Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1978 Oct 1;148(4):953–962. doi: 10.1084/jem.148.4.953

In vitro sensitization of thymocytes. Role of H-21 I region determinants and cell-free mixed leukocyte culture supernates in generation of cytotoxic responses

PMCID: PMC2185020  PMID: 100573

Abstract

Stimulation of thymocytes in vitro by spleen cells differing for the entire H-2 complex leads to a significant proliferative response without a significant cell-mediated lympholysis (CML) response. Addition of soluble cell-free supernates (SF), (taken from a 7-day mixed leukocyte culture) enables these cultures to develop CML response. For optimal CML response, the SF has to be added within 48 h of onset of cultures. Although with spleen cells as responding cells, SF could quantitatively replace I-region different stimulating cells for generation of CML responses, with thymocytes as responding cells, stimulation with I-region cells appeared obligatory for the generation of CML responses. The implications of these findings are discussed.

Full Text

The Full Text of this article is available as a PDF (571.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman A., Cohen I. R. Cell-free media of mixed lymphocyte cultures augmenting sensitization in vitro of mouse T lymphocytes against allogeneic fibroblasts. Eur J Immunol. 1976 Jul;5(7):437–444. doi: 10.1002/eji.1830050702. [DOI] [PubMed] [Google Scholar]
  2. Bach F. H., Bach M. L., Sondel P. M. Differential function of major histocompatibility complex antigens in T-lymphocyte activation. Nature. 1976 Jan 29;259(5541):273–281. doi: 10.1038/259273a0. [DOI] [PubMed] [Google Scholar]
  3. Bach F. H., Grillot-Courvalin C., Kuperman O. J., Sollinger H. W., Hayes C., Sondel P. M., Alter B. J., Bach M. L. Antigenic requirements for triggering of cytotoxic T lymphocytes. Immunol Rev. 1977;35:76–96. doi: 10.1111/j.1600-065x.1977.tb00236.x. [DOI] [PubMed] [Google Scholar]
  4. Bach F. H., Kuperman O. J., Sollinger H. W., Zarling J. M., Sondel P. M., Alter B. J., Bach M. L. Cellular immunogenetics and LD-CD collaboration. Transplant Proc. 1977 Mar;9(1):859–863. [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farrar J. J. The xenogeneic effect. I. Antigen and mitogen-stimulated human lymphocytes produce a non-antigen-specific factor which reconstitutes the antibody response of T cell-deficient mouse spleen cells. J Immunol. 1975 Nov;115(5):1295–1300. [PubMed] [Google Scholar]
  7. Klein J., Forman J., Hauptfeld V., Egorov I. K. Immunogenetic analysis of H-2 mutations. III. Genetic mapping and involvement in immune reactions of the H-2ka mutation. J Immunol. 1975 Sep;115(3):716–718. [PubMed] [Google Scholar]
  8. Kuperman O. J., Bach F. H. Genetic basis of memory in cell-mediated immune response. Scand J Immunol. 1977;6(3):161–172. doi: 10.1111/j.1365-3083.1977.tb00381.x. [DOI] [PubMed] [Google Scholar]
  9. Peck A. B., Bach F. H. Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors. Scand J Immunol. 1975;4(1):53–62. doi: 10.1111/j.1365-3083.1975.tb02599.x. [DOI] [PubMed] [Google Scholar]
  10. Pilarski L. M. Ontogeny of cell-mediated immunity. I. Early development of alloantigen-specific cytotoxic T-cell precursors in postnatal mice. J Exp Med. 1977 Sep 1;146(3):887–892. doi: 10.1084/jem.146.3.887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Plate J. M. Soluble factors substitute for T-T-cell collaboration in generation of T-killer lymphocytes. Nature. 1976 Mar 25;260(5549):329–331. doi: 10.1038/260329a0. [DOI] [PubMed] [Google Scholar]
  12. Schendel D. J., Alter B. J., Bach F. H. The involvement of LD- and SD-region differences in MLC and CML: a three-cell experiment. Transplant Proc. 1973 Dec;5(4):1651–1655. [PubMed] [Google Scholar]
  13. Simon P. L., Farrar J. J., Kind P. D. The xenogeneic effect. III. Induction of cell-mediated cytotoxicity by alloantigen-stimulated thymocytes in the presence of xenogeneic reconstitution factor. J Immunol. 1977 Apr;118(4):1129–1131. [PubMed] [Google Scholar]
  14. Sondel P. M., Jacobson M. W., Bach F. H. Selective activation of human suppressor cells by a nonproliferative stimulus. Eur J Immunol. 1977 Jan;7(1):38–40. doi: 10.1002/eji.1830070109. [DOI] [PubMed] [Google Scholar]
  15. Sopori M. L., Alter B. J., Bach F. H., Bach M. L. Cell-free factor substitute for "signal 2" in generating cytotoxic reactions. Eur J Immunol. 1977 Nov;7(11):823–825. doi: 10.1002/eji.1830071115. [DOI] [PubMed] [Google Scholar]
  16. Wagner H. Synergy during in vitro cytotoxic allograft responses. I. Evidence for cell interaction between thymocytes and peripheral T cells. J Exp Med. 1973 Dec 1;138(6):1379–1397. doi: 10.1084/jem.138.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES