Abstract
The presence of H-2 antigens of the paternal and maternal haplotypes on mouse trophoblast cells was examined at several stages of pregnancy by using a sensitive immunolabeling technique followed by quantitative radioautography. Results revealed the presence of H-2 antigens (determined by the K or D loci) of both parental haplotypes on the F1 trophoblast cells. At 14-16 d of gestation, the antigen density was equivalent to that on adult thymocytes and there was a further 50% increase on day 18. H-2 antigens of both parental haplotypes are also found to be expressed on 11-13 d trophoblast cells.
Full Text
The Full Text of this article is available as a PDF (2.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baines M. G., Speers E. A., Pross H., Millar K. G. Characteristics of the maternal lymphoid response of mice to paternal strain antigens induced by homologous pregnancy. Immunology. 1976 Sep;31(3):363–369. [PMC free article] [PubMed] [Google Scholar]
- Bernard O., Ripoche M. A., Bennett D. Distribution of maternal immunoglobulins in the mouse uterus and embryo in the days after implantation. J Exp Med. 1977 Jan 1;145(1):58–75. doi: 10.1084/jem.145.1.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Billington W. D., Jenkinson E. J., Searle R. F., Sellens M. H. Alloantigen expression during early embryogenesis and placental ontogeny in the mouse: immunoperoxidase and mixed hemadsorption studies. Transplant Proc. 1977 Jun;9(2):1371–1377. [PubMed] [Google Scholar]
- Bulmer D., Peel S. The demonstration of immunoglobulin in the metrial gland cells of the rat placenta. J Reprod Fertil. 1977 Jan;49(1):143–145. doi: 10.1530/jrf.0.0490143. [DOI] [PubMed] [Google Scholar]
- Carter J. Expression of maternal and paternal antigens on trophoblast. Nature. 1976 Jul 22;262(5566):292–293. doi: 10.1038/262292a0. [DOI] [PubMed] [Google Scholar]
- Clark D. A., McDermott M. R. Impairment of host vs graft reaction in pregnant mice. I. Suppression of cytotoxic T cell generation in lymph nodes draining the uterus. J Immunol. 1978 Oct;121(4):1389–1393. [PubMed] [Google Scholar]
- Contractor S. F., Davies H. Effect of human chorionic somatomammotrophin and human chorionic gonadotrophin on phytohaemagglutinin-induced lymphocyte transformation. Nat New Biol. 1973 Jun 27;243(130):284–286. doi: 10.1038/newbio243284a0. [DOI] [PubMed] [Google Scholar]
- Currie G. A., Bagshawe K. D. The masking of antigens on trophoblast and cancer cells. Lancet. 1967 Apr 1;1(7492):708–710. doi: 10.1016/s0140-6736(67)92183-6. [DOI] [PubMed] [Google Scholar]
- Doughty R. W., Gelsthorpe K. An initial investigation of lymphocyte antibody activity through pregnancy and in eluates prepared from placental material. Tissue Antigens. 1974;4(4):291–298. doi: 10.1111/j.1399-0039.1974.tb00255.x. [DOI] [PubMed] [Google Scholar]
- Doughty R. W., Gelsthorpe K. Some parameters of lymphocyte antibody activity through pregnancy and further eluates of placental material. Tissue Antigens. 1976 Jul;8(1):43–48. doi: 10.1111/j.1399-0039.1976.tb00550.x. [DOI] [PubMed] [Google Scholar]
- Faulk W. P., Johnson P. M. Immunological studies of human placentae: identification and distribution of proteins in mature chorionic villi. Clin Exp Immunol. 1977 Feb;27(2):365–375. [PMC free article] [PubMed] [Google Scholar]
- Faulk W. P., Sanderson A. R., Temple A. Distribution of MHC antigens in human placental chorionic villi. Transplant Proc. 1977 Jun;9(2):1379–1384. [PubMed] [Google Scholar]
- Faulk W. P., Temple A., Lovins R. E., Smith N. Antigens of human trophoblasts: a working hypothesis for their role in normal and abnormal pregnancies. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1947–1951. doi: 10.1073/pnas.75.4.1947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garnis S., Lala P. K. Surface markers of small lymphocytes appearing in the mouse Ehrlich ascites tumour, host spleen and blood. Immunology. 1978 Mar;34(3):487–499. [PMC free article] [PubMed] [Google Scholar]
