Abstract
The biosynthesis of the diglyceride moiety of murein lipoprotein was studied by fusion of labeled phospholipid vesicles with intact cells of Salmonella typhimurium. Phosphatidylglycerol was found to be an excellent donor for the glyceryl moiety in lipoprotein, whereas phosphatidylethanolamine and cardiolipin were not. The incorporation of radioactivity from monoacyl-phosphatidylglycerol into lipoprotein can be attributed to its conversion to phosphatidylglycerol. The results strongly support our hypothesis that the glyceryl residue covalently linked to murein lipoprotein is derived from the nonacylated glycerol moiety of phosphatidylglycerol.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bell R. M., Mavis R. D., Osborn M. J., Vagelos P. R. Enzymes of phospholipid metabolism: localization in the cytoplasmic and outer membrane of the cell envelope of Escherichia coli and Salmonella typhimurium. Biochim Biophys Acta. 1971 Dec 3;249(2):628–635. doi: 10.1016/0005-2736(71)90144-1. [DOI] [PubMed] [Google Scholar]
- Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
- Chattopadhyay P. K., Wu H. C. Biosynthesis of the covalently linked diglyceride in murein lipoprotein of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5318–5322. doi: 10.1073/pnas.74.12.5318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cho K. S., Hong S. D., Cho J. M., Chang C. S., Lee K. S. Studies on the biosynthesis of acylphosphatidylglycerol in Escherichia coli B and B/r. Biochim Biophys Acta. 1976 Jan 18;486(1):47–54. doi: 10.1016/0005-2760(77)90068-6. [DOI] [PubMed] [Google Scholar]
- Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
- Hantke K., Braun V. Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane. Eur J Biochem. 1973 Apr;34(2):284–296. doi: 10.1111/j.1432-1033.1973.tb02757.x. [DOI] [PubMed] [Google Scholar]
- Hirashima A., Wu H. C., Venkateswaran P. S., Inouye M. Two forms of a structural lipoprotein in the envelope of Escherichia coli. Further characterization of the free form. J Biol Chem. 1973 Aug 25;248(16):5654–5659. [PubMed] [Google Scholar]
- Hirschberg C. B., Kennedy E. P. Mechanism of the enzymatic synthesis of cardiolipin in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Mar;69(3):648–651. doi: 10.1073/pnas.69.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Inouye S., Lee N., Inouye M., Wu H. C., Suzuki H., Nishimura Y., Iketani H., Hirota Y. Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane. J Bacteriol. 1977 Oct;132(1):308–313. doi: 10.1128/jb.132.1.308-313.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones N. C., Osborn M. J. Translocation of phospholipids between the outer and inner membranes of Salmonella typhimurium. J Biol Chem. 1977 Oct 25;252(20):7405–7412. [PubMed] [Google Scholar]
- Kennedy E. P., Rumley M. K., Schulman H., Van Golde L. M. Identification of sn-glycero-1-phosphate and phosphoethanolamine residues linked to the membrane-derived Oligosaccharides of Escherichia coli. J Biol Chem. 1976 Jul 25;251(14):4208–4213. [PubMed] [Google Scholar]
- Lin J. J., Wu H. C. Biosynthesis and assembly of envelope lipoprotein in a glycerol-requiring mutant of Salmonella typhimurium. J Bacteriol. 1976 Mar;125(3):892–904. doi: 10.1128/jb.125.3.892-904.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olsen R. W., Ballou C. E. Acyl phosphatidylglycerol. A new phospholipid from Salmonella typhimurium. J Biol Chem. 1971 May 25;246(10):3305–3313. [PubMed] [Google Scholar]
- Rotering H., Braun V. Lipid deficiency in a lipoprotein mutant of Escherichia coli. FEBS Lett. 1977 Nov 1;83(1):41–44. doi: 10.1016/0014-5793(77)80637-6. [DOI] [PubMed] [Google Scholar]
- White D. A., Albright F. R., Lennarz W. J., Schnaitman C. A. Distribution of phospholipid-synthesizing enzymes in the wall and membrane subfractions of the envelope of Escherichia coli. Biochim Biophys Acta. 1971 Dec 3;249(2):636–642. doi: 10.1016/0005-2736(71)90145-3. [DOI] [PubMed] [Google Scholar]
- White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]