Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Dec;136(3):1189–1191. doi: 10.1128/jb.136.3.1189-1191.1978

Bypass of receptor-mediated resistance to colicin E3 in Escherichia coli K-12.

M Tilby, I Hindennach, U Henning
PMCID: PMC218557  PMID: 363693

Abstract

Colicin E3 was found to kill, under conditions of osmotic shock, cells lacking a functional outer membrane receptor (bfe). Under such conditions, component A of the colicin, carrying endonucleolytic activity, also killed bfe cells, whereas fragment T2, obtained by tryptic digestion of the colicin and also active endonucleolytically, was inactive. Tolerance to the colicin caused by defects in the outer membrane could be overcome by osmotic shock, whereas tolerance probably caused by an altered plasma membrane could not.

Full text

PDF
1189

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassford P. J., Jr, Kadner R. J., Schnaitman C. A. Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles. J Bacteriol. 1977 Jan;129(1):265–275. doi: 10.1128/jb.129.1.265-275.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassford P. J., Jr, Schnaitman C. A., Kadner R. J. Functional stability of the bfe and tonB gene products in Escherichia coli. J Bacteriol. 1977 May;130(2):750–758. doi: 10.1128/jb.130.2.750-758.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bayer M. E. Adsorption of bacteriophages to adhesions between wall and membrane of Escherichia coli. J Virol. 1968 Apr;2(4):346–356. doi: 10.1128/jvi.2.4.346-356.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bayer M. E. Areas of adhesion between wall and membrane of Escherichia coli. J Gen Microbiol. 1968 Oct;53(3):395–404. doi: 10.1099/00221287-53-3-395. [DOI] [PubMed] [Google Scholar]
  5. Bhattacharyya P., Wendt L., Whitney E., Silver S. Colicin-tolerant mutants of Escherichia coli: resistance of membranes to colicin E1. Science. 1970 May 22;168(3934):998–1000. doi: 10.1126/science.168.3934.998. [DOI] [PubMed] [Google Scholar]
  6. Boon T. Inactivation of ribosomes in vitro by colicin E 3 . Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421–2425. doi: 10.1073/pnas.68.10.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bowman C. M., Dahlberg J. E., Ikemura T., Konisky J., Nomura M. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Proc Natl Acad Sci U S A. 1971 May;68(5):964–968. doi: 10.1073/pnas.68.5.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bowman C. M., Sidikaro J., Nomura M. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells. Nat New Biol. 1971 Dec 1;234(48):133–137. doi: 10.1038/newbio234133a0. [DOI] [PubMed] [Google Scholar]
  9. Chai T. J., Foulds J. Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane. J Bacteriol. 1977 May;130(2):781–786. doi: 10.1128/jb.130.2.781-786.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Datta D. B., Arden B., Henning U. Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors. J Bacteriol. 1977 Sep;131(3):821–829. doi: 10.1128/jb.131.3.821-829.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Davies J. K., Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. J Bacteriol. 1975 Jul;123(1):102–117. doi: 10.1128/jb.123.1.102-117.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Foulds J., Chai T. J. Defeat of colicin tolerance in Escherichia coli ompA mutants: evidence for interaction between colicin L-JF246 and the cytoplasmic membrane. J Bacteriol. 1978 Jan;133(1):158–164. doi: 10.1128/jb.133.1.158-164.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Foulds J. Purification and partial characterization of a bacteriocin from Serratia marcescens. J Bacteriol. 1972 Jun;110(3):1001–1009. doi: 10.1128/jb.110.3.1001-1009.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Glick J. M., Kerr S. J., Gold A. M., Shemin D. Multiple forms of colicin E 3 from Escherichia coli CA-38 (col 3 , col I). Biochemistry. 1972 Mar 28;11(7):1183–1188. doi: 10.1021/bi00757a011. [DOI] [PubMed] [Google Scholar]
  15. Herschman H. R., Helinski D. R. Purification and characterization of colicin E2 and colicin E3. J Biol Chem. 1967 Nov 25;242(22):5360–5368. [PubMed] [Google Scholar]
  16. Jakes K. S., Zinder N. D. Highly purified colicin E3 contains immunity protein. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3380–3384. doi: 10.1073/pnas.71.9.3380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jetten A. M., Jetten M. E. Energy requirement for the initiation of colicin action in Escherichia coli. Biochim Biophys Acta. 1975 Apr 14;387(1):12–22. doi: 10.1016/0005-2728(75)90048-1. [DOI] [PubMed] [Google Scholar]
  18. Lau C., Richards F. M. Proteolytic and chemical modification of colicin E3 activity. Biochemistry. 1976 Aug 24;15(17):3856–3863. doi: 10.1021/bi00662a032. [DOI] [PubMed] [Google Scholar]
  19. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  20. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  21. Ohno S., Ohno-Iwashita Y., Suzuki K., Imahori K. Purification and characterization of active component and active fragment of colicin E3. J Biochem. 1977 Oct;82(4):1045–1053. doi: 10.1093/oxfordjournals.jbchem.a131775. [DOI] [PubMed] [Google Scholar]
  22. Sabet S. F., Schnaitman C. A. Purification and properties of the colicin E3 receptor of Escherichia coli. J Biol Chem. 1973 Mar 10;248(5):1797–1806. [PubMed] [Google Scholar]
  23. Schmitges C. J., Henning U. The major proteins of the Escherichia coli outer cell-envelope membrane. Heterogeneity of protein I. Eur J Biochem. 1976 Mar 16;63(1):47–52. doi: 10.1111/j.1432-1033.1976.tb10205.x. [DOI] [PubMed] [Google Scholar]
  24. Senior B. W., Holland I. B. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli. Proc Natl Acad Sci U S A. 1971 May;68(5):959–963. doi: 10.1073/pnas.68.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Skurray R. A., Hancock R. E., Reeves P. Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage. J Bacteriol. 1974 Sep;119(3):726–735. doi: 10.1128/jb.119.3.726-735.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Takagaki Y., Kunugita K., Matsuhashi M. Evidence for the direct action of colicin K on aerobic 32 P i uptake in Escherichia coli in vivo and in vitro. J Bacteriol. 1973 Jan;113(1):42–50. doi: 10.1128/jb.113.1.42-50.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES