Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1979 Jan 1;149(1):158–171. doi: 10.1084/jem.149.1.158

Human macromolecular insoluble cold globulin (MICG). I. T-cell origin of T-MICG and null cell origin of N-MICG

PMCID: PMC2184745  PMID: 84041

Abstract

Although surface immunoglobulin characterizes B cells in man, there are few surface markers that distinguish T cells. We have described a new protein synthesized in human T cells, termed T-MICG. This protein is a macromolecule of 225,000 daltons, is insoluble in the cold, and migrates as a beta-globulin on electrophoresis. Separation of human peripheral blood lymphocytes into T and B-cell populations by rosette sedimentation and anti-human-Fab columns clearly demonstrated the T- cell origin of the 225,000 dalton component. Furthermore, null cells were shown to synthesize a protein of 185,000 daltons, termed N-MICG, with physical properties similar to T-MICG, T-MICG and N-MICG were shown to be antigenically dissimilar, employing antiserum to each of these proteins. The present studies demonstrate two novel cell surface markers, T-MICG and N-MICG, which characterize T cells and null cells, respectively.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderson E., Birchall J. P., Owen J. J. A simple method of lymphocyte purification from human peripheral blood. J Immunol Methods. 1976;11(3-4):297–301. doi: 10.1016/0022-1759(76)90123-x. [DOI] [PubMed] [Google Scholar]
  2. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Billing R., Rafizadeh B., Drew I., Hartman G., Gale R., Terasaki P. Human B-lymphocyte antigens expressed by lymphocytic and myelocytic leukemia cells. I. Detection by rabbit antisera. J Exp Med. 1976 Jul 1;144(1):167–178. doi: 10.1084/jem.144.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Lymphocytes as models for the study of mammalian cellular differentiation. Immunol Rev. 1977 Jan;33:105–124. doi: 10.1111/j.1600-065x.1977.tb00364.x. [DOI] [PubMed] [Google Scholar]
  6. Chess L., Evans R., Humphreys R. E., Strominger J. L., Schlossman S. F. Inhibition of antibody-dependent cellular cytotoxicity and immunoglobulin synthesis by an antiserum prepared against a human B-cell Ia-like molecule. J Exp Med. 1976 Jul 1;144(1):113–122. doi: 10.1084/jem.144.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chess L., Levine H., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset. J Immunol. 1975 Dec;115(6):1483–1487. [PubMed] [Google Scholar]
  8. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens. J Immunol. 1974 Oct;113(4):1113–1121. [PubMed] [Google Scholar]
  9. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goding J. W. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215–226. doi: 10.1016/0022-1759(76)90068-5. [DOI] [PubMed] [Google Scholar]
  11. Greaves M. F., Brown G. Purification of human T and B lymphocytes. J Immunol. 1974 Jan;112(1):420–423. [PubMed] [Google Scholar]
  12. Hauptman S. P. Macromolecular insoluble cold globulin (MICG): a novel protein from mouse lymphocytes--I. Isolation and characterization. Immunochemistry. 1978 Jul;15(7):415–422. doi: 10.1016/0161-5890(78)90068-8. [DOI] [PubMed] [Google Scholar]
  13. Hauptman S. P., Sobczak G. A new protein: macromolecular insoluble could globulin (MICG) derived from mouse thymocytes. Immunol Commun. 1978;7(1):69–79. doi: 10.3109/08820137809033924. [DOI] [PubMed] [Google Scholar]
  14. Hauptman S., Tomasi T. B., Jr A monoclonal IgM protein with antibody-like activity for human albumin. J Clin Invest. 1974 Mar;53(3):932–940. doi: 10.1172/JCI107634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hauptman S., Tomasi T. B., Jr Antibodies to human albumin in cirrhotic sera. J Clin Invest. 1974 Jul;54(1):122–127. doi: 10.1172/JCI107733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  17. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  18. Klareskog L., Sandgerg-Trägårdh L., Rask L., Lindblom J. B., Curman B., Peterson P. A. Chemical properties of human Ia antigens. Nature. 1977 Jan 20;265(5591):248–251. doi: 10.1038/265248a0. [DOI] [PubMed] [Google Scholar]
  19. Laskov R., Lanzerotti R., Scharff M. D. Synthesis, assembly and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells. J Mol Biol. 1971 Mar 14;56(2):327–339. doi: 10.1016/0022-2836(71)90468-2. [DOI] [PubMed] [Google Scholar]
  20. Laskov R., Scharff M. D. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J Exp Med. 1970 Mar 1;131(3):515–541. doi: 10.1084/jem.131.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Marchalonis J. J., Cone R. E., Boehmer H. V. Surface immunoglobulins of peripheral thymus-derived lymphocytes. Immunochemistry. 1974 May;11(5):271–277. doi: 10.1016/0019-2791(74)90206-7. [DOI] [PubMed] [Google Scholar]
  22. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  23. Mittal K. K., Mickey M. R., Singal D. P., Terasaki P. I. Serotyping for homotransplantation. 18. Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation. 1968 Nov;6(8):913–927. doi: 10.1097/00007890-196811000-00006. [DOI] [PubMed] [Google Scholar]
  24. Pernis B., Forni L., Amante L. Immunoglobulin spots on the surface of rabbit lymphocytes. J Exp Med. 1970 Nov;132(5):1001–1018. doi: 10.1084/jem.132.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Preud'homme J. L., Seligmann M. Anti-human immunoglobulin G activity of membrane-bound monoclonal immunoglobulin M in lymphoproliferative disorders. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2132–2135. doi: 10.1073/pnas.69.8.2132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  28. Schlossman S. F., Chess L., Humphreys R. E., Strominger J. L. Distribution of Ia-like molecules on the surface of normal and leukemic human cells. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288–1292. doi: 10.1073/pnas.73.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. TERASAKI P. I., MCCLELLAND J. D. MICRODROPLET ASSAY OF HUMAN SERUM CYTOTOXINS. Nature. 1964 Dec 5;204:998–1000. doi: 10.1038/204998b0. [DOI] [PubMed] [Google Scholar]
  30. Touraine J. L., Hadden J. W., Good R. A. Sequential stages of human T lymphocyte differentiation. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3414–3418. doi: 10.1073/pnas.74.8.3414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Vitetta E. S., Uhr J. W., Boyse E. A. Immunoglobulin synthesis and secretion by cells in the mouse thymus that do not bear theta antigen. Proc Natl Acad Sci U S A. 1973 Mar;70(3):834–838. doi: 10.1073/pnas.70.3.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  34. Winchester R. J., Wang C. Y., Halper J., Hoffman T. Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties. Scand J Immunol. 1976;5(6-7):745–757. doi: 10.1111/j.1365-3083.1976.tb03024.x. [DOI] [PubMed] [Google Scholar]
  35. Wybran J., Fudenberg H. H. Thymus-derived rosette-forming cells. N Engl J Med. 1973 May 17;288(20):1072–1073. doi: 10.1056/NEJM197305172882011. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES