Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1979 Sep 19;150(3):507–516. doi: 10.1084/jem.150.3.507

Suppression of IgE antibody production in SJL mice. IV. Interaction of primed and unprimed T cells

PMCID: PMC2185649  PMID: 314487

Abstract

The mechanism of selective anti-hapten IgE antibody production was studied in SJL mice. Using an adoptive transfer method of spleen cells into syngeneic recipients irradiated with a sublethal dose of 600 rads, it was demonstrated that for the suppression of anti-dinitrophenyl (DNP) IgE antibody production the interaction of two subsets of T cells is necessary. DNP-primed B cells and carrier-primed T helper cells are taken from donors primed with small amounts of DNP-carrier conjugates. Without injection of other cells, high titer and persistent anti-DNP antibodies are produced in the recipients. The two subsets of T cells that are active in suppression of IgE are taken from two types of donors: one donor is immunized (hyperprimed) with larger amounts of carrier protein twice, the other is an unprimed donor. The carrier for hyperpriming the first type of donor may be unrelated to the carrier used for priming the helper T cells. To bring about anti-DNP IgE suppression it is necessary that the animals should be challenged with the same DNP-carrier conjugate used for priming the B and T helper cells. If the hyperprimed donors were immunized with a heterologous, unrelated carrier, then this heterologous unconjugated carrier must also be injected together with the homologous DNP-carrier conjugate. In these conditions, anti-DNP IgE antibody production is suppressed, but the production of anti-DNP IgG1 antibody is not diminished.

Full Text

The Full Text of this article is available as a PDF (617.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Hugenberger J., McVay-Boudreau L., Eardley D. D., Kemp J., Shen F. W., Gershon R. K. Immunoregulatory circuits among T-cell sets. Identification of a subpopulation of T-helper cells that induces feedback inhibition. J Exp Med. 1978 Oct 1;148(4):871–877. doi: 10.1084/jem.148.4.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chiorazzi N., Fox D. A., Katz D. H. Hapten-specific IgE antibody responses in mice. VII. Conversion of IgE "non-responder" strains to IgE "responders" by elimination of suppressor T cell activity. J Immunol. 1977 Jan;118(1):48–54. [PubMed] [Google Scholar]
  3. Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feldmann M., Beverley P. C., Woody J., McKenzie I. F. T-T interactions in the induction of suppressor and helper T cells: analysis of membrane phenotype of precursor and amplifier cells. J Exp Med. 1977 Apr 1;145(4):793–801. doi: 10.1084/jem.145.4.793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Itaya T., Okumura K., Watanabe N., Ovary Z. Absence of correlation of Ig-1 allotype and IgE antibody suppression in SJL mice. J Immunol. 1978 May;120(5):1758–1759. [PubMed] [Google Scholar]
  6. Katz D. H. Regulation of IgE antibody production by serum molecules. III. Induction of suppressive activity by allogeneic lymphoid cell interactions and suppression of IgE synthesis by the allogeneic effect. J Exp Med. 1979 Feb 1;149(2):539–544. doi: 10.1084/jem.149.2.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kishimoto T., Hirai Y., Suemura M., Nakanishi K., Yamamura Y. Regulation of antibody response in different immunoglobulin classes. IV. Properties and functions of IgE class-specific" suppressor factor(s) released from DNP-mycobacterium-primed T cells. J Immunol. 1978 Nov;121(5):2106–2112. [PubMed] [Google Scholar]
  8. Kishimoto T., Hirai Y., Suemura M., Yamamura Y. Regulation of antibody response in different immunoglobulin classes. I. Selective suppression of anti-DNP IgE antibody response by preadministration of DNP-coupled mycobacterium. J Immunol. 1976 Aug;117(2):396–404. [PubMed] [Google Scholar]
  9. Kojima S., Ovary Z. Effect of Nippostrongylus brasiliensis infection on anti-hapten IgE antibody response in the mouse. I. Induction of carrier specific helper cells. Cell Immunol. 1975 Feb;15(2):274–286. doi: 10.1016/0008-8749(75)90006-4. [DOI] [PubMed] [Google Scholar]
  10. McDougal J. S., Shen F. W., Elster P. Generation of T helper cells in vitro. V. Antigen-specific Ly1+ T cells mediate the helper effect and induce feedback suppression. J Immunol. 1979 Feb;122(2):437–442. [PubMed] [Google Scholar]
  11. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  12. Muirhead D. Y., Cudkowicz G. Subpopulations of splenic T cells regulating an anti-hapten antibody response. II. Distinct functions of, and sequential requirement for, helper and amplifier cells. J Immunol. 1978 Jul;121(1):130–137. [PubMed] [Google Scholar]
  13. OVARY Z., BENACERRAF B. IMMUNOLOGICAL SPECIFICITY OF THE SECONDARY RESPONSE WITH DINITROPHENYLATED PROTEINS. Proc Soc Exp Biol Med. 1963 Oct;114:72–76. doi: 10.3181/00379727-114-28589. [DOI] [PubMed] [Google Scholar]
  14. Okumura K., Tada T. Regulation of homocytotropic antibody formation in the rat. VI. Inhibitory effect of thymocytes on the homocytotropic antibody response. J Immunol. 1971 Dec;107(6):1682–1689. [PubMed] [Google Scholar]
  15. Ovary Z., Caiazza S. S., Kojima S. PCA reactions with mouse antibodies in mice and rats. Int Arch Allergy Appl Immunol. 1975;48(1):16–21. doi: 10.1159/000231289. [DOI] [PubMed] [Google Scholar]
  16. Takatsu K., Ishizaka K. Reaginic antibody formation in the mouse. VII. Induction of suppressor T cells for IgE and IgG antibody responses. J Immunol. 1976 May;116(5):1257–1264. [PubMed] [Google Scholar]
  17. Tung A. S., Chiorazzi N., Katz D. H. Regulation of IgE antibody production by serum molecules. I. Serum from complete Freund's adjuvant-immune donors suppresses irradiation-enhanced IgE production in low responder mouse strains. J Immunol. 1978 Jun;120(6):2050–2059. [PubMed] [Google Scholar]
  18. Waltenbaugh C., Thèze J., Kapp J. A., Benacerraf B. Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT). III. Generation of suppressor T cells by a suppressive extract derived from GT-primed lymphoid cells. J Exp Med. 1977 Oct 1;146(4):970–985. doi: 10.1084/jem.146.4.970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Watanabe N., Kojima S., Ovary Z. Suppression of IgE antibody production in SJL mice. I. Nonspecific suppressor T cells. J Exp Med. 1976 Apr 1;143(4):833–845. doi: 10.1084/jem.143.4.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Watanabe N., Ovary Z. Enhancement of IgE antibody production in AKR mice. Int Arch Allergy Appl Immunol. 1978;57(6):554–559. doi: 10.1159/000232152. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES