Abstract
Epithelial and mesenchymal cells synthesized and secreted all three subcomponents of the first component of complement (C1): C1q, C1r, and C1s. Quantitatively, however, columnar and transitional epithelial cells secreted 400--3,700 times more hemolytically active C1 than monocytes or fibroblasts. Only columnar epithelial cells synthesized C1 subcomponents with subunit structures similar to their serum counterparts. Transitional epithelial cells, fibroblasts, and monocytes produced C1q and C1s with subunits of apparent molecular weights larger than reported values. C1r from all cell lines was physiochemically similar to serum C1r.
Full Text
The Full Text of this article is available as a PDF (837.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Al-Adnani M. S., McGee J. O. Clq production and secretion by fibroblasts. Nature. 1976 Sep 9;263(5573):145–146. doi: 10.1038/263145a0. [DOI] [PubMed] [Google Scholar]
- Assimeh S. N., Bing D. H., Painter R. H. A simple method for the isolation of the subcomponents of the first component of complement by affinity chromatography. J Immunol. 1974 Jul;113(1):225–234. [PubMed] [Google Scholar]
- BORSOS T., RAPP H. J. CHROMATOGRAPHIC SEPARATION OF THE FIRST COMPONENT OF COMPLEMENT AND ITS ASSAY ON A MOLECULAR BASIS. J Immunol. 1963 Dec;91:851–858. [PubMed] [Google Scholar]
- Bing D. H., Spurlock S. E., Bern M. M. Synthesis of the first component of complement by primary cultures of human tumors of the colon and urogenital tract and comparable normal tissue. Clin Immunol Immunopathol. 1975 Sep;4(3):341–351. doi: 10.1016/0090-1229(75)90003-3. [DOI] [PubMed] [Google Scholar]
- Colten H. R., Borsos T., Rapp H. J. In vitro synthesis of the first component of complement by guinea pig small intestine. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1158–1163. doi: 10.1073/pnas.56.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colten H. R., Gordon J. M., Borsos T., Rapp H. J. Synthesis of the first component of human complement in vitro. J Exp Med. 1968 Oct 1;128(4):595–604. doi: 10.1084/jem.128.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colten H. R., Gordon J. M., Rapp H. J., Borsos T. Synthesis of the first component of guinea pig complement by columnar epithelial cells of the small intestine. J Immunol. 1968 Apr;100(4):788–792. [PubMed] [Google Scholar]
- Day N. K., Gewurz H., Pickering R. J., Good R. A. Ontogenetic development of C1q synthesis in the piglet. J Immunol. 1970 May;104(5):1316–1319. [PubMed] [Google Scholar]
- Einstein L. P., Schneeberger E. E., Colten H. R. Synthesis of the second component of complement by long-term primary cultures of human monocytes. J Exp Med. 1976 Jan 1;143(1):114–126. doi: 10.1084/jem.143.1.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall R. E., Colten H. R. Cell-free synthesis of the fourth component of guinea pig complement (C4): identification of a precursor of serum C4 (pro-C4). Proc Natl Acad Sci U S A. 1977 Apr;74(4):1707–1710. doi: 10.1073/pnas.74.4.1707. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall R. E., Colten H. R. Molecular size and subunit structure of the fourth component of guinea pig complement. J Immunol. 1977 May;118(5):1903–1905. [PubMed] [Google Scholar]
- Ishizaka T., Ishizaka K., Borsos T., Rapp H. C'-1 fixation by human isoagglutinins: fixation of C'-1 by gamma-G and gamma-M but not by gamma-A antibody. J Immunol. 1966 Nov;97(5):716–726. [PubMed] [Google Scholar]
- Kohler P. F. Maturation of the human complement system. I. Onset time and sites of fetal C1q, C4, C3, and C5 synthesis. J Clin Invest. 1973 Mar;52(3):671–677. doi: 10.1172/JCI107228. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEPOW I. H., NAFF G. B., TODD E. W., PENSKY J., HINZ C. F. Chromatographic resolution of the first component of human complement into three activities. J Exp Med. 1963 Jun 1;117:983–1008. doi: 10.1084/jem.117.6.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owens R. B., Smith H. S., Nelson-Rees W. A., Springer E. L. Epithelial cell cultures from normal and cancerous human tissues. J Natl Cancer Inst. 1976 Apr;56(4):843–849. doi: 10.1093/jnci/56.4.843. [DOI] [PubMed] [Google Scholar]
- Reid K. B. Isolation, by partial pepsin digestion, of the three collagen-like regions present in subcomponent Clq of the first component of human complement. Biochem J. 1976 Apr 1;155(1):5–17. doi: 10.1042/bj1550005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reid K. B., Porter R. R. Subunit composition and structure of subcomponent C1q of the first component of human complement. Biochem J. 1976 Apr 1;155(1):19–23. doi: 10.1042/bj1550019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reid K. B., Sim R. B., Faiers A. P. Inhibition of the reconstitution of the haemolytic activity of the first component of human complement by a pepsin-derived fragment of subcomponent C1q. Biochem J. 1977 Feb 1;161(2):239–245. doi: 10.1042/bj1610239. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reid K. B., Solomon E. Biosynthesis of the first component of complement by human fibroblasts. Biochem J. 1977 Dec 1;167(3):647–660. doi: 10.1042/bj1670647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sim R. B., Porter R. R., Reid K. B., Gigli I. The structure and enzymic activities of the C1r and C1s subcomponents of C1, the first component of human serum complement. Biochem J. 1977 May 1;163(2):219–227. doi: 10.1042/bj1630219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stecher V. J., Morse J. H., Thorbecke G. J. Sites of production of primate serum proteins associated with complement system. Proc Soc Exp Biol Med. 1967 Feb;124(2):433–438. doi: 10.3181/00379727-124-31758. [DOI] [PubMed] [Google Scholar]
- Ziccardi R. J., Cooper N. R. Activation of C1r by proteolytic cleavage. J Immunol. 1976 Feb;116(2):504–509. [PubMed] [Google Scholar]
- Ziccardi R. J., Cooper N. R. Physicochemical and functional characterization of the C1r subunit of the first complement component. J Immunol. 1976 Feb;116(2):496–503. [PubMed] [Google Scholar]
- von Zeipel G., Sjöholm A., Laurell A. B. Analysis of C1 subcomponents in cell culture media of HeLa cells and diploid human fetal lung fibroblasts. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Apr;81(2):259–263. doi: 10.1111/j.1699-0463.1973.tb00220.x. [DOI] [PubMed] [Google Scholar]