Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1978 Feb 1;147(2):369–384. doi: 10.1084/jem.147.2.369

A mononuclear cell component in experimental immunological glomerulonephritis

PMCID: PMC2184493  PMID: 146726

Abstract

An accelerated form of nephrotoxic serum nephritis in the rat was examined. The experimental model consisted of preimmunization of the rat with rabbit IgG 5 days before injection of subnephrotoxic doses of rabbit anti-rat kidney serum. The immunized rats developed proteinuria during the first 24 h, increasing by 48-96 h. The early 24-h proteinuria correlated with a neutrophilic infiltration of glomeruli and with deposition of rat Ig and C. The 48- to 96-h proteinuria was associated with a glomerular infiltration by mononuclear cells and proliferation of intrinsic glomerular cells. Many of the mononuclear cells were morphologically identical to monocytes and macrophages. [3H]thymidine labeling experiments indicated that the mononuclear cells originated from dividing precursors localized outside the kidney. Preimmunized rats given systemic irradiation (the kidney being protected by a shield) showed loss of the mononuclear cell infiltrate and absence of 48- to 96-h proteinuria. We conclude that mononuclear phagocytes can infiltrate the kidney in immunological glomerular disease and might contribute to the functional abnormalities.

Full Text

The Full Text of this article is available as a PDF (4.0 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COCHRANE C. G., UNANUE E. R., DIXON F. J. A ROLE OF POLYMORPHONUCLEAR LEUKOCYTES AND COMPLEMENT IN NEPHROTOXIC NEPHRITIS. J Exp Med. 1965 Jul 1;122:99–116. doi: 10.1084/jem.122.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cochrane C. G. Immunologic tissue injury mediated by neutrophilic leukocytes. Adv Immunol. 1968;9:97–162. doi: 10.1016/s0065-2776(08)60442-3. [DOI] [PubMed] [Google Scholar]
  3. Dixon F. J. The pathogenesis of glomerulonephritis. Am J Med. 1968 Apr;44(4):493–498. doi: 10.1016/0002-9343(68)90050-8. [DOI] [PubMed] [Google Scholar]
  4. HAMMER D. K., DIXON F. J. Experimental glomerulonephritis. II. Immunologic events in the pathogenesis of nephrotoxic serum nephritis in the rat. J Exp Med. 1963 Jun 1;117:1019–1034. doi: 10.1084/jem.117.6.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HASSON M. W., BEVANS M., SEEGAL B. C. Immediate or delayed nephritis in rats produced by duck anti-rat-kidney sera. AMA Arch Pathol. 1957 Aug;64(2):192–204. [PubMed] [Google Scholar]
  6. Hawkins D., Cochrane C. G. Glomerular basement membrane damage in immunological glomerulonephritis. Immunology. 1968 May;14(5):665–681. [PMC free article] [PubMed] [Google Scholar]
  7. Johnston W. H., Latta H. Acute focal glomerulonephritis in the rabbit induced by injection of Saccharomyces cerevisiae. An electron microscopic study. Lab Invest. 1972 Jun;26(6):741–754. [PubMed] [Google Scholar]
  8. Kondo Y., Shigematsu H., Kobayashi Y. Cellular aspects of rabbit Masugi nephritis. II. Progressive glomerular injuries with crescent formation. Lab Invest. 1972 Dec;27(6):620–631. [PubMed] [Google Scholar]
  9. Leibovich S. J., Ross R. A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro. Am J Pathol. 1976 Sep;84(3):501–514. [PMC free article] [PubMed] [Google Scholar]
  10. Polverini P. J., Cotran P. S., Gimbrone M. A., Jr, Unanue E. R. Activated macrophages induce vascular proliferation. Nature. 1977 Oct 27;269(5631):804–806. doi: 10.1038/269804a0. [DOI] [PubMed] [Google Scholar]
  11. Shigematsu H. Glomerular events during the initial phase of rat Masugi nephritis. Virchows Arch B Cell Pathol. 1970;5(3):187–200. [PubMed] [Google Scholar]
  12. UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. V. STUDIES ON THE INTERACTION OF NEPHROTOXIC ANTIBODIES WITH TISSUE OF THE RAT. J Exp Med. 1965 May 1;121:697–714. doi: 10.1084/jem.121.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. VI. THE AUTOLOGOUS PHASE OF NEPHROTOXIC SERUM NEPHRITIS. J Exp Med. 1965 May 1;121:715–725. doi: 10.1084/jem.121.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. UNANUE E., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. IV. PARTICIPATION OF COMPLEMENT IN NEPHROTOXIC NEPHRITIS. J Exp Med. 1964 Jan 1;119:965–982. doi: 10.1084/jem.119.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Unanue E. R., Dixon F. J. Experimental glomerulonephritis: immunological events and pathogenetic mechanisms. Adv Immunol. 1967;6:1–90. doi: 10.1016/s0065-2776(08)60521-0. [DOI] [PubMed] [Google Scholar]
  16. Unanue E. R. Secretory function of mononuclear phagocytes: a review. Am J Pathol. 1976 May;83(2):396–418. [PMC free article] [PubMed] [Google Scholar]
  17. VASSALLI P., MCCLUSKEY R. T. THE PATHOGENIC ROLE OF THE COAGULATION PROCESS IN RABBIT MASUGI NEPHRITIS. Am J Pathol. 1964 Oct;45:653–677. [PMC free article] [PubMed] [Google Scholar]
  18. VOLKMAN A., GOWANS J. L. THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT. Br J Exp Pathol. 1965 Feb;46:62–70. [PMC free article] [PubMed] [Google Scholar]
  19. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES