Abstract
We investigated T-T cell interactions in the suppression of contact sensitivity. Suppressor cells that block the efferent limb of sensitivity (Ts-eff) can inhibit the passive transfer of contact sensitivity mediated by 1-fluoro-2,4-dinitrobenzene immune cells (T DH). But, Ts-eff cannot block the passive transfer of TDH which comes from cyclophosphamide (Cy) pretreated sensitized mice. We interpret these results to indicate that lymph node cells from sensitized mice contain not only TDH but also another intermediate cell which is required for the suppression of TDH by Ts-eff. This intermediate cell is sensitive to cyclophosphamide and requires antigen activation for its development. It is sensitive to adult thymectomy and anti-brain associated theta serum and is therefore designated as an auxiliary T- suppressor cell (Ts-aux). It is not sensitive to splenectomy and it carries I-J determinants. Ts-aux are required for the activity of suppressors of the efferent limb (Ts-eff) but not of suppressors of the afferent limb (Ts-aff). Thus, in the feedback loops in contact sensitivity, the generation of Tdh is coordinated with the development of auxiliary Ts which are essential for the suppression of those TDH.
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Selected References
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- Asherson G. L., Barnes R. M. Contact sensitivity in the mouse. XII. The use of DNA synthesis in vivo to determine the anatomical location of immunological unresponsiveness to picryl chloride. Immunology. 1973 Sep;25(3):495–508. [PMC free article] [PubMed] [Google Scholar]
- Bach F. H., Widmer M. B., Segall M., Bach M. L., Klein J. Genetic and immunological complexity of major histocompatibility regions. Science. 1972 Jun 2;176(4038):1024–1027. doi: 10.1126/science.176.4038.1024. [DOI] [PubMed] [Google Scholar]
- Bullock W. W., Katz D. H., Benacerraf B. Induction of T-lymphocyte responses to a small molecular weight antigen. III. T-T cell interactions to determinants linked together: suppression vs. enhancement. J Exp Med. 1975 Aug 1;142(2):275–287. doi: 10.1084/jem.142.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. 3. Evidence for interaction between two types of thymus-derived cells. J Exp Med. 1972 Apr 1;135(4):764–779. doi: 10.1084/jem.135.4.764. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feldmann M., Kilburn D. G., Levy J. T-T interaction in the generation of helper cells in vitro. Nature. 1975 Aug 28;256(5520):741–743. doi: 10.1038/256741a0. [DOI] [PubMed] [Google Scholar]
- Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
- Howe M. L., Cohen L. Lymphoid cell subpopulations. I. Synergy between lymph node cells and thymocytes in response to alloantigens and mitogens. J Immunol. 1975 Nov;115(5):1227–1232. [PubMed] [Google Scholar]
- Miller S. D., Sy M. S., Claman H. N. Suppressor T cell mechanisms in contact sensitivity. I. Efferent blockade by syninduced suppressor T cells. J Immunol. 1978 Jul;121(1):265–273. [PubMed] [Google Scholar]
- Moorhead J. W. Tolerance and contact sensitivity to DNFA in mice. VIII. Identification of distinct T cell subpopulations that mediate in vivo and in vitro manifestations of delayed hypersensitivity. J Immunol. 1978 Jan;120(1):137–144. [PubMed] [Google Scholar]
- Moorhead J. W. Tolerance and contact sensitivity to DNFB in mice. VI. Inhibition of afferent sensitivity by suppressor T cells in adoptive tolerance. J Immunol. 1976 Sep;117(3):802–806. [PubMed] [Google Scholar]
- Moorhead J. W., Walters C. S., Claman H. N. Immunologic reactions to haptens on autologous carriers. I. Participation of both thymus-derived and bone marrow-derived cells in the secondary in vitro response. J Exp Med. 1973 Feb 1;137(2):411–423. doi: 10.1084/jem.137.2.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niederhuber J. E., Frelinger J. A., Dine M. S., Shoffner P., Dugan E., Shreffler D. C. Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells. J Exp Med. 1976 Feb 1;143(2):372–381. doi: 10.1084/jem.143.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osborne D. P., Jr, Katz D. H. The allogeneic effect in inbred mice. IV. Regulatory influences of graft-vs.-host reactions on host T lymphocyte functions. J Exp Med. 1973 Oct 1;138(4):825–838. doi: 10.1084/jem.138.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sy M. S., Miller S. D., Kowach H. B., Claman H. N. A splenic requirement for the generation of suppressor T cells. J Immunol. 1977 Dec;119(6):2095–2099. [PubMed] [Google Scholar]
- Wagner H. Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973 Dec 1;138(6):1379–1397. doi: 10.1084/jem.138.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]