterns has shown that most of them differ from one another, and some can be grouped. One group consists of three primary variants generated with two mutagenic agents: these three have almost indistinguishable peptide maps. Two other primary variants which arose in a similar fashion differ markedly from each other and from this group. A second group is comprised of the four secondary variants which arose from two short heavy chain producing primary variants. Other secondary variants and the one spontaneously arising variant cannot be grouped. Possible genetic mechanisms such as translocation, expression of previously silent genes and recombination are discussed.

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Selected References

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  1. Adetugbo K., Milstein C., Secher D. S. Molecular analysis of spontaneous somatic mutants. Nature. 1977 Jan 27;265(5592):299–304. doi: 10.1038/265299a0. [DOI] [PubMed] [Google Scholar]
  2. Baumal R., Potter M., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG. J Exp Med. 1971 Nov 1;134(5):1316–1334. doi: 10.1084/jem.134.5.1316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birshtein B. K., Preud'homme J. L., Scharff M. D. Variants of mouse myeloma cells that produce short immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3478–3482. doi: 10.1073/pnas.71.9.3478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birshtein B. K., Turner K. J., Cebra J. J. Structure of heavy chain from strain 13 guinea pig immunoglobulin-G(2). I. Isolation of cyanogen bromide fragments. Biochemistry. 1971 Jan 5;10(1):1–8. doi: 10.1021/bi00777a001. [DOI] [PubMed] [Google Scholar]
  5. Bosma M. J., Bosma G. C. Congenic mouse strains: the expression of a hidden immunoglobulin allotype in a congenic partner strain of BALB-c mice. J Exp Med. 1974 Mar 1;139(3):512–527. doi: 10.1084/jem.139.3.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bosma M. J., Marks R., De Witt C. L. Quantitation of mouse immunoglobulin allotypes by a modified solid-phase radioimmune assay. J Immunol. 1975 Nov;115(5):1381–1386. [PubMed] [Google Scholar]
  7. Brown J. L., Kato K., Silver J., Nathenson S. G. Notable diversity in peptide composition of murine H-2K and H-2D alloantigens. Biochemistry. 1974 Jul 16;13(15):3174–3178. doi: 10.1021/bi00712a027. [DOI] [PubMed] [Google Scholar]
  8. Coffino P., Scharff M. D. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc Natl Acad Sci U S A. 1971 Jan;68(1):219–223. doi: 10.1073/pnas.68.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  10. Gally J. A., Edelman G. M. Somatic translocation of antibody genes. Nature. 1970 Jul 25;227(5256):341–348. doi: 10.1038/227341a0. [DOI] [PubMed] [Google Scholar]
  11. Guyer R. L., Koshland M. E., Knopf P. M. Immunoglobulin binding by mouse intestinal epithelial cell receptors. J Immunol. 1976 Aug;117(2):587–593. [PubMed] [Google Scholar]
  12. Hausman S. J., Bosma M. J. Alteration of immunoglobulin phenotype in cell culture-adapted lines of two mouse plasmacytomas. J Exp Med. 1975 Oct 1;142(4):998–1010. doi: 10.1084/jem.142.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hozumi N., Tonegawa S. Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3628–3632. doi: 10.1073/pnas.73.10.3628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koskimies S., Birshtein B. K. Primary and secondary variants in immunoglobulin heavy chain production. Nature. 1976 Dec 2;264(5585):480–482. doi: 10.1038/264480a0. [DOI] [PubMed] [Google Scholar]
  15. Kunkel H. G., Natvig J. B., Joslin F. G. A "Lepore" type of hybrid gamma-globulin. Proc Natl Acad Sci U S A. 1969 Jan;62(1):144–149. doi: 10.1073/pnas.62.1.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Laskov R., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J Exp Med. 1970 Mar 1;131(3):515–541. doi: 10.1084/jem.131.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
  18. Natvig J. B., Kunkel H. G. A HYBRID IgG4-IgG2 immunoglobulin. J Immunol. 1974 Apr;112(4):1277–1284. [PubMed] [Google Scholar]
  19. Potter M., Pumphrey J. G., Walters J. L. Growth of primary plasmacytomas in the mineral oil-conditioned peritoneal environment. J Natl Cancer Inst. 1972 Jul;49(1):305–308. [PubMed] [Google Scholar]
  20. Preud'Homme J. L., Birshtein B. K., Scharff M. D. Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1427–1430. doi: 10.1073/pnas.72.4.1427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robertson M. Immunoglobulin genes and the immune response. Nature. 1977 Oct 20;269(5630):648–650. doi: 10.1038/269648a0. [DOI] [PubMed] [Google Scholar]
  22. Scharff M. D. The synthesis, assembly, and secretion of immunoglobulin: a biochemical and genetic approach. Harvey Lect. 1973;(69):125–142. [PubMed] [Google Scholar]
  23. Spring S. B., Nisonoff A. Allotypic markers on Fab fragments of mouse immunoglobulins. J Immunol. 1974 Aug;113(2):470–478. [PubMed] [Google Scholar]
  24. Warner N. L., Herzenberg L. A., Goldstein G. Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins. J Exp Med. 1966 Apr 1;123(4):707–721. doi: 10.1084/jem.123.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]

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