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. 1978 Dec;136(3):833–838. doi: 10.1128/jb.136.3.833-838.1978

Cell fractions and enzymatic activities of Ureaplasma urealyticum.

N Romano, R La Licata
PMCID: PMC218515  PMID: 214422

Abstract

The localization of some enzymic activities in cell fractions of Ureaplasma urealyticum was studied. A quantitative evaluation of the effectiveness of several cell lysis procedures was obtained by using labeled membranes and sucrose density gradient centrifugation. Ultrasonic treatment was found to be the most effective procedure for lysing the cells, whereas digitonin and osmotic shock caused the lysis of only 70 and 50% of the cells, respectively. The localization of selected enzymes in Ureaplasma cells resembled that found in other Mycoplasma species. Adenosine triphosphatase, ribonuclease, deoxyribonuclease, and p-nitrophenylphosphatase activities were located exclusively in the membrane fraction, whereas urease and L-histidine ammonia-lyase were located in the cytoplasm.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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