Abstract
The specificity of nucleoside uptake in germinating conidia of Neurospora crassa was investigated by examining the kinetics of [2-14C]uridine and [8-14C]-adenosine uptake in the wild-type, ad-8, and ud-1 pyr-1 strains. The results obtained strongly indicate that nucleoside transport in N. crassa is mediated solely by a general transport system which accepts both purine and pyrimidine nucleosides. Studies directed at characterizing the specificity of the transport system indicate that general structural features of the nucleoside which enhance its efficiency in binding to the transport system include: (i) a purine or pyrimidine as the heterocyclic ring, (ii) an unfunctionalized ribose or 2'-deoxyribose as the sugar unit, (iii) a beta-configuration about the anomeric carbon, (iv) the absence of substituents at C8 in the purine series and at C5 and C6 in the pyrimidine series, (v) the presence of a C5-C6 double bond in the pyrimidine series, and (vi) the absence of a charge on the heterocyclic ring.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berlin R. D., Oliver J. M. Membrane transport of purine and pyrimidine bases and nucleosides in animal cells. Int Rev Cytol. 1975;42:287–336. doi: 10.1016/s0074-7696(08)60983-3. [DOI] [PubMed] [Google Scholar]
- Cass C. E., Paterson A. R. Mediated transport of nucleosides by human erythrocytes. Specificity toward purine nucleosides as permeants. Biochim Biophys Acta. 1973 Feb 16;291(3):734–746. doi: 10.1016/0005-2736(73)90477-x. [DOI] [PubMed] [Google Scholar]
- Cass C. E., Paterson A. R. Mediated transport of nucleosides in human erythrocytes. Accelerative exchange diffusion of uridine and thymidine and specificity toward pyrimidine nucleosides as permeants. J Biol Chem. 1972 May 25;247(10):3314–3320. [PubMed] [Google Scholar]
- Magill J. M., Spencer R. R., Magill C. W. Relationship between (8-14C)adenosine transport and growth inhibition in Neurospora crassa strain ad-8. J Bacteriol. 1974 Jul;119(1):202–206. doi: 10.1128/jb.119.1.202-206.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Paterson A. R., Kim S. C., Bernard O., Cass C. E. Transport of nucleosides. Ann N Y Acad Sci. 1975 Aug 8;255:402–411. doi: 10.1111/j.1749-6632.1975.tb29248.x. [DOI] [PubMed] [Google Scholar]
- Rohrer D. C., Sundaralingam M. Stereochemistry of nucleic acids and their constituents. VI. The crystal structure and conformation of dihydrouracil: a minor base of transfer-ribonucleic acid. Acta Crystallogr B. 1970 May 15;26(5):546–553. doi: 10.1107/s0567740870002789. [DOI] [PubMed] [Google Scholar]
- Schiltz J. R., Terry K. D. Nucleoside uptake during the germination of Neurospora crassa conidia. Biochim Biophys Acta. 1970;209(2):278–288. doi: 10.1016/0005-2787(70)90726-4. [DOI] [PubMed] [Google Scholar]
- Schweizer M. P., Witkowski J. T., Robins R. K. Nuclear magnetic resonance determination of syn and anti conformations in pyrimidine nucleosides. J Am Chem Soc. 1971 Jan 13;93(1):277–279. doi: 10.1021/ja00730a062. [DOI] [PubMed] [Google Scholar]
- Taube R. A., Berlin R. D. Membrane transport of nucleosides in rabbit polymorphonuclear leukocytes. Biochim Biophys Acta. 1972 Jan 17;255(1):6–18. doi: 10.1016/0005-2736(72)90003-x. [DOI] [PubMed] [Google Scholar]
- Tavale S. S., Sobell H. M. Crystal and molecular structure of 8-bromoguanosine and 8-bromoadenosine, two purine nucleosides in the syn conformation. J Mol Biol. 1970 Feb 28;48(1):109–123. doi: 10.1016/0022-2836(70)90222-6. [DOI] [PubMed] [Google Scholar]
- Williams L. G., Mitchell H. K. Mutants affecting thymidine metabolism in Neurospora crassa. J Bacteriol. 1969 Oct;100(1):383–389. doi: 10.1128/jb.100.1.383-389.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]