Abstract
Lewis rats were injected intravenously with rabbit anti-rat glomerular basement membrane (GBM) antisera in doses that were sufficient to cause glomerular fixation of rabbit gamma globulin (RGG) detectable by immunofluorescence, but which failed to induce histologically detectable lesions. 24 h later, groups of rats received lymph node cells or serum from syngeneic donors that had been immunized with either RGG or ovalbumin; they were injected with [3H]thymidine three times during the next 2 days, and sacrificed 48 or 96 h after transfer. Only the rats given anti-GBM antiserum plus lymph node cells from donors sensitized to RGG showed histological glomerular lesions, in the form of segmental hypercellularly and necrosis. Autoradiographs revealed the greatest number of labeled cells in glomeruli in the same group. In analogous experiments, it was shown that T-cell-enriched populations could induce hypercellular glomerular reactions. On the basis of electronmicroscopic and autoradiographic observations, it appears that the glomerular hypercellularity resulted from both infiltration of mononuclear cells and proliferation of endothelial cells. The findings indicate that interaction of specifically sensitized lymphocytes with glomerular-bound antigen can induce a cell- mediated (delayed-type) reaction in glomeruli.
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- Andres G. A., Cerra F., Elti G., Casciani C., Cortesini R., Hsu K. C. Lymphocyte and blast cell glomerulonephritis in renal allografts of rats injected with phytohaemagglutinin. Cell Immunol. 1974 Jul;13(1):146–163. doi: 10.1016/0008-8749(74)90234-2. [DOI] [PubMed] [Google Scholar]
- Dixon F. J. What are sensitized cells doing in glomerulonephritis? N Engl J Med. 1970 Sep 3;283(10):536–537. doi: 10.1056/NEJM197009032831011. [DOI] [PubMed] [Google Scholar]
- Dvorak A. M., Mihm M. C., Jr, Dvorak H. F. Morphology of delayed-type hypersensitivity reactions in man. II. Ultrastructural alterations affecting the microvasculature and the tissue mast cells. Lab Invest. 1976 Feb;34(2):179–191. [PubMed] [Google Scholar]
- Johnston W. H., Latta H. Acute focal glomerulonephritis in the rabbit induced by injection of Saccharomyces cerevisiae. An electron microscopic study. Lab Invest. 1972 Jun;26(6):741–754. [PubMed] [Google Scholar]
- KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
- Kondo Y., Shigematsu H., Kobayashi Y. Cellular aspects of rabbit Masugi nephritis. II. Progressive glomerular injuries with crescent formation. Lab Invest. 1972 Dec;27(6):620–631. [PubMed] [Google Scholar]
- Kühn K., Ryan G. B., Hein S. J., Galaske R. G., Karnovsky M. J. An ultrastructural study of the mechanisms of proteinuria in rat nephrotoxic nephritis. Lab Invest. 1977 Apr;36(4):375–387. [PubMed] [Google Scholar]
- Mahieu P., Dardenne M., Bach J. F. Detection of humoral and cell-mediated immunity to kidney basement membranes in human renal diseases. Am J Med. 1972 Aug;53(2):185–192. doi: 10.1016/0002-9343(72)90128-3. [DOI] [PubMed] [Google Scholar]
- Polverini P. J., Cotran R. S., Sholley M. M. Endothelial proliferation in the delayed hypersensitivity reaction: an autoradiographic study. J Immunol. 1977 Feb;118(2):529–532. [PubMed] [Google Scholar]
- Rocklin R. E., Lewis E. J., David J. R. In vitro evidence for cellular hypersensitivity to glomerular-basement-membrane antigens in human glomerulonephritis. N Engl J Med. 1970 Sep 3;283(10):497–501. doi: 10.1056/NEJM197009032831001. [DOI] [PubMed] [Google Scholar]
- Schantz A., Schecter A. Iron-hematoxylin and safranin O as a polychrome stain for epon sections. Stain Technol. 1965 Sep;40(5):279–282. doi: 10.3109/10520296509116427. [DOI] [PubMed] [Google Scholar]
- Schneeberger E. E., Collins A. B., Latta H., McCluskey R. T. Diminished glomerular accumulation of colloidal carbon in autologous immune complex nephritis. Lab Invest. 1977 Jul;37(1):9–19. [PubMed] [Google Scholar]
- Schreiner G. F., Cotran R. S., Pardo V., Unanue E. R. A mononuclear cell component in experimental immunological glomerulonephritis. J Exp Med. 1978 Feb 1;147(2):369–384. doi: 10.1084/jem.147.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shigematsu H. Glomerular events during the initial phase of rat Masugi nephritis. Virchows Arch B Cell Pathol. 1970;5(3):187–200. [PubMed] [Google Scholar]
- UNANUE E. R., DIXON F. J. EXPERIMENTAL GLOMERULONEPHRITIS. VI. THE AUTOLOGOUS PHASE OF NEPHROTOXIC SERUM NEPHRITIS. J Exp Med. 1965 May 1;121:715–725. doi: 10.1084/jem.121.5.715. [DOI] [PMC free article] [PubMed] [Google Scholar]