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. 1979 Mar 1;149(3):713–723. doi: 10.1084/jem.149.3.713

Endotoxin protein is a mitogen and polyclonal activator of human B lymphocytes

PMCID: PMC2184825  PMID: 311814

Abstract

Endotoxin protein (EP) has been shown to be a mitogen and polyclonal activator of human peripheral blood lymphocytes. EP stimulates proliferation of B lymphoyctes in the absence of T cells, and this activation is nonspecific by a number of parameters. Additionally, EP mitogenesis, but not polyclonal activation, is inhibited in the presence of human serum, suggesting that these events are dissociable. In these studies, EP appears to be equivalent to or better than pokeweed mitogen in stimulating nonspecific antibody production in vitro.

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Selected References

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  1. Bona C., Damais C., Dimitriu A., Chedid L., Ciorbaru R., Adam A., Petit J. F., Lederer E., Rosselet J. P. Mitogenic effect of a water-soluble extract of Nocardia opaca: a comparative study with some bacterial adjuvants on spleen and peripheral lymphocytes of four mammalian species. J Immunol. 1974 Jun;112(6):2028–2035. [PubMed] [Google Scholar]
  2. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  3. Brochier J., Bona C., Ciorbaru R., Revillard J. P., Chedid L. A human T-independent B lymphocyte mitogen extracted from Nocardia opaca. J Immunol. 1976 Nov;117(5 Pt 1):1434–1439. [PubMed] [Google Scholar]
  4. Brochier J., Samarut C., Gueho J. P., Revillard J. P. T-dependence of human B lymphocyte proliferative response to mitogens. Int Arch Allergy Appl Immunol. 1976;51(1):101–116. doi: 10.1159/000231582. [DOI] [PubMed] [Google Scholar]
  5. Brown J. M., Leon M. A., Lightbody J. J. Isolation of a human lymphocyte mitogen from wheat germ with N-acetyl-D-glucosamine specificity. J Immunol. 1976 Nov;117(5 PT2):1976–1980. [PubMed] [Google Scholar]
  6. Burger D. R., Vandenbark A. A., Finke P., Nolte J. E., Vetto R. M. Human transfer factor: effects on lymphocyte transformation. J Immunol. 1976 Sep;117(3):782–788. [PubMed] [Google Scholar]
  7. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  8. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens. J Immunol. 1974 Oct;113(4):1113–1121. [PubMed] [Google Scholar]
  9. Ciorbaru R., Adam A., Petit J. F., Lederer E., Bona C., Chedid L. Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae. Infect Immun. 1975 Feb;11(2):257–264. doi: 10.1128/iai.11.2.257-264.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fauci A. S., Pratt K. R. Activation of human B lymphocytes. I. Direct plaque-forming cell assay for the measurement of polyclonal activation and antigenic stimulation of human B lymphocytes. J Exp Med. 1976 Sep 1;144(3):674–684. doi: 10.1084/jem.144.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fauci A. S., Pratt K. R. Polyclonal activation of bone-marrow-derived lymphocytes from human peripheral blood measured by a direct plaque-forming cell assay. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3676–3679. doi: 10.1073/pnas.73.10.3676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. II. Cellular interactions in the PFC response of human tonsillar and peripheral blood B lymphocytes to polyclonal activation by pokeweed mitogen. J Immunol. 1976 Dec;117(6):2100–2104. [PubMed] [Google Scholar]
  13. Goodman G. W., Sultzer B. M. Mild alkaline hydrolysis of lipopolysaccharide endotoxin enhances its mitogencity for murine B cells. Infect Immun. 1977 Jul;17(1):205–214. doi: 10.1128/iai.17.1.205-214.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Greaves M., Janossy G., Doenhoff M. Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens. J Exp Med. 1974 Jul 1;140(1):1–18. doi: 10.1084/jem.140.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gronowicz E., Coutinho A. Functional analysis of B cell heterogeneity. Transplant Rev. 1975;24:3–40. doi: 10.1111/j.1600-065x.1975.tb00164.x. [DOI] [PubMed] [Google Scholar]
  16. Gronowicz E., Coutinho A. Heterogeneity of B cells: direct evidence of selective triggering of distinct subpopulations by polyclonal activators. Scand J Immunol. 1976;5(1-2):55–69. doi: 10.1111/j.1365-3083.1976.tb02992.x. [DOI] [PubMed] [Google Scholar]
  17. Han T., Dadey B. T-lymphocyte dependency of B-lymphocyte blastogenic response to phytomitogens. Immunology. 1978 Apr;34(4):625–629. [PMC free article] [PubMed] [Google Scholar]
  18. Hsu S. H. Blastogenesis of human lymphocytes by endotoxin. Immunol Commun. 1975;4(5):407–417. doi: 10.3109/08820137509057329. [DOI] [PubMed] [Google Scholar]
  19. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Lêthibichthuy, Ciorbaru R., Brochier J. Human B cell differentiation. I. Immunoglobulin synthesis induced by Nocardia mitogen. Eur J Immunol. 1978 Feb;8(2):119–123. doi: 10.1002/eji.1830080209. [DOI] [PubMed] [Google Scholar]
  22. Nilsson B. S., Sultzer B. M., Bullock W. W. Purified protein derivative of tuberculin induces immunoglobulin production in normal mouse spleen cells. J Exp Med. 1973 Jan 1;137(1):127–139. doi: 10.1084/jem.137.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Patters M. R., Genco R. J., Reed M. J., Mashimo P. A. Blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects. Infect Immun. 1976 Nov;14(5):1213–1220. doi: 10.1128/iai.14.5.1213-1220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
  25. Phillips B., Roitt I. M. Evidence for transformation of human B lymphocytes by PHA. Nat New Biol. 1973 Feb 21;241(112):254–256. doi: 10.1038/newbio241254a0. [DOI] [PubMed] [Google Scholar]
  26. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  27. Rodey G. E., Davis T., Quie P. G. Response of peripheral blood lymphocytes from normal, hypogammaglobulinemic and chronic lymphocytic leukemic patients. J Immunol. 1972 Jan;108(1):178–182. [PubMed] [Google Scholar]
  28. Sakane T., Green I. Protein A from Staphylococcus aureus-a mitogen for human T lymphocytes and B lymphocytes but not L lymphocytes. J Immunol. 1978 Jan;120(1):302–311. [PubMed] [Google Scholar]
  29. Schnaitman C. A. Outer membrane proteins of Escherichia coli. IV. Differences in outer membrane proteins due to strain and cultural differences. J Bacteriol. 1974 May;118(2):454–464. doi: 10.1128/jb.118.2.454-464.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sultzer B. M. Genetic factors in leucocyte responses to endotoxin: further studies in mice. J Immunol. 1969 Jul;103(1):32–38. [PubMed] [Google Scholar]
  31. Sultzer B. M., Goodman G. W. Endotoxin protein: a B-cell mitogen and polyclonal activator of C3H/HeJ lymphocytes. J Exp Med. 1976 Sep 1;144(3):821–827. doi: 10.1084/jem.144.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]

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