Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Jan;137(1):537–544. doi: 10.1128/jb.137.1.537-544.1979

Ultrastructure and cytochemical localization of laccase in two strains of Leptosphaerulina briosiana (Pollaci) Graham and Luttrell.

L T Simon, D S Bishop, G R Hooper
PMCID: PMC218481  PMID: 104971

Abstract

Substrate specificity tests were used to identify the presence of laccase in two strains of Leptosphaerulina briosiana (Poll.) Graham and Luttrell, an ascomycete which causes leaf spot in alfalfa. Cytochemical localization of monophenol monooxygenase (laccase) as well as the ultrastructures of the two strains were investigated. Laccase was observed in the outer layers of the cell walls of both strains. The ultrastructures of vegetative hyphae of both strains were typical of those found in most ascomycetes.

Full text

PDF
537

Images in this article

539Image
on p.539
541Image
on p.541
542Image
on p.542

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner D. M., Carroll G. C. Fine-structural correlates of growth in hyphae of Ascodesmis sphaerospora. J Bacteriol. 1968 Feb;95(2):658–671. doi: 10.1128/jb.95.2.658-671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Djaczenko W., Cassone A. Visulization of new ultrastructural components in the cell wall of Candida albicans with fixatives containing TAPO. J Cell Biol. 1972 Jan;52(1):186–190. doi: 10.1083/jcb.52.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eppig J. J., Jr, Dumont J. N. Cytochemical localization of tyrosinase activity in pigmented epithelial cells of Rana pipiens and Xenopus laevis larvae. J Ultrastruct Res. 1972 May;39(3):397–410. doi: 10.1016/s0022-5320(72)90031-7. [DOI] [PubMed] [Google Scholar]
  4. Esau K., Cronshaw J. Relation of tobacco mosaic virus to the host cells. J Cell Biol. 1967 Jun;33(3):665–678. doi: 10.1083/jcb.33.3.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Esau K., Cronshaw J. Tubular components in cells of healthy and tobacco mosaic virus-infected Nicotiana. Virology. 1967 Sep;33(1):26–35. doi: 10.1016/0042-6822(67)90090-6. [DOI] [PubMed] [Google Scholar]
  6. FLING M., HOROWITZ N. H., HEINEMANN S. F. The isolation and properties of crystalline tyrosinase from Neurospora. J Biol Chem. 1963 Jun;238:2045–2053. [PubMed] [Google Scholar]
  7. Froehner S. C., Eriksson K. E. Purification and properties of Neurospora crassa laccase. J Bacteriol. 1974 Oct;120(1):458–465. doi: 10.1128/jb.120.1.458-465.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOROWITZ N. H., SHEN S. C. Neurospora tyrosinase. J Biol Chem. 1952 May;197(2):513–520. [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lones G. W., Popkin T. J. Effect of saramycetin on the fine structure of Histoplasma capsulatum. Mycopathol Mycol Appl. 1972 Jan 26;46(1):103–109. doi: 10.1007/BF02051902. [DOI] [PubMed] [Google Scholar]
  11. Lowry R. J., Sussman A. S. Intra-hyphal hyphae in "clock" mutants of Neurospora. Mycologia. 1966 Jul-Aug;58(4):541–548. [PubMed] [Google Scholar]
  12. Mahadevan P. R., Tatum E. L. Localization of structural polymers in the cell wall of Neurospora crassa. J Cell Biol. 1967 Nov;35(2):295–302. doi: 10.1083/jcb.35.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Manocha M. S., Colvin J. R. Structure and composition of the cell wall of Neurospora crassa. J Bacteriol. 1967 Jul;94(1):202–212. doi: 10.1128/jb.94.1.202-212.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NEUFELD H. A., LATTERELL F. M., GREEN L. F., WEINTRAUB R. L. Oxidation of meta-polyhydroxyphenols by enzymes from Piricularia oryzae and Polyporus versicolor. Arch Biochem Biophys. 1958 Aug;76(2):317–327. doi: 10.1016/0003-9861(58)90157-7. [DOI] [PubMed] [Google Scholar]
  15. Novikoff A. B., Albala A., Biempica L. Ultrastructural and cytochemical observations on B-16 and Harding-Passey mouse melanomas. The origin of premelanosomes and compound melanosomes. J Histochem Cytochem. 1968 May;16(5):299–319. doi: 10.1177/16.5.299. [DOI] [PubMed] [Google Scholar]
  16. Okun M. R., Edelstein L. M., Or N., Hamada G., Donnellan B., Lever W. F. Histochemical differentiation of peroxidase-mediated from tyrosinase-mediated melanin formation in mammalian tissues. The biologic significance of peroxidase-mediated oxidation of tyrosine to malanin. Histochemie. 1970;23(4):295–309. doi: 10.1007/BF00278360. [DOI] [PubMed] [Google Scholar]
  17. Okun M. R., Edelstein L., Niebauer G., Hamada G. The histochemical tyrosine-dopa reaction for tyrosinase and its use in localizing tyrosinase activity in mast cells. J Invest Dermatol. 1969 Jul;53(1):39–45. doi: 10.1038/jid.1969.105. [DOI] [PubMed] [Google Scholar]
  18. Parish R. W. The intracellular location of phenol oxidases, peroxidase and phosphatases in the leaves of spinach beet (beta vulgaris L. subspecies vulgaris). Eur J Biochem. 1972 Dec 18;31(3):446–455. doi: 10.1111/j.1432-1033.1972.tb02551.x. [DOI] [PubMed] [Google Scholar]
  19. Roos U. P., Khandjian E. W., Turian G. RNA virus in Allomyces arbuscula: ultrastructural localization during the life-cycle. J Gen Microbiol. 1976 Jul;95(1):87–95. doi: 10.1099/00221287-95-1-87. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES