Abstract
A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.
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Selected References
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- Andersson J., Coutinho A., Lernhardt W., Melchers F. Clonal growth and maturation to immunoglobulin secretion in vitro of every growth-inducible B lymphocyte. Cell. 1977 Jan;10(1):27–34. doi: 10.1016/0092-8674(77)90136-2. [DOI] [PubMed] [Google Scholar]
- Andersson J., Coutinho A., Melchers F. Frequencies of mitogen-reactive B cells in the mouse. II. Frequencies of B cells producing antibodies which lyse sheep or horse erythrocytes, and trinitrophenylated or nitroiodophenylated sheep erythrocytes. J Exp Med. 1977 Jun 1;145(6):1520–1530. doi: 10.1084/jem.145.6.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan E. L., Henry C. Coexistence of helper and suppressor activities in carrier-primed spleen cells. J Immunol. 1976 Oct;117(4):1132–1138. [PubMed] [Google Scholar]
- Guilbert L. J., Iscove N. N. Partial replacement of serum by selenite, transferrin, albumin and lecithin in haemopoietic cell cultures. Nature. 1976 Oct 14;263(5578):594–595. doi: 10.1038/263594a0. [DOI] [PubMed] [Google Scholar]
- Hartmann K. U. Induction of a hemolysin response in vitro. Interaction of cells of bone marrow origin and thymic origin. J Exp Med. 1970 Dec 1;132(6):1267–1278. doi: 10.1084/jem.132.6.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iscove N. N., Melchers F. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1;147(3):923–933. doi: 10.1084/jem.147.3.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz D. H., Paul W. E., Goidl E. A., Benacerraf B. Radioresistance of cooperative function of carrier-specific lymphocytes in antihapten antibody responses. Science. 1970 Oct 23;170(3956):462–464. doi: 10.1126/science.170.3956.462. [DOI] [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. The thymus and the precursors of antigen reactive cells. Nature. 1967 Nov 18;216(5116):659–663. doi: 10.1038/216659a0. [DOI] [PubMed] [Google Scholar]
- Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osmond D. G., Nossal G. J. Differentiation of lymphocytes in mouse bone marrow. I. Quantitative radioautographic studies of antiglobulin binding by lymphocytes in bone marrow and lymphoid tissues. Cell Immunol. 1974 Jul;13(1):117–131. doi: 10.1016/0008-8749(74)90232-9. [DOI] [PubMed] [Google Scholar]
- Quintáns J., Lefkovits I. Precursor cells specific to sheep red cells in nude mice. Estimation of frequency in the microculture system. Eur J Immunol. 1973 Jul;3(7):392–397. doi: 10.1002/eji.1830030704. [DOI] [PubMed] [Google Scholar]