Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1979 Aug 1;150(2):246–255. doi: 10.1084/jem.150.2.246

Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells

PMCID: PMC2185627  PMID: 88499

Abstract

Human T-cell blasts were generated by stimulation with mitogens and antigens. A proportion of these blasts expressed Ia antigens detectable by immunofluorescence with both allo- and hetero-antiserums. The maximal expression of Ia antigens was delayed and usually occurred after the peak of blastogenesis. Among the three mitogens used, pokeweed mitogen (PWM) was most effective in giving a high percentage and intense Ia staining of T-cell blasts. Phytohemagglutinin and concanavalin A blasts gave weaker and lower percentages of Ia staining. Activation by alloantigens and soluble antigens such as tetanus toxoid and purified protein derivative resulted in Ia expression on T cells comparable to PWM stimulation. Depletion of Ia+ cells from freshly isolated T cells with anti-Ia and complement decreased subsequent Ia expression, suggesting that a proportion of Ia+ blasts were derived from Ia-bearing peripheral blood T cells. When the specificities of the Ia antigens on T-cell blasts were examined with alloantiserums, it was evident that the T blasts expressed similar HLA-DR determinants to those on B cells from the same donor; occasional minor differences between stimulated T cells and autologous B-cell lines or fresh B cells were encountered.

Full Text

The Full Text of this article is available as a PDF (666.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodmer W. F. HLA: a super supergene. Harvey Lect. 1978;72:91–138. [PubMed] [Google Scholar]
  2. Evans R. L., Faldetta T. J., Humphreys R. E., Pratt D. M., Yunis E. J., Schlossman S. F. Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens. J Exp Med. 1978 Nov 1;148(5):1440–1445. doi: 10.1084/jem.148.5.1440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fu S. M., Chiorazzi N., Wang C. Y., Montrazeri G., Kunkel H. G., Ko H. S., Gottlieb A. B. Ia-bearing T lymphocytes in man. Their identification and role in the generation of allogeneic helper activity. J Exp Med. 1978 Nov 1;148(5):1423–1428. doi: 10.1084/jem.148.5.1423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibofsky A., Winchester R. J., Patarroyo M., Fotino M., Kunkel H. G. Disease associations of the Ia-like human alloantigens. Contrasting patterns in rheumatoid arthritis and systemic lupus erythematosus. J Exp Med. 1978 Dec 1;148(6):1728–1732. doi: 10.1084/jem.148.6.1728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gillis S., Baker P. E., Ruscetti F. W., Smith K. A. Long-term culture of human antigen-specific cytotoxic T-cell lines. J Exp Med. 1978 Oct 1;148(4):1093–1098. doi: 10.1084/jem.148.4.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Huber Ch, Wigzell H. A simple rosette assay for demonstration of complement receptor sites using complement-coated zymosan beads. Eur J Immunol. 1976 Jun;5(6):432–435. doi: 10.1002/eji.1830050616. [DOI] [PubMed] [Google Scholar]
  7. Hurley J. N., Fu S. M., Kunkel H. G., McKenna G., Scharff M. D. Lymphoblastoid cell lines from patients with chronic lymphocytic leukemia: identification of tumor origin by idiotypic analysis. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5706–5710. doi: 10.1073/pnas.75.11.5706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mann D. L., Abelson L., Harris S., Amos D. B. Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera. J Exp Med. 1975 Jul 1;142(1):84–89. doi: 10.1084/jem.142.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Metzgar R. S., Bertoglio J., Anderson J. K., Bonnard G. D., Ruscetti F. W. Detection of HLA-DRw (Ia-like) antigens on human T lymphocytes grown in tissue culture. J Immunol. 1979 Mar;122(3):949–953. [PubMed] [Google Scholar]
  10. Murphy D. B., Herzenberg L. A., Okumura K., Herzenberg L. A., McDevitt H. O. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):699–712. doi: 10.1084/jem.144.3.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nagy Z., Elliott B. E., Nabholz M., Krammer P. H., Pernis B. Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction. J Exp Med. 1976 Mar 1;143(3):648–659. doi: 10.1084/jem.143.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Niederhuber J. E., Frelinger J. A., Dine M. S., Shoffner P., Dugan E., Shreffler D. C. Effects of anti-Ia sera on mitogenic responses. II. Differential expression of the Ia marker on phytohemagglutinin and concanavalin A-reactive T cells. J Exp Med. 1976 Feb 1;143(2):372–381. doi: 10.1084/jem.143.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Suciu-Foca N., Susinno E., McKiernan P., Rohowsky C., Weiner J., Rubinstein P. DRw determinants on human T cells primed against allogeneic lymphocytes. Transplant Proc. 1978 Dec;10(4):845–848. [PubMed] [Google Scholar]
  14. Tada T., Taniguchi M., David C. S. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. doi: 10.1084/jem.144.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Winchester R. J., Wernet P., Kunkel H. G., Dupont B., Jersild C., Fu S. M. Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes. J Exp Med. 1975 Apr 1;141(4):924–929. [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES