Abstract
The transformed clonal isolates of Indian muntjac diploid cells by a mouse sarcoma virus, 43-2XV, were tested for tumorigenicity in athymic nude mice. In spite of the indistinguishable transformed morphology, the tumorigenicity exhibited four different patterns: (a) no tumor formation; (b) slowly growing regressive tumor formation; (c) rapidly growing regressive tumor formation; and (d) rapidly growing progressive tumor formation. This demonstrates that the same diploid host cells transformed by the same virus reveal variable patterns of tumorigenic expression and some transformed host cells lack the tumorigenicity entirely. The findings that there are at least two chromosomes and four recombinant sites assigned for the proviral integrations of the sarcoma gene into the Indian muntjac gene (M. Hatanaka, R. Klein, R. Kominami, T. Oikawa, H. Okabe, N. Tsuchida, E. C. Connors, and A. Carrano. Transformation of Indian muntjac diploid cells by the proviral integration of sarcoma gene of a mouse retrovirus. Manuscript in preparation.) lead us to propose a hypothesis that variable expressions of tumorigenicity under the neutral background of immune responses, may arise from variable integrations of the sarcoma gene into the host chromosome.
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Selected References
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- Flanagan S. P. 'Nude', a new hairless gene with pleiotropic effects in the mouse. Genet Res. 1966 Dec;8(3):295–309. doi: 10.1017/s0016672300010168. [DOI] [PubMed] [Google Scholar]
- Freedman V. H., Shin S. I. Cellular tumorigenicity in nude mice: correlation with cell growth in semi-solid medium. Cell. 1974 Dec;3(4):355–359. doi: 10.1016/0092-8674(74)90050-6. [DOI] [PubMed] [Google Scholar]
- Haller O., Hansson M., Kiessling R., Wigzell H. Role of non-conventional natural killer cells in resistance against syngeneic tumour cells in vivo. Nature. 1977 Dec 15;270(5638):609–611. doi: 10.1038/270609a0. [DOI] [PubMed] [Google Scholar]
- Hatanaka M., Klein R., Lomg C. W., Gilden R. Mutants of nonproducer cell lines transformed by murine sarcoma virus. II. Relationship of tumorigenicity to presence of viral markers and rescuable sarcoma genome. J Exp Med. 1973 Aug 1;138(2):364–372. doi: 10.1084/jem.138.2.364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatanaka M., Klein R., Toni R., Walker J., Gilden R. Mutants of nonproducer cell lines transformed by murine sarcoma viruses. I. Induction, isolation, particle production, and tumorigenicity. J Exp Med. 1973 Aug 1;138(2):356–363. doi: 10.1084/jem.138.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones P. A., Laug W. E., Gardner A., Nye C. A., Fink L. M., Benedict W. F. In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells. Cancer Res. 1976 Aug;36(8):2863–2867. [PubMed] [Google Scholar]
- Pantelouris E. M. Absence of thymus in a mouse mutant. Nature. 1968 Jan 27;217(5126):370–371. doi: 10.1038/217370a0. [DOI] [PubMed] [Google Scholar]
- Povlsen C. O., Fialkow P. J., Klein E., Klein G., Rygaard J., Wiener F. Growth and antigenic properties of a biopsy-derived Burkitt's lymphoma in thymus-less (nude) mice. Int J Cancer. 1973 Jan 15;11(1):30–39. doi: 10.1002/ijc.2910110105. [DOI] [PubMed] [Google Scholar]
- Rygaard J., Povlsen C. O. Heterotransplantation of a human malignant tumour to "Nude" mice. Acta Pathol Microbiol Scand. 1969;77(4):758–760. doi: 10.1111/j.1699-0463.1969.tb04520.x. [DOI] [PubMed] [Google Scholar]
- Shin S. I., Freedman V. H., Risser R., Pollack R. Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4435–4439. doi: 10.1073/pnas.72.11.4435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stiles C. D., Desmond W., Jr, Sato G., Saier M. H., Jr Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4971–4975. doi: 10.1073/pnas.72.12.4971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsuchida N., Shih M. S., Gilden R. V., Hatanaka M. Sarcoma and helper-specific RNA tumor virus subunits in transformed nonproducer mouse cells activated to produce virus by treatment with bromodeoxyuridine. J Virol. 1974 Nov;14(5):1262–1267. doi: 10.1128/jvi.14.5.1262-1267.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsuchida N., Shih M., Gilden R. V., Hatanaka M. Mutants of nonproducer cell lines transformed by murine sarcoma virus. 3. Detection and characterization of RNA specific for helper and sarcoma viruses. J Exp Med. 1974 Jul 1;140(1):218–224. doi: 10.1084/jem.140.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wurster D. H., Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science. 1970 Jun 12;168(3937):1364–1366. doi: 10.1126/science.168.3937.1364. [DOI] [PubMed] [Google Scholar]