Abstract
Delayed-type hypersensitivity (DTH) responses specific for the phosphorylcholine (PC) hapten were induced in BALB/c mice by immunization with syngeneic peritoneal exudate cells (PEC) coupled with diazotized phenyl-phosphoryl-choline. PC-specific DTH responses were elicited in such immunized mice after footpad challenge with PC- derivatized syngeneic spleen cells. Moreover, PC-immune lymph node cells could passively transfer PC-specific DTH responses to naive BALB/c mice and it was possible to demonstrate that the cells responsible for such passively transferred responses were T lymphocytes. Because the T-15 idiotypic determinant displayed on the TEPC-15 PC-binding myeloma protein is known to be a dominant idiotype associated with anti-PC antibody responses in BALB/c mice, an analysis was made of the effects of anti-T-15 idiotypic antibodies on the induction and expression of murine PC-specific DTH responses. Repeated injections of anti-T-15 idiotypic antiserum, raised in A/J mice by immunization with TEPC-15 myeloma protein, into recipient BALB/c mice both immediately before and after sensitization with PC-PEC virtually abolished the development of PC-specific DTH responses. Although administration of anti-T-15 antiserum effectively inhibited the induction phase of PC-specific DTH responses, these anti-idiotypic antibodies had no suppressive activity at the effector phase of these responses. The inhibition observed with anti-T-15 antibodies was highly specific for the PC hapten, and for PC-specific DTH responses of BALB/c but not A/J mice. Studies were conducted to address the possibility that anti-Id treatment induced suppressor T lymphocytes capable of specifically inhibiting the activity of PC-specific T cells participating in DTH responses. The results demonstrate that idiotype- specific suppressor T cells are, indeed, induced by treatment with anti- Id; moreover, such suppressor T cells, once induced, are highly effective in abrogating both the induction and the effector phases of PC-specific T cell-mediated DTH responses in BALB/c mice.
Full Text
The Full Text of this article is available as a PDF (920.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bach B. A., Greene M. I., Benacerraf B., Nisonoff A. Mechanisms of regulation of cell-mediated immunity. IV. Azobenzenearsonate-specific suppressor factor(s) bear cross-reactive idiotypic determinants the expression of which is linked to the heavy-chain allotype linkage group of genes. J Exp Med. 1979 May 1;149(5):1084–1098. doi: 10.1084/jem.149.5.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bach B. A., Sherman L., Benacerraf B., Greene M. I. Mechanisms of regulation of cell-mediated immunity. II. Induction and suppression of delayed-type hypersensitivity to azobenzenearsonate-coupled syngeneic cells. J Immunol. 1978 Oct;121(4):1460–1468. [PubMed] [Google Scholar]
- Binz H., Wigzell H. Antigen-binding, idiotypic receptors from T lymphocytes: an analysis of their biochemistry, genetics, and use as immunogens to produce specific immune tolerance. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):275–284. doi: 10.1101/sqb.1977.041.01.033. [DOI] [PubMed] [Google Scholar]
- Bona C., Paul W. E. Cellular basis of regulation of expression of idiotype. I. T-suppressor cells specific for MOPC 460 idiotype regulate the expression of cells secreting anti-TNP antibodies bearing 460 idiotype. J Exp Med. 1979 Mar 1;149(3):592–600. doi: 10.1084/jem.149.3.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bottomly K., Mathieson B. J., Mosier D. E. Anti-idiotype induced regulation of helper cell function for the response to phosphorylcholine in adult BALB/c mice. J Exp Med. 1978 Nov 1;148(5):1216–1227. doi: 10.1084/jem.148.5.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cazenave P. A. Idiotypic-anti-idiotypic regulation of antibody synthesis in rabbits. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5122–5125. doi: 10.1073/pnas.74.11.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chesebro B., Metzger H. Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972 Feb 29;11(5):766–771. doi: 10.1021/bi00755a014. [DOI] [PubMed] [Google Scholar]
