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. 1979 Jan;137(1):321–326. doi: 10.1128/jb.137.1.321-326.1979

Control of heterocyst and nitrogenase synthesis in cyanobacteria.

G Stacey, P J Bottomley, C Van Baalen, F R Tabita
PMCID: PMC218453  PMID: 104963

Abstract

The development of the heterocyst by filamentous nitrogen-fixing cyanobacteria provides an attractive model system for studying cellular differentiation. Heterocyst synthesis is repressed by the presence of exogenous combined nitrogen. In this report, it is shown that the tryptophan analog, D,L-7-azatryptophan (Aza-T), is capable of relieving the repressive effect of exogenous NH4NO3 on heterocyst and nitrogenase synthesis. In nitrogen-fixing cultures, the presence of 20 micron Aza-T increases the heterocyst frequency twofold. The glutamate analog, L-methionine-D,L-sulfoximine (MSX), has also been shown to cause a derepression in the synthesis of heterocysts and nitrogenase. However, unlike MSX, Aza-T does not appear to exert its effects by inhibiting the activity of glutamine synthetase. Therefore, glutamine synthetase may not be the sole key to the derepression of heterocyst and nitrogenase development in the cyanobacteria. It is hoped that a study of Aza-T action may lead to the elucidation of a novel control mechanism.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANEY A. L., MARBACH E. P. Modified reagents for determination of urea and ammonia. Clin Chem. 1962 Apr;8:130–132. [PubMed] [Google Scholar]
  2. Dharmawardene M. W., Haystead A., Stewart W. D. Glutamine synthetase of the nitrogen-fixing alga Anabaena cylindrica. Arch Mikrobiol. 1973 Apr 26;90(4):281–295. doi: 10.1007/BF00408924. [DOI] [PubMed] [Google Scholar]
  3. Fay P., Stewart W. D., Walsby A. E., Fogg G. E. Is the heterocyst the site of nitrogen fixation in blue-green algae? Nature. 1968 Nov 23;220(5169):810–812. doi: 10.1038/220810b0. [DOI] [PubMed] [Google Scholar]
  4. Freese E. Sporulation of bacilli, a model of cellular differentiation. Curr Top Dev Biol. 1972;7:85–124. doi: 10.1016/s0070-2153(08)60070-8. [DOI] [PubMed] [Google Scholar]
  5. Gordon J. K., Brill W. J. Derepression of nitrogenase synthesis in the presence of excess NH4+. Biochem Biophys Res Commun. 1974 Aug 5;59(3):967–971. doi: 10.1016/s0006-291x(74)80074-4. [DOI] [PubMed] [Google Scholar]
  6. Kulasooriya S. A., Lang N. J., Fay P. The heterocysts of blue-green algae. 3. Differentiation and nitrogenase activity. Proc R Soc Lond B Biol Sci. 1972 Jun 6;181(1063):199–209. doi: 10.1098/rspb.1972.0046. [DOI] [PubMed] [Google Scholar]
  7. Magasanik B., Prival M. J., Brenchley J. E., Tyler B. M., DeLeo A. B., Streicher S. L., Bender R. A., Paris C. G. Glutamine synthetase as a regulator of enzyme synthesis. Curr Top Cell Regul. 1974;8(0):119–138. doi: 10.1016/b978-0-12-152808-9.50010-9. [DOI] [PubMed] [Google Scholar]
  8. Mitchison G. J., Wilcox M. Alteration in heterocyst pattern of Anabaena produced by 7-azatryptophan. Nat New Biol. 1973 Dec 19;246(155):229–233. doi: 10.1038/newbio246229a0. [DOI] [PubMed] [Google Scholar]
  9. Neilson A., Rippka R., Kunisawa R. Heterocyst formation and nitrogenase synthesis in Anabaena sp. A kinetic study. Arch Mikrobiol. 1971;76(2):139–150. doi: 10.1007/BF00411788. [DOI] [PubMed] [Google Scholar]
  10. Ronzio R. A., Rowe W. B., Meister A. Studies on the mechanism of inhibition of glutamine synthetase by methionine sulfoximine. Biochemistry. 1969 Mar;8(3):1066–1075. doi: 10.1021/bi00831a038. [DOI] [PubMed] [Google Scholar]
  11. Stacey G., Tabita F. R., Van Baalen C. Nitrogen and ammonia assimilation in the cyanobacteria: purification of glutamine synthetase from Anabaena sp. strain CA. J Bacteriol. 1977 Nov;132(2):596–603. doi: 10.1128/jb.132.2.596-603.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stewart W. D., Fitzgerald G. P., Burris R. H. In situ studies on N2 fixation using the acetylene reduction technique. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2071–2078. doi: 10.1073/pnas.58.5.2071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stewart W. D., Haystead A., Pearson H. W. Nitrogenase activity in heterocysts of blue-green algae. Nature. 1969 Oct 18;224(5216):226–228. doi: 10.1038/224226a0. [DOI] [PubMed] [Google Scholar]
  14. Stewart W. D., Rowell P. Effects of L-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindrica. Biochem Biophys Res Commun. 1975 Aug 4;65(3):846–856. doi: 10.1016/s0006-291x(75)80463-3. [DOI] [PubMed] [Google Scholar]
  15. Stewart W. D., Rowell P., Tel-or E. Nitrogen fixation and the heterocyst in blue-green algae. Biochem Soc Trans. 1975;3(3):357–361. doi: 10.1042/bst0030357. [DOI] [PubMed] [Google Scholar]
  16. Thomas J., Meeks J. C., Wolk C. P., Shaffer P. W., Austin S. M. Formation of glutamine from [13n]ammonia, [13n]dinitrogen, and [14C]glutamate by heterocysts isolated from Anabaena cylindrica. J Bacteriol. 1977 Mar;129(3):1545–1555. doi: 10.1128/jb.129.3.1545-1555.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thomas J., Wolk C. P., Shaffer P. W., Austin S. M., Galonsky A. The initial organic products of fixation of 13N-labeled nitrogen gas by the blue-green alga Anabaena cylindrica. Biochem Biophys Res Commun. 1975 Nov 17;67(2):501–507. doi: 10.1016/0006-291x(75)90840-2. [DOI] [PubMed] [Google Scholar]
  18. Weare N. M., Shanmugam K. T. Photoproduction of ammonium ion from N2 in Rhodospirillum rubrum. Arch Microbiol. 1976 Nov 2;110(23):207–213. doi: 10.1007/BF00690229. [DOI] [PubMed] [Google Scholar]
  19. Winkenbach F., Wolk C. P. Activities of enzymes of the oxidative and the reductive pentose phosphate pathways in heterocysts of a blue-green alga. Plant Physiol. 1973 Nov;52(5):480–483. doi: 10.1104/pp.52.5.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wolk C. P. Movement of carbon from vegetative cells to heterocysts in Anabaena cylindrica. J Bacteriol. 1968 Dec;96(6):2138–2143. doi: 10.1128/jb.96.6.2138-2143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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