- Goodfellow P. N., Barnstable C. J., Bodmer W. F., Snary D., Crumpton M. J. Expression of HLA system antigens on placenta. Transplantation. 1976 Dec;22(6):595–603. doi: 10.1097/00007890-197612000-00009. [DOI] [PubMed] [Google Scholar]
- Harrison M. R. Maternal immunocompetence. I. The graft-versus-host reactivity of lymphocytes from pregnant rats and the distribution pattern of 51Cr-labeled lymphocytes in pregnant mice. Scand J Immunol. 1976;5(5):549–558. doi: 10.1111/j.1365-3083.1976.tb00310.x. [DOI] [PubMed] [Google Scholar]
- Harrison M. R. Maternal immunocompetence. II. Proliferative responses of maternal lymphocytes in vitro and inhibition by serum from pregnant rats. Scand J Immunol. 1976;5(8):881–889. doi: 10.1111/j.1365-3083.1976.tb03038.x. [DOI] [PubMed] [Google Scholar]
- Hellström I., Hellström K. E. Cytotoxic effect of lymphocytes from pregnant mice on cultivated tumor cells. I. Specificity, nature of effector cells and blocking by serum. Int J Cancer. 1975 Jan 15;15(1):1–16. doi: 10.1002/ijc.2910150102. [DOI] [PubMed] [Google Scholar]
- Håkansson S., Sundqvist K. G. Decreased antigenicity of mouse blastocysts after activation for implantation from experimental delay. Transplantation. 1975 Jun;19(6):479–484. doi: 10.1097/00007890-197506000-00006. [DOI] [PubMed] [Google Scholar]
- KIRBY D. R., BILLINGTON W. D., BRADBURY S., GOLDSTEIN D. J. ANTIGEN BARRIER OF THE MOUSE PLACENTA. Nature. 1964 Nov 7;204:548–549. doi: 10.1038/204548a0. [DOI] [PubMed] [Google Scholar]
- Kanazawa K., Takahashi T., Kajiwara T., Arai S., Ohno M. Studies on a suppressive effect of pregnant plasma on lymphocyte response to phytohemagglutinin (PHA) in vitro. Acta Obstet Gynaecol Jpn. 1974 Jul;21(3):143–146. [PubMed] [Google Scholar]
- Kirby D. R. The immunological consequences of extrauterine development of allogeneic mouse blastocysts. Transplantation. 1968 Dec;6(9):1005–1009. doi: 10.1097/00007890-196812000-00007. [DOI] [PubMed] [Google Scholar]
- Lala P. K., Patt H. M. Cytokinetic analysis of tumor growth. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1735–1742. doi: 10.1073/pnas.56.6.1735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murgita R. A., Goidl E. A., Kontianen S., Wigzell H. alpha-Fetoprotein induces suppressor T cells in vitro. Nature. 1977 May 19;267(5608):257–259. doi: 10.1038/267257a0. [DOI] [PubMed] [Google Scholar]
- Rees R. C., Bray J., Robins R. A., Baldwin R. W. Subpopulations of multiparous rat lymph-node cells cytotoxic for rat tumour cells and capable of suppressing cytotoxicity in vitro. Int J Cancer. 1975 May 15;15(5):762–772. doi: 10.1002/ijc.2910150507. [DOI] [PubMed] [Google Scholar]
- SIMMONS R. L., RUSSELL P. S. The antigenicity of mouse trophoblast. Ann N Y Acad Sci. 1962 Oct 24;99:717–732. doi: 10.1111/j.1749-6632.1962.tb45356.x. [DOI] [PubMed] [Google Scholar]
- Sundqvist K. G., Bergström S., Håkansson S. Surface antigens of human trophoblasts. Dev Comp Immunol. 1977 Jul;1(3):241–254. doi: 10.1016/s0145-305x(77)80033-5. [DOI] [PubMed] [Google Scholar]
- Svehag S. E., Schilling W. Immunosuppressive effect of a mouse placenta fraction on H-2 incompatible split heart allografts. Experientia. 1976 Sep 15;32(9):1201–1202. doi: 10.1007/BF01927626. [DOI] [PubMed] [Google Scholar]
- Taylor P. V., Hancock K. W. Antigenicity of trophoblast and possible antigen-masking effects during pregnancy. Immunology. 1975 May;28(5):973–982. [PMC free article] [PubMed] [Google Scholar]
- Tongio M. M., Mayer S. Narrowing of feto-maternal immunization at time of delivery. Tissue Antigens. 1977 Mar;9(3):174–176. doi: 10.1111/j.1399-0039.1977.tb01102.x. [DOI] [PubMed] [Google Scholar]
- Wegmann T. G., Singh B., Carlson G. A. Allogeneic placenta is a paternal strain antigen immunoabsorbent. J Immunol. 1979 Jan;122(1):270–274. [PubMed] [Google Scholar]