- Cosenza H., Augustin A. A., Julius M. H. Idiotypes and anti-idiotypes as probes in analysis of immunoregulation. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):709–718. doi: 10.1101/sqb.1977.041.01.081. [DOI] [PubMed] [Google Scholar]
- Cosenza H., Köhler H. Specific inhibition of plaque formation to phosphorylcholine by antibody against antibody. Science. 1972 Jun 2;176(4038):1027–1029. doi: 10.1126/science.176.4038.1027. [DOI] [PubMed] [Google Scholar]
- Eichmann K. Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity. Eur J Immunol. 1975 Aug;5(8):511–517. doi: 10.1002/eji.1830050802. [DOI] [PubMed] [Google Scholar]
- Greene M. I., Bach B. A., Benacerraf B. Mechanisms of regulation of cell-mediated immunity. III. The characterization of azobenzenearsonate-specific suppressor T-cell-derived-suppressor factors. J Exp Med. 1979 May 1;149(5):1069–1083. doi: 10.1084/jem.149.5.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greene M. I., Sugimoto M., Benacerraf B. Mechanisms of regulation of cell-mediated immune responses. I. Effect of the route of immunization with TNP-coupled syngeneic cells on the induction and suppression of contact sensitivity to picryl chloride. J Immunol. 1978 May;120(5):1604–1611. [PubMed] [Google Scholar]
- Hart D. A., Wang A. L., Pawlak L. L., Nisonoff A. Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody. J Exp Med. 1972 Jun 1;135(6):1293–1300. doi: 10.1084/jem.135.6.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
- Ju S. T., Owen F. L., Nisonoff A. Structure and immunosuppression of a cross-reactive idiotype associated with anti-p-azophenylarsonate antibodies of strain-A mice. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):699–707. doi: 10.1101/sqb.1977.041.01.080. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Katz D. H., Osborne D. P., Jr The allogeneic effect in inbred mice. II. Establishment of the cellular interactions required for enhancement of antibody production by the graft-versus-host reaction. J Exp Med. 1972 Sep 1;136(3):455–465. doi: 10.1084/jem.136.3.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krawinkel U., Cramer M., Berek C., Hämmerling G., Black S. J., Rajewsky K., Eichmann K. On the structure of the T-cell receptor for antigen. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):285–294. doi: 10.1101/sqb.1977.041.01.034. [DOI] [PubMed] [Google Scholar]
- Lieberman R., Potter M., Mushinski E. B., Humphrey W., Jr, Rudikoff S. Genetics of a new IgVH (T15 idiotype) marker in the mouse regulating natural antibody to phosphorylcholine. J Exp Med. 1974 Apr 1;139(4):983–1001. doi: 10.1084/jem.139.4.983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKearn T. J., Stuart F. P., Fitch F. W. Anti-idiotypic antibody in rat transplantation immunity. I. Production of anti-idiotypic antibody in animals repeatedly immunized with alloantigens. J Immunol. 1974 Dec;113(6):1876–1882. [PubMed] [Google Scholar]
- Mäkelä O., Karjalainen K. Inherited immunoglobulin idiotypes of the mouse. Immunol Rev. 1977;34:119–138. doi: 10.1111/j.1600-065x.1977.tb00370.x. [DOI] [PubMed] [Google Scholar]
- Owen F. L., Nisonoff A. Effect of idiotype-specific suppressor T cells on primary and secondary responses. J Exp Med. 1978 Jul 1;148(1):182–194. doi: 10.1084/jem.148.1.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pierce S. K., Klinman N. R. Antibody-specific immunoregulation. J Exp Med. 1977 Aug 1;146(2):509–519. doi: 10.1084/jem.146.2.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potter M., Lieberman R. Common individual antigenic determinants in five of eight BALB-c IgA myeloma proteins that bind phosphoryl choline. J Exp Med. 1970 Oct 1;132(4):737–751. doi: 10.1084/jem.132.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strayer D. S., Cosenza H., Lee W. M., Rowley D. A., Köhler H. Neonatal tolerance induced by antibody against antigen-specific receptor. Science. 1974 Nov 15;186(4164):640–643. doi: 10.1126/science.186.4164.640. [DOI] [PubMed] [Google Scholar]