Abstract
A macrophage-dependent, antigen-specific murine T-cell proliferation assay was utilized to examine the role of soluble products of murine and human adherent cells in the activation of T lymphocytes. Highly purified human leukocytic pyrogen, and supernates from both murine and human mononuclear phagocytes-macrophages stimulated the immune T-cell proliferative response to the multideterminant antigens dinitrophenyl- ovalbumin and keyhole limpet hemocyanin. The implications of these studies and the relationship of leukocytic pyrogen to human lymphocyte- activating factor are discussed.
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Selected References
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- Blyden G., Handschumacher R. E. Purification and properties of human lymphocyte activating factor (LAF). J Immunol. 1977 May;118(5):1631–1638. [PubMed] [Google Scholar]
- Bodel P. Spontaneous pyrogen production by mouse histiocytic and myelomonocytic tumor cell lines in vitro. J Exp Med. 1978 May 1;147(5):1503–15l6. [PMC free article] [PubMed] [Google Scholar]
- Calderon J., Kiely J. M., Lefko J. L., Unanue E. R. The modulation of lymphocyte functions by molecules secreted by macrophages. I. Description and partial biochemical analysis. J Exp Med. 1975 Jul 1;142(1):151–164. doi: 10.1084/jem.142.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dinarello C. A., Goldin N. P., Wolff S. M. Demonstration and characterization of two distinct human leukocytic pyrogens. J Exp Med. 1974 Jun 1;139(6):1369–1381. doi: 10.1084/jem.139.6.1369. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dinarello C. A., Renfer L., Wolff S. M. Human leukocytic pyrogen: purification and development of a radioimmunoassay. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4624–4627. doi: 10.1073/pnas.74.10.4624. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dinarello C. A., Renfer L., Wolff S. M. The production of antibody against human leukocytic pyrogen. J Clin Invest. 1977 Aug;60(2):465–472. doi: 10.1172/JCI108797. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dinarello C. A., Wolff S. M. Partial purification of human leukocytic pyrogen. Inflammation. 1977 Sep;2(3):179–189. doi: 10.1007/BF00917594. [DOI] [PubMed] [Google Scholar]
- Economou J. S., Shin H. S. Lymphocyte-activating factor. I. Generation and physicochemical characterization. J Immunol. 1978 Oct;121(4):1446–1452. [PubMed] [Google Scholar]
- Farr A. G., Dorf M. E., Unanue E. R. Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3542–3546. doi: 10.1073/pnas.74.8.3542. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gery I., Handschumacher R. E. Potentiation of the T lymphocyte response to mitogens. III. Properties of the mediator(s) from adherent cells. Cell Immunol. 1974 Mar 30;11(1-3):162–169. doi: 10.1016/0008-8749(74)90016-1. [DOI] [PubMed] [Google Scholar]
- Gery I., Waksman B. H. Potentiation of the T-lymphocyte response to mitogens. II. The cellular source of potentiating mediator(s). J Exp Med. 1972 Jul 1;136(1):143–155. doi: 10.1084/jem.136.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klempner M. S., Dinarello C. A., Gallin J. I. Human leukocytic pyrogen induces release of specific granule contents from human neutrophils. J Clin Invest. 1978 May;61(5):1330–1336. doi: 10.1172/JCI109050. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachman L. B., Hacker M. P., Handschumacher R. E. Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques. J Immunol. 1977 Dec;119(6):2019–2023. [PubMed] [Google Scholar]
- Lipsky P. E., Ziff M. Inhibition of antigen- and mitogen-induced human lymphocyte proliferation by gold compounds. J Clin Invest. 1977 Mar;59(3):455–466. doi: 10.1172/JCI108660. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mizel S. B., Oppenheim J. J., Rosenstreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes. J Immunol. 1978 May;120(5):1497–1503. [PubMed] [Google Scholar]
- Mizel S. B., Oppenheim J. J., Rosentreich D. L. Characterization of lymphocyte-activating factor (LAF) produced by a macrophage cell line, P388D1. II. Biochemical characterization of LAF induced by activated T cells and LPS. J Immunol. 1978 May;120(5):1504–1508. [PubMed] [Google Scholar]
- Oppenheim J. J., Shneyour A., Kook A. I. Enhancement of DNA synthesis and cAMP content of mouse thymocytes by mediator(s) derived from adherent cells. J Immunol. 1976 May;116(5):1466–1472. [PubMed] [Google Scholar]
- Rosenwasser L. J., Rosenthal A. S. Adherent cell function in murine T lymphocyte antigen recognition. I. A. macrophage-dependent T cell proliferation assay in the mouse. J Immunol. 1978 Jun;120(6):1991–1995. [PubMed] [Google Scholar]
- Rosenwasser L. J., Rosenthal A. S. Adherent cell function in murine T lymphocyte antigen recognition. II. Definition of genetically restricted and nonrestricted macrophage functions in T cell proliferation. J Immunol. 1978 Dec;121(6):2497–2501. [PubMed] [Google Scholar]
- Unanue E. R., Kíely J. M. Synthesis and secretion of a mitogenic protein by macrophages: description of a superinduction phenomenon. J Immunol. 1977 Sep;119(3):925–931. [PubMed] [Google Scholar]