Abstract
Blood group I activities of the purified glycosphingolipid lacto-N-iso- octaosyl ceramide (Fromula: see text) and 8 of its analogues have been evaluated with 11 anti-I sera including 5 anti-I sera previously tested. All but one of the antisera were inhibited by the lacto-N-iso- octaosyl structure. Three types of I-specificity could be distinguished although none of the anti-I sera was identical in its inhibition patterns with the nine glycophingolipid analogues. The anti-I sera Ma and Woj represent the first type and require an intact Galbeta1 leads to 4GlcNAcbeta1 leads to 6 chain, the anti-I sera Step, Gra, Ver, and Ful represent the second type which requires Galbeta1 leads to 4GlcNAcbeta1 leads to 3 chain with branching, and the anti-I sera Phi, Da, Sch, and Low belong to the third type which requires both branches to be intact. Anti-I antibodies varry in their ability to react with their antigenic determinants in the presence of external substitutions with alpha-linked galactose or sialic acid.
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Selected References
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- Dzierźkowa-Borodej W., Seyfried H., Lisowska E. Serological classification of anti-I sera. Vox Sang. 1975;28(2):110–121. doi: 10.1111/j.1423-0410.1975.tb02749.x. [DOI] [PubMed] [Google Scholar]
- Feizi T., Childs R. A., Hakomori S. I., Powell M. E. Blood-group-Ii-active gangliosides of human erythrocyte membranes. Biochem J. 1978 Jul 1;173(1):245–254. doi: 10.1042/bj1730245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feizi T., Kabat E. A. Immunochemical studies on blood groups. LIV. Classification of anti-I and anti-i sera into groups based on reactivity patterns with various antigens related to the blood group A,B,H, Le a, Le b and precursor substances. J Exp Med. 1972 Jun 1;135(6):1247–1258. doi: 10.1084/jem.135.6.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups. XLVII. The I antigen complex--precursors in the A, B, H, Lea, and leb blood group system--hemagglutination-inhibition studies. J Exp Med. 1971 Jan 1;133(1):39–52. doi: 10.1084/jem.133.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups.XLIX. The I antigen complex: specificity differences among anti-I sera revealed by quantitative precipitin studies; partial structure of the I determinant specific for one anti-I serum. J Immunol. 1971 Jun;106(6):1578–1592. [PubMed] [Google Scholar]
- Feizi T. The I and i antigens on certain normal and pathologic tissues. Rev Fr Transfus Immunohematol. 1978 Feb;21(1):165–174. doi: 10.1016/s0338-4535(78)80039-7. [DOI] [PubMed] [Google Scholar]
- Feizi T., Wood E., Augé C., David S., Veyrières A. Blood group I activities of synthetic oligosaccharides assessed by radioimmunoassay. Immunochemistry. 1978 Nov;15(10-11):733–736. doi: 10.1016/0161-5890(78)90101-3. [DOI] [PubMed] [Google Scholar]
- Gardas A. Studies on the I-blood-group-active sites on macro-glycolipids from human erythrocytes. Eur J Biochem. 1976 Sep;68(1):185–191. doi: 10.1111/j.1432-1033.1976.tb10777.x. [DOI] [PubMed] [Google Scholar]
- Kobata A., Yamashita K., Tachibana Y. Oligosaccharides from human milk. Methods Enzymol. 1978;50:216–220. doi: 10.1016/0076-6879(78)50023-2. [DOI] [PubMed] [Google Scholar]
- Niemann H., Watanabe K., Hakomori S. Blood group i and I activities of "lacto-N-norhexaosylceramide" and its analogues: the structural requirements for i-specificities. Biochem Biophys Res Commun. 1978 Apr 28;81(4):1286–1293. doi: 10.1016/0006-291x(78)91275-5. [DOI] [PubMed] [Google Scholar]
- PAINTER T. J., WATKINS W. M., MORGAN W. T. ISOLATION OF 2 SEROLOGICALLY ACTIVE TRISACCHARIDES FROM HUMAN BLOOD-GROUP B SUBSTANCE. Nature. 1963 Jul 20;199:282–283. doi: 10.1038/199282a0. [DOI] [PubMed] [Google Scholar]
- Pruzanski W., Shumak K. H. Biologic activity of cold-reacting autoantibodies (second of two parts). N Engl J Med. 1977 Sep 15;297(11):583–589. doi: 10.1056/NEJM197709152971105. [DOI] [PubMed] [Google Scholar]
- Watanabe K., Laine R. A., Hakomori S. I. On neutral fucoglycolipids having long, branched carbohydrate chains: H-active and I-active glycosphingolipids of human erythrocyte membranes. Biochemistry. 1975 Jun 17;14(12):2725–2733. doi: 10.1021/bi00683a026. [DOI] [PubMed] [Google Scholar]
- Watanabe K., Powell M., Hakomori S. Isolation and characterization of a novel fucoganglioside of human erythrocyte membranes. J Biol Chem. 1978 Dec 25;253(24):8962–8967. [PubMed] [Google Scholar